cDNA Clone GSK3 beta

• Synonyms: GSK3B
• Plasmid: pGEX6P-1-GSK3b Y216F
• Parent Plasmid: pGEX6P1
• Expressed: GST-GSK3b Y216F
• Genbank: L33801
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCAGATCTGCCACCATGGAGTTCATGCCCATGGAGTCAGGGCGGCC
CAGAACCACCTCCTTTGCGGAGAGCTGCAAGCCGGTGCAGCAGCCTTCAG
CTTTTGGCAGCATGAAAGTTAGCAGAGACAAGGACGGCAGCAAGGTGACA
ACAGTGGTGGCAACTCCTGGGCAGGGTCCAGACAGGCCACAAGAAGTCAG
CTATACAGACACTAAAGTGATTGGAAATGGATCATTTGGTGTGGTATATC
AAGCCAAACTTTGTGATTCAGGAGAACTGGTCGCCATCAAGAAAGTATTG
CAGGACAAGAGATTTAAGAATCGAGAGCTCCAGATCATGAGAAAGCTAGA
TCACTGTAACATAGTCCGATTGCGTTATTTCTTCTACTCCAGTGGTGAGA
AGAAAGATGAGGTCTATCTTAATCTGGTGCTGGACTATGTTCCGGAAACA
GTATACAGAGTTGCCAGACACTATAGTCGAGCCAAACAGACGCTCCCTGT
GATTTATGTCAAGTTGTATATGTATCAGCTGTTCCGAAGTTTAGCCTATA
TCCATTCCTTTGGAATCTGCCATCGGGATATTAAACCGCAGAACCTCTTG
TTGGATCCTGATACTGCTGTATTAAAACTCTGTGACTTTGGAAGTGCAAA
GCAGCTGGTCCGAGGAGAACCCAATGTTTCGTTTATCTGTTCTCGGTACT
ATAGGGCACCAGAGTTGATCTTTGGAGCCACTGATTATACCTCTAGTATA
GATGTATGGTCTGCTGGCTGTGTGTTGGCTGAGCTGTTACTAGGACAACC
AATATTTCCAGGGGATAGTGGTGTGGATCAGTTGGTAGAAATAATCAAGG
TCCTGGGAACTCCAACAAGGGAGCAAATCAGAGAAATGAACCCAAACTAC
ACAGAATTTAAATTCCCTCAAATTAAGGCACATCCTTGGACTAAGGTCTT
CCGACCCCGAACTCCACCGGAGGCAATTGCACTGTGTAGCCGTCTGCTGG
AGTATACACCAACTGCCCGACTAACACCACTGGAAGCTTGTGCACATTCA
TTTTTTGATGAATTACGGGACCCAAATGTCAAACTACCAAATGGGCGAGA
CACACCTGCACTCTTCAACTTCACCACTCAAGAACTGTCAAGTAATCCAC
CTCTGGCTACCATCCTTATTCCTCCTCATGCTCGGATTCAAGCAGCTGCT
TCAACCCCCACAAATGCCACAGCAGCGTCAGATGCTAATACTGGAGACCG
TGGACAGACCAATAATGCTGCTTCTGCATCAGCTTCCAACTCCACCTGAA
CAGTCCCGAGCAGCCAGCTGCACAGGAAAAACCACCAGTTACTTGAGTGT
CACTCAGCAACACTGGTCACGTTTGGAAAGAATATTAAAAAAAAAAAAAA
AAGGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFRSATMEFMPMESGRPR
TTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSY
TDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDH
CNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVI
YVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQ
LVRGEPNVSFICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPI
FPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFR
PRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDT
PALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRG
QTNNAASASASNST*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU966
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST and EE tag