cDNA Clone BAD

• Plasmid: pGEX6P-1-BAD human
• Parent Plasmid: pGEX6P1
• Expressed: GST-BAD human
• Genbank: NM_004322
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTTCCAGATCCCAGAGTTTGAGCCGAGTGAGCAGGAAGACTCCAG
CTCTGCAGAGAGGGGCCTGGGCCCCAGCCCCGCAGGGGACGGGCCCTCAG
GCTCCGGCAAGCATCATCGCCAGGCCCCAGGCCTCCTGTGGGACGCCAGT
CACCAGCAGGAGCAGCCAACCAGCAGCAGCCATCATGGAGGCGCTGGGGC
TGTGGAGATCCGGAGTCGCCACAGCTCCTACCCCGCGGGGACGGAGGACG
ACGAAGGGATGGGGGAGGAGCCCAGCCCCTTTCGGGGCCGCTCGCGCTCG
GCGCCCCCCAACCTCTGGGCAGCACAGCGCTATGGCCGCGAGCTCCGGAG
GATGAGTGACGAGTTTGTGGACTCCTTTAAGAAGGGACTTCCTCGCCCGA
AGAGCGCGGGCACAGCAACGCAGATGCGGCAAAGCTCCAGCTGGACGCGA
GTCTTCCAGTCCTGGTGGGATCGGAACTTGGGCAGGGGAAGCTCCGCCCC
CTCCCAGTGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSFQIPEFEPSEQEDSSSAER
GLGPSPAGDGPSGSGKHHRQAPGLLWDASHQQEQPTSSSHHGGAGAVEIR
SRHSSYPAGTEDDEGMGEEPSPFRGRSRSAPPNLWAAQRYGRELRRMSDE
FVDSFKKGLPRPKSAGTATQMRQSSSWTRVFQSWWDRNLGRGSSAPSQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU953
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expression