cDNA Clone PDK1

• Plasmid: pFastbac GST D1-51 PDK1
• Parent Plasmid: pFastbac GST
• Expressed: 6His-GST D1-51 PDK1
• Genbank: O15530
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGACGGCACTGCAGCCGAGCCTCGGCCCGGCGCCGGCTCCCTGCA
GCATGCCCAGCCTCCGCCGCAGCCTCGGAAGAAGCGGCCTGAGGACTTCA
AGTTTGGGAAAATCCTTGGGGAAGGCTCTTTTTCCACGGTTGTCCTGGCT
CGAGAACTGGCAACCTCCAGAGAATATGCGATTAAAATTCTGGAGAAGCG
ACATATCATAAAAGAGAACAAGGTCCCCTATGTAACCAGAGAGCGGGATG
TCATGTCGCGCCTGGATCACCCCTTCTTTGTTAAGCTTTACTTCACATTT
CAGGACGACGAGAAGCTGTATTTCGGCCTTAGTTATGCCAAAAATGGAGA
ACTACTTAAATATATTCGCAAAATCGGTTCATTCGATGAGACCTGTACCC
GATTTTACACGGCTGAGATCGTGTCTGCTTTAGAGTACTTGCACGGCAAG
GGCATCATTCACAGGGACCTTAAACCGGAAAACATTTTGTTAAATGAAGA
TATGCACATCCAGATCACAGATTTTGGAACAGCAAAAGTCTTATCCCCAG
AGAGCAAACAAGCCAGGGCCAACTCATTCGTGGGAACAGCGCAGTACGTT
TCTCCAGAGCTGCTCACGGAGAAGTCCGCCTGTAAGAGTTCAGACCTTTG
GGCTCTTGGATGCATAATATACCAGCTTGTGGCAGGACTCCCACCATTCC
GAGCTGGAAACGAGTATCTTATATTTCAGAAGATCATTAAGTTGGAATAT
GACTTTCCAGAAAAATTCTTCCCTAAGGCAAGAGACCTCGTGGAGAAACT
TTTGGTTTTAGATGCCACAAAGCGGTTAGGCTGTGAGGAAATGGAAGGAT
ACGGACCTCTTAAAGCACACCCGTTCTTCGAGTCCGTCACGTGGGAGAAC
CTGCACCAGCAGACGCCTCCGAAGCTCACCGCTTACCTGCCGGCTATGTC
GGAAGACGACGAGGACTGCTATGGCAATTATGACAATCTCCTGAGCCAGT
TTGGCTGCATGCAGGTGTCTTCGTCCTCCTCCTCACACTCCCTGTCAGCC
TCCGACACGGGCCTGCCCCAGAGGTCAGGCAGCAACATAGAGCAGTACAT
TCACGATCTGGACTCGAACTCCTTTGAACTGGACTTACAGTTTTCCGAAG
ATGAGAAGAGGTTGTTGTTGGAGAAGCAGGCTGGCGGAAACCCTTGGCAC
CAGTTTGTAGAAAATAATTTAATACTAAAGATGGGCCCAGTGGATAAGCG
GAAGGGTTTATTTGCAAGACGACGACAGCTGTTGCTCACAGAAGGACCAC
ATTTATATTATGTGGATCCTGTCAACAAAGTTCTGAAAGGTGAAATTCCT
TGGTCACAAGAACTTCGACCAGAGGCCAAGAATTTTAAAACTTTCTTTGT
CCACACGCCTAACAGGACGTATTATCTGATGGACCCCAGCGGGAACGCAC
ACAAGTGGTGCAGGAAGATCCAGGAGGTTTGGAGGCAGCGATACCAGAGC
CACCCGGACGCCGCTGTGCAGTGAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFDGTAAEPRPGAGSLQH
AQPPPQPRKKRPEDFKFGKILGEGSFSTVVLARELATSREYAIKILEKRH
IIKENKVPYVTRERDVMSRLDHPFFVKLYFTFQDDEKLYFGLSYAKNGEL
LKYIRKIGSFDETCTRFYTAEIVSALEYLHGKGIIHRDLKPENILLNEDM
HIQITDFGTAKVLSPESKQARANSFVGTAQYVSPELLTEKSACKSSDLWA
LGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYDFPEKFFPKARDLVEKLL
VLDATKRLGCEEMEGYGPLKAHPFFESVTWENLHQQTPPKLTAYLPAMSE
DDEDCYGNYDNLLSQFGCMQVSSSSSSHSLSASDTGLPQRSGSNIEQYIH
DLDSNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRK
GLFARRRQLLLTEGPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVH
TPNRTYYLMDPSGNAHKWCRKIQEVWRQRYQSHPDAAVQ*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU954
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST fusion for baculovirus expression