cDNA Clone ERK1

• Synonyms: MAPK3
• Plasmid: pMalp2X ERK1
• Parent Plasmid: pMALP2X
• Expressed: MBP-ERK1
• Genbank: BC013992
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCGCGGCGGCGGCGGCTCAGGGGGGCGGGGGCGGGGAGCCCCGTAG
AACCGAGGGGGTCGGCCCGGGGGTCCCGGGGGAGGTGGAGATGGTGAAGG
GGCAGCCGTTCGACGTGGGCCCGCGCTACACGCAGTTGCAGTACATCGGC
GAGGGCGCGTACGGCATGGTCAGCTCGGCCTATGACCACGTGCGCAAGAC
TCGCGTGGCCATCAAGAAGATCAGCCCCTTCGAACATCAGACCTACTGCC
AGCGCACGCTCCGGGAGATCCAGATCCTGCTGCGCTTCCGCCATGAGAAT
GTCATCGGCATCCGAGACATTCTGCGGGCGTCCACCCTGGAAGCCATGAG
AGATGTCTACATTGTGCAGGACCTGATGGAGACTGACCTGTACAAGTTGC
TGAAAAGCCAGCAGCTGAGCAATGACCATATCTGCTACTTCCTCTACCAG
ATCCTGCGGGGCCTCAAGTACATCCACTCCGCCAACGTGCTCCACCGAGA
TCTAAAGCCCTCCAACCTGCTCAGCAACACCACCTGCGACCTTAAGATTT
GTGATTTCGGCCTGGCCCGGATTGCCGATCCTGAGCATGACCACACCGGC
TTCCTGACGGAGTATGTGGCTACGCGCTGGTACCGGGCCCCAGAGATCAT
GCTGAACTCCAAGGGCTATACCAAGTCCATCGACATCTGGTCTGTGGGCT
GCATTCTGGCTGAGATGCTCTCTAACCGGCCCATCTTCCCTGGCAAGCAC
TACCTGGATCAGCTCAACCACATTCTGGGCATCCTGGGCTCCCCATCCCA
GGAGGACCTGAATTGTATCATCAACATGAAGGCCCGAAACTACCTACAGT
CTCTGCCCTCCAAGACCAAGGTGGCTTGGGCCAAGCTTTTCCCCAAGTCA
GACTCCAAAGCCCTTGACCTGCTGGACCGGATGTTAACCTTTAACCCCAA
TAAACGGATCACAGTGGAGGAAGCGCTGGCTCACCCCTACCTGGAGCAGT
ACTATGACCCGACGGATGAGCCAGTGGCCGAGGAGCCCTTCACCTTCGCC
ATGGAGCTGGATGACCTACCTAAGGAGCGGCTGAAGGAGCTCATCTTCCA
GGAGACAGCACGCTTCCAGCCCGGAGTGCTGGAGGCCCCCTAGGTCGAC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISEFGSAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKG
QPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQ
RTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLL
KSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLSNTTCDLKIC
DFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGC
ILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQS
LPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQY
YDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1508
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal MBP tag for bacterial expression