cDNA Clone ERK1

• Synonyms: MAPK3
• Plasmid: pGEX6P-1-ERK1
• Parent Plasmid: pGEX6P1
• Expressed: GST-ERK1
• Genbank: BC013992
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCGGCGGCGGCGGCTCAGGGGGGCGGGGGCGGGGAGCCCCGTAG
AACCGAGGGGGTCGGCCCGGGGGTCCCGGGGGAGGTGGAGATGGTGAAGG
GGCAGCCGTTCGACGTGGGCCCGCGCTACACGCAGTTGCAGTACATCGGC
GAGGGCGCGTACGGCATGGTCAGCTCGGCCTATGACCACGTGCGCAAGAC
TCGCGTGGCCATCAAGAAGATCAGCCCCTTCGAACATCAGACCTACTGCC
AGCGCACGCTCCGGGAGATCCAGATCCTGCTGCGCTTCCGCCATGAGAAT
GTCATCGGCATCCGAGACATTCTGCGGGCGTCCACCCTGGAAGCCATGAG
AGATGTCTACATTGTGCAGGACCTGATGGAGACTGACCTGTACAAGTTGC
TGAAAAGCCAGCAGCTGAGCAATGACCATATCTGCTACTTCCTCTACCAG
ATCCTGCGGGGCCTCAAGTACATCCACTCCGCCAACGTGCTCCACCGAGA
TCTAAAGCCCTCCAACCTGCTCATCAACACCACCTGCGACCTTAAGATTT
GTGATTTCGGCCTGGCCCGGATTGCCGATCCTGAGCATGACCACACCGGC
TTCCTGACGGAGTATGTGGCTACGCGCTGGTACCGGGCCCCAGAGATCAT
GCTGAACTCCAAGGGCTATACCAAGTCCATCGACATCTGGTCTGTGGGCT
GCATTCTGGCTGAGATGCTCTCTAACCGGCCCATCTTCCCTGGCAAGCAC
TACCTGGATCAGCTCAACCACATTCTGGGCATCCTGGGCTCCCCATCCCA
GGAGGACCTGAATTGTATCATCAACATGAAGGCCCGAAACTACCTACAGT
CTCTGCCCTCCAAGACCAAGGTGGCTTGGGCCAAGCTTTTCCCCAAGTCA
GACTCCAAAGCCCTTGACCTGCTGGACCGGATGTTAACCTTTAACCCCAA
TAAACGGATCACAGTGGAGGAAGCGCTGGCTCACCCCTACCTGGAGCAGT
ACTATGACCCGACGGATGAGCCAGTGGCCGAGGAGCCCTTCACCTTCGCC
ATGGAGCTGGATGACCTACCTAAGGAGCGGCTGAAGGAGCTCATCTTCCA
GGAGACAGCACGCTTCCAGCCCGGAGTGCTGGAGGCCCCCTAGGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAAAAQGGGGGEPRRTEGV
GPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAI
KKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYI
VQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS
NLLSNTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSK
GYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLN
CIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRIT
VEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETAR
FQPGVLEAP*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1-ERK1138.95 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU1509
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expression
• Low Temp: No