cDNA Clone DYRK1A

• Plasmid: pGEX-DYRK1A 1-499
• Parent Plasmid: pGEX4T1
• Expressed: GST-DYRK1A
• Genbank: X79769
• Tag: GST
• Species: Rat

Sequence of Insert

GGATCCCCGGGAATTCAGACGATGCATACAGGAGGAGAGACTTCAGCATG
CAAACCTTCATCTGTCCGGCTCGCACCGTCATTCTCATTCCATGCTGCTG
GCCTTCAGATGGCTGCACAGATGCCCCATTCACATCAGTACAGTGACCGT
CGCCAGCCAAACATAAGTGACCAGCAGGTTTCTGCCTTATCATATTCTGA
CCAGATTCAGCAACCTCTAACTAACCAGGTGATGCCTGACATTGTCATGT
TACAGAGGCGGATGCCCCAAACCTTCCGTGATCCAGCAACTGCTCCTCTG
AGAAAACTCTCTGTGGACTTGATCAAAACATACAAGCATATTAATGAGGT
TTACTATGCAAAAAAGAAGCGAAGACACCAACAGGGCCAGGGGGACGATT
CTAGTCATAAGAAGGAGCGGAAGGTTTACAACGATGGTTACGATGACGAT
AACTATGATTACATTGTAAAAAACGGAGAAAAGTGGATGGACCGTTATGA
AATCGACTCCTTAATAGGCAAAGGTTCATTTGGACAGGTTGTGAAAGCAT
ATGACAGAGTGGAACAAGAATGGGTCGCCATTAAAATCATCAAGAACAAG
AAGGCGTTTCTGAATCAAGCGCAGATAGAAGTGCGGCTGCTTGAGCTCAT
GAACAAACATGACACTGAGATGAAGTACTACATAGTGCATTTGAAACGCC
ACTTTATGTTTCGGAACCATCTCTGTCTAGTGTTTGAAATGCTGTCCTAT
AATCTCTATGATTTGTTGAGGAACACCAACTTCCGAGGGGTCTCTTTGAA
CCTGACACGAAAGTTTGCACAACAGATGTGCACAGCATTGCTTTTCCTTG
CGACTCCAGAACTTAGTATCATTCACTGTGACTTAAAGCCGGAGAACATC
CTTCTGTGTAACCCCAAACGAAGTGCAATCAAGATTGTTGACTTTGGCAG
CTCTTGTCAGTTGGGGCAGAGGATATACCAGTATATTCAGAGTCGCTTCT
ATCGGTCTCCAGAGGTGCTACTGGGAATGCCTTATGACCTTGCTATCGAC
ATGTGGTCCCTTGGATGTATCTTGGTTGAAATGCACACTGGAGAGCCTCT
GTTCAGTGGTGCCAATGAGGTGGATCAGATGAATAAAATAGTGGAAGTCT
TGGGCATCCCACCTGCTCATATTCTTGACCAAGCACCGAAAGCAAGAAAG
TTCTTTGAGAAGTTGCCTGATGGCACTTGGAGCTTAAAGAAGACCAAAGA
TGGAAAACGGGAGTACAAACCACCAGGAACCCGTAAACTTCATAATATTC
TTGGAGTGGAAACAGGAGGACCTGGCGGGCGGCGTGCTGGGGAGTCAGGT
CACACTGTAGCTGACTACTTGAAGTTCAAAGACCTCATTTTAAGGATGCT
TGATTATGACCCCAAAACTCGGATTCAACCTTATTATGCCCTGCAGCACA
GTTTTTTCAAGAAAACAGCTGATGAAGGTACAAACACAAGCAACAGTGTG
TCCACCAGCCCTGCCATGCATGGACTGTGAGACACATAGTCCCCAGGTGC
GCCAGCAGTTTCCGGCTCCTCTGGGCTGGTCAGGCACTGAAGCTCCTACA
CAAGTCACTGTTGAAACTCATCCTGTTCAAGAAACAACCTTTCATGTAGC
CCCTCAGCAGAATGCATTGCATCATCACCATGCTAGTGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSRRASVGSPGIQTMHTGGETSACKP
SSVRLAPSFSFHAAGLQMAAQMPHSHQYSDRRQPNISDQQVSALSYSDQI
QQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYY
AKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEID
SLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNK
HDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLT
RKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSC
QLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFS
GANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWSLKKTKDGK
REYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDY
DPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMHGL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU1501
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag encoding DYRK1a aa 1-500