cDNA Clone SENP6

• Plasmid: pFBHTb SUSP1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-SUSP1
• Genbank: NP_056386
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCAAGATGGCGGCGGGCAAGAGCGGCGGTAGCGCAGGGGAGATTAC
TTTTCTGGAAGCTTTGGCTAGATCAGAGTCTAAGAGAGATGGAGGTTTTA
AAAATAATTGGAGCTTTGATCATGAAGAAGAAAGTGAAGGAGATACAGAT
AAAGATGGGACAAATCTGCTCAGTGTGGATGAAGATGAGGATTCTGAAAC
CTCAAAAGGAAAAAAGTTAAATCGTCGATCTGAAATTGTTGCTAATAGCT
CTGGTGAATTCATCTTGAAGACATATGTAAGACGAAACAAGTCTGAAAGT
TTTAAAACTTTGAAAGGCAACCCAATTGGACTTAACATGTTGAGCAACAA
TAAGAAATTGAGTGAAAATATGCAAAATACGTCATTATGTTCTGGAACTG
TAGTTCATGGTAGACGTTTTCATCATGCTCATGCACAGATACCAGTAGTA
AAAACAGCAGCCCAAAGCAGTCTGGACCGAAAAGAAAGGAAAGAATACCC
ACCTCATGTCCAAAAAGTTGAAATTAATCCTGTAAGGTTAAGTCGGCTCC
AAGGTGTTGAACGTATAATGAAGAAAACAGAAGAGTCCGAATCACAAGTG
GAGCCTGAAATTAAGAGGAAAGTACAACAGAAACGGCACTGTAGTACCTA
TCAGCCTACTCCTCCTCTATCTCCTGCTTCAAAAAAATGTTTAACCCATT
TAGAGGATTTGCAAAGAAATTGCAGACAAGCTATTACTTTGAATGAGTCT
ACTGGACCATTATTAAGAACGTCAATTCATCAGAATTCTGGAGGACAGAA
GTCACAAAACACAGGATTAACAACCAAGAAGTTTTATGGCAACAATGTGG
AAAAGGTTCCAATTGATATTATTGTGAATTGTGATGACAGTAAACACACT
TATTTACAGACTAATGGAAAAGTCATTTTACCTGGGGCAAAAATACCCAA
AATCACAAACTTGAAAGAAAGGAAAACAAGTTTGTCAGACCTAAATGATC
CAATCATTTTGTCCAGTGATGATGATGATGACAACGACAGAACTAACAGA
AGAGAAAGCATATCTCCTCAGCCTGCTGATTCAGCATGTTCTTCCCCTGC
ACCATCCACTGGAAAAGTAGAAGCAGCACTAAATGAAAATACTTGCAGAG
CAGAGCGTGAACTACGAAGCATTCCAGAAGACTCAGAGTTAAATACAGTT
ACATTGCCAAGAAAAGCAAGAATGAAAGACCAGTTTGGCAATTCTATTAT
CAACACACCTCTGAAACGTCGTAAAGTGTTTTCTCAAGAACCTCCAGATG
CTTTAGCTTTAAGCTGCCAAAGTTCCTTTGACAGTGTCATTTTAAACTGT
CGAAGTATACGAGTAGGAACACTCTTCCGGCTGTTAATAGAGCCTGTAAT
TTTTTGTTTAGATTTTATCAAGATACAGCTAGACGAACCAGACCATGATC
CTGTAGAGATTATATTAAATACCTCTGATCTAACTAAATGTGAATGGTGT
AATGTCCGAAAATTACCTGTAGTGTTTCTTCAAGCAATTCCAGCAGTTTA
TCAAAAGCTGAGCATCCAACTGCAAATGAATAAGGAGGATAAAGTTTGGA
ATGATTGTAAAGGAGTAAATAAATTAACAAATTTAGAAGAACAATATATA
ATTTTAATTTTTCAAAATGGCCTTGATCCTCCGGCAAATATGGTATTTGA
AAGTATCATTAATGAAATTGGTATAAAGAATAACATCTCCAATTTTTTTG
CGAAAATTCCCTTTGAAGAAGCTAATGGCAGACTTGTTGCCTGTACAAGA
ACCTATGAAGAGAGCATCAAAGGAAGTTGTGGGCAAAAGGAAAACAAAAT
TAAAACTGTATCATTTGAATCTAAAATACAACTTAGAAGCAAACAAGAAT
TTCAGTTTTTTGATGAAGAAGAAGAAACTGGAGAAAACCACACCATCTTC
ATTGGCCCAGTAGAAAAGTTGATAGTATATCCACCACCTCCAGCTAAGGG
AGGCATCTCTGTTACCAATGAGGACCTGCACTGTCTAAATGAAGGAGAAT
TTTTAAATGATGTTATTATAGACTTTTATTTGAAATACTTGGTGCTTGAA
AAACTGAAGAAGGAAGACGCTGACCGAATTCATATATTCAGTTCTTTTTT
CTATAAACGCCTTAATCAGAGAGAGAGGAGAAATCATGAAACAACTAATC
TGTCAATACAGCAAAAACGGCATGGGAGAGTAAAAACATGGACCCGGCAC
GTAGATATTTTTGAGAAGGATTTTATTTTTGTACCCCTTAATGAAGCTGC
ACACTGGTTTTTGGCTGTTGTTTGTTTCCCCGGTTTGGAAAAACCAAAGT
ATGAACCTAATCCTCATTACCATGAAAATGCTGTCATACAGAAATGTTCA
ACTGTAGAGGACAGTTGTATTTCTTCTTCAGCCAGTGAAATGGAGAGTTG
TTCACAAAACTCTTCTGCCAAGCCTGTAATTAAGAAGATGCTAAACAAAA
AACATTGCATAGCTGTAATTGATTCCAATCCTGGGCAGGAAGAAAGTGAC
CCTCGTTATAAGAGAAACATATGCAGTGTAAAATACAGTGTGAAAAAAAT
AAATCATACTGCGAGTGAAAATGAAGAATTCAATAAAGGAGAATCTACAT
CCCAGAAAGTTGCTGATAGGACTAAAAGTGAGAATGGCCTACAGAATGAA
AGTTTAAGTTCCACACATCATACAGATGGCTTAAGCAAAATCAGACTAAA
CTATAGCGATGAATCACCTGAAGCTGGTAAAATGCTTGAAGATGAACTCG
TCGACTTCTCAGAAGATCAGGATAACCAGGATGATAGCAGTGACGATGGA
TTCCTCGCTGATGACAACTGCAGTTCAGAAATAGGACAGTGGCATTTAAA
GCCTACTATCTGTAAACAACCTTGTATCCTACTTATGGACTCACTCCGAG
GCCCTTCTCGGTCAAATGTTGTCAAAATTTTAAGAGAGTATTTAGAAGTG
GAATGGGAAGTTAAAAAAGGAAGCAAAAGAAGTTTTTCCAAAGATGTTAT
GAAGGGCTCTAATCCAAAAGTACCACAGCAAAACAACTTCAGTGACTGTG
GTGTATATGTATTGCAGTATGTAGAGAGCTTTTTTGAGAATCCAATTCTC
AGTTTTGAACTACCTATGAATTTGGCAAACTGGTTTCCTCCACCAAGAAT
GAGAACAAAAAGAGAAGAAATCCGAAACATAATTCTGAAGCTACAGGAAG
ATCAGAGCAAAGAGAAAAGAAAGCATAAGGACACTTACTCAACAGAAGCA
CCTTTAGGCGAAGGAACAGAACAATGTGTCAATAGTATCTCAGATTGACC
ATTTCTGTTACTTGTCATTTCTACTTTCAGAAACTAAATGACTTTCAAAT
TTGGGTATAGACAATAAAGAACTGAAGTGCTCACTACTCAGTGATTTGGA
AATTTTGATGCTTGTATAAATGTCAGATAATTAATTTCCAAAGGCGTATG
TATTAAGTAAAAGTCTGTAAATATGTTAATGAGGCCAATTTTTCCAGCAT
TTATAATTATTTTTTTCACTTGTTAGGAAGCTTTTGTTATGTATTTTCTG
TTAATAGTACCTAAAATTGCAACTTCTAAACCCAAATAAAAAGAAAATAT
TTATAGGAGGAAATGATTAATTTGATATTCTTTAGTGAACTTGTTTAATT
CCTCAGTGGGTGTGACATATTTCATGGGAATATTCAAATATCTATGGTAA
TATTTTGACCCTTTATATTTGTTCTAAAATAAGTCAAAATGTGAAAATAA
TATTAAATCTAAGATATTTTGAACTAAGCATCTTTATATGCTTGTGTAAC
AGGAACAAAGTAACAGCCTTTCAATTCATATACTGCCTTGTGTTCAGTGA
ACCCAAGAAATGTAATAAATATTTGTAATTTTACACAAATATTTAAGAGG
AAAGAGTATTAAGAGCAATTCAAAAAAAGTAACCTTATACTACTAAAAAA
AAAATTCTTGCATATATTATCATCAAATGCATTTTTGAAGACATCAAAGA
CTCAGGTTAAAACTATTTTGGTAAGTGCAGCTTGAATTTCAAATATCCCG
TGTTACCTTTCTCTATTACAGCTTAAAGTATGCTACAATCTGTGTCATAT
AGTTAATTGATAAGCATTTTTAATCTGTGTAAACACAGGAATTTAAATAG
GAATTTACTATTTTTTTATAAAAAAAAAAAAAAAAAAAAAAAAAGGGCGG
CCGCTCG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSKMAAGKSGGSAGEITFLEALAR
SESKRDGGFKNNWSFDHEEESEGDTDKDGTNLLSVDEDEDSETSKGKKLN
RRSEIVANSSGEFILKTYVRRNKSESFKTLKGNPIGLNMLSNNKKLSENM
QNTSLCSGTVVHGRRFHHAHAQIPVVKTAAQSSLDRKERKEYPPHVQKVE
INPVRLSRLQGVERIMKKTEESESQVEPEIKRKVQQKRHCSTYQPTPPLS
PASKKCLTHLEDLQRNCRQAITLNESTGPLLRTSIHQNSGGQKSQNTGLT
TKKFYGNNVEKVPIDIIVNCDDSKHTYLQTNGKVILPGAKIPKITNLKER
KTSLSDLNDPIILSSDDDDDNDRTNRRESISPQPADSACSSPAPSTGKVE
AALNENTCRAERELRSIPEDSELNTVTLPRKARMKDQFGNSIINTPLKRR
KVFSQEPPDALALSCQSSFDSVILNCRSIRVGTLFRLLIEPVIFCLDFIK
IQLDEPDHDPVEIILNTSDLTKCEWCNVRKLPVVFLQAIPAVYQKLSIQL
QMNKEDKVWNDCKGVNKLTNLEEQYIILIFQNGLDPPANMVFESIINEIG
IKNNISNFFAKIPFEEANGRLVACTRTYEESIKGSCGQKENKIKTVSFES
KIQLRSKQEFQFFDEEEETGENHTIFIGPVEKLIVYPPPPAKGGISVTNE
DLHCLNEGEFLNDVIIDFYLKYLVLEKLKKEDADRIHIFSSFFYKRLNQR
ERRNHETTNLSIQQKRHGRVKTWTRHVDIFEKDFIFVPLNEAAHWFLAVV
CFPGLEKPKYEPNPHYHENAVIQKCSTVEDSCISSSASEMESCSQNSSAK
PVIKKMLNKKHCIAVIDSNPGQEESDPRYKRNICSVKYSVKKINHTASEN
EEFNKGESTSQKVADRTKSENGLQNESLSSTHHTDGLSKIRLNYSDESPE
AGKMLEDELVDFSEDQDNQDDSSDDGFLADDNCSSEIGQWHLKPTICKQP
CILLMDSLRGPSRSNVVKILREYLEVEWEVKKGSKRSFSKDVMKGSNPKV
PQQNNFSDCGVYVLQYVESFFENPILSFELPMNLANWFPPPRMRTKREEI
RNIILKLQEDQSKEKRKHKDTYSTEAPLGEGTEQCVNSISD

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU975
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N terminal 6His tag. n.b. insert has 3’ UTR region.