cDNA Clone MEKK2

• Synonyms: MAP3K2
• Plasmid: pFBHTa D1-315 MEKK2
• Parent Plasmid: pSC-A
• Expressed: 6His-D1-315 MEKK2
• Genbank: AAD28547 and Q61083
• Tag: 6His
• Species: Human

Sequence of Insert

atctttaccccagagtatgatgatagtcgaataagaagaaggggaagtga
catagacaatcctactttgacCgtaatggacatcagcccacccagccgtt
cacctcgagctccgaccaactggagattgggcaaactgcttggccaagga
gcctttggaagggtctacctctgttatgatgttgatacaggaagagaatt
ggctgttaagcaagttcaatttgaccccgatagtcctgagaccagcaagg
aagtaaatgcacttgagtgtgaaattcagttgctgaaaaacttGctacat
gagcgaattgttcagtattatggctgtttgagggatccccaggaaaaaac
actttccatatttatggaatatatgccagggggttcaattaaggaccaat
taaaagcatatggcgctcttactgagaatgTGactaggaaatacacccgt
cagattctggagggtgtccattatttgcacagtaatatgattGtccaTAg
AgacatcaaaggcgcaaatatcctGcgagattcaacaggcaacgtTaaac
taggagattttggggccagcaaacggcttcagaccatctgtctctcaggg
acaggaatgaagtctgtcacgggcacaccatactggatgagccctgaagt
catcagtggaGaaggctatggaagaaaagcagacatctggagtgttgcat
gtaccgtggtagaaatgctaactgaaaagccgccttgggctgaatttgaa
gcaatggctgccatctttaaaatcgccactcagccaacaaacccaaagct
gccacctcatgtctcagactatactcgagatttcctcaaacggatttttg
tagaggccaaactgagaccttcagctgatgaactcttaaggcacatgttt
gtgcattatcactag

Amino Acid Sequence

MSYYHHHHHHDYDIPPTENLYFQGAMDPEFIFTPEYDDSRIRRRGSDIDN
PTLTVMDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVK
QVQFDPDSPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEKTLSI
FMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIK
GANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISG
EGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPH
VSDYTRDFLKRIFVEAKLRPSADELLRHMFVHYH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU977
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N terminal 6His tag. Differs to both of the noted Genbank sequences which are believed to contain frameshifts