cDNA Clone NDRG2

• Plasmid: pCMV-FLAG NDR2 AAA new
• Parent Plasmid: pCMV5
• Expressed: FLAG-NDR2 AAA new
• Genbank: AAL08624
• Tag: FLAG
• Species: Human

Sequence of Insert

GAATTCGCCACCATGGACTACAAGGACGACGATGACAAGGCGGAGCTGCA
GGAGGTGCAGATCACAGAGGAGAAGCCACTGTTGCCAGGACAGACGCCTG
AGGCGGCCAAGGAGGCTGAGTTAGCTGCCCGAATCCTCCTGGACCAGGGA
CAGACTCACTCTGTGGAGACACCATACGGCTCTGTCACTTTCACTGCCTA
TGGCACCCCCGCACCCGCAGCCCCTGCGATCCTTACCTACCACGATGTGG
GACTCAACTATAAATCTTGCTTCCAGCCACTGTTTCAGTTCGAGGACATG
CAGGAAATCATTCAGAACTTTGTGCGGGTTCATGTGGATGCCCCTGGAAT
GGAAGAGGGAGCCCCTGTGTTCCCTTTGGGATATCAGTACCCATCTCTGG
ACCAGCTTGCGGACATGATCCCTTGCGTCCTGCAGTACCTAAATTTCTCT
ACAATAATTGGAGTTGGTGTTGGAGCTGGAGCCTACATCCTGGCGAGATA
TGCTCTTAACCACCCGGACACTGTTGAAGGTCTTGTCCTCATCAACATTG
ATCCCAATGCCAAGGGTTGGATGGATTGGGCAGCCCACAAGCTAACAGGC
CTCACCTCTTCCATTCCGGAGATGATCCTTGGACATCTTTTCAGCCAGGA
AGAGCTCTCTGGAAATTCTGAGTTGATACAAAAGTACAGAAATATCATTA
CACATGCACCCAACCTGGATAACATTGAATTGTACTGGAACAGCTACAAC
AACCGCCGAGACCTGAACTTTGAGCGTGGAGGTAATATCACCCTCAGGTG
CCCTGTGATGCTGGTGGTAGGAGACCAAGCACCTCATGAAGATGCAGTGG
TGGAATGTAACTCAAAACTGGATCCCACCCAGACCTCGTTCCTCAAAATG
GCTGACTCCGGAGGTCAGCCCCAGCTGACTCAGCCAGGCAAGCTGACCGA
GGTCTTCAAGTACTTCCTGCAAGGCATGGGCTACATGGCCTCATCCTGCA
TGACTCGCCTGTCCCGGTCTCGTACAGCCTCTCTGACCAGTGCAGCATCC
GTTGATGGCAACCGGTCCCGCTCTCGCACCCTGTCCCAGAGCAGCGAGTC
TGGAACTCTTTCTTCGGGGCCCCCGGGGCACACCATGGAGGTCTCCTGTT
GAGAATTC

Amino Acid Sequence

MDYKDDDDKAELQEVQITEEKPLLPGQTPEAAKEAELAARILLDQGQTHS
VETPYGSVTFTAYGTPAPAAPAILTYHDVGLNYKSCFQPLFQFEDMQEII
QNFVRVHVDAPGMEEGAPVFPLGYQYPSLDQLADMIPCVLQYLNFSTIIG
VGVGAGAYILARYALNHPDTVEGLVLINIDPNAKGWMDWAAHKLTGLTSS
IPEMILGHLFSQEELSGNSELIQKYRNIITHAPNLDNIELYWNSYNNRRD
LNFERGGNITLRCPVMLVVGDQAPHEDAVVECNSKLDPTQTSFLKMADSG
GQPQLTQPGKLTEVFKYFLQGMGYMASSCMTRLSRSRTASLTSAASVDGN
RSRSRTLSQSSESGTLSSGPPGHTMEVSC*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU936
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal FLAG tag NDR2 . Mutations remove NLS.