cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pGEX6P-1-TPL2 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-TPL2 6His
• Genbank: P41279
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGAGTACATGAGCACTGGAAGTGACAATAAAGAAGAGATTGATTT
ATTAATTAAACATTTAAATGTGTCTGATGTAATAGACATTATGGAAAATC
TTTATGCAAGTGAAGAGCCAGCAGTTTATGAACCCAGTCTAATGACCATG
TGTCAAGACAGTAATCAAAACGATGAGCGTTCTAAGTCTCTGCTGCTTAG
TGGCCAAGAGGTACCATGGTTGTCATCAGTCAGATATGGAACTGTGGAGG
ATTTGCTTGCTTTTGCAAACCATATATCCAACACTGCAAAGCATTTTTAT
GGACAACGACCACAGGAATCTGGAATTTTATTAAACATGGTCATCACTCC
CCAAAATGGACGTTACCAAATAGATTCCGATGTTCTCCTGATCCCCTGGA
AGCTGACTTACAGGAATATTGGTTCTGATTTTATTCCTCGGGGCGCCTTT
GGAAAGGTATACTTGGCTCAAGATATAAAGACGAAGAAAAGAATGGCGTG
TAAACTGATCCCAGTAGATCAATTTAAGCCATCTGATGTGGAAATTCAGG
CTTGCTTCCGGCACGAGAACATCGCAGAGCTGTATGGCGCAGTCCTGTGG
GGTGAAACTGTCCATCTCTTTATGGAAGCAGGCGAGGGAGGGTCTGTTCT
GGAGAAACTGGAGAGCTGTGGACCAATGAGAGAATTTGAAATTATTTGGG
TGACAAAGCATGTTCTCAAGGGACTTGATTTTCTACACTCAAAGAAAGTG
ATCCATCATGATATTAAACCTAGCAACATTGTTTTCATGTCCACAAAAGC
TGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATGACCGAAGATGTCTATT
TTCCTAAGGACCTCCGAGGAACAGAGATTTACATGAGCCCAGAGGTCATC
CTGTGCAGGGGCCATTCAACCAAAGCAGACATCTACAGCCTGGGGGCCAC
GCTCATCCACATGCAGACGGGCACCCCACCCTGGGTGAAGCGCTACCCTC
GCTCAGCCTATCCCTCCTACCTGTACATAATCCACAAGCAAGCACCTCCA
CTGGAAGACATTGCAGATGACTGCAGTCCAGGGATGAGAGAGCTGATAGA
AGCTTCCCTGGAGAGAAACCCCAATCACCGCCCAAGAGCCGCAGACCTAC
TAAAACATGAGGCCCTGAACCCGCCCAGAGAGGATCAGCCACGCTGTACG
AGTCTGGACTCTGCCCTCTTGGAGCGCAAGAGGCTGCTGAGTAGGAAGGA
GCTGGAACTTCCTGAGAACATTGCTGATTCTTCGTGCACAGGAAGCACCG
AGGAATCTGAGATGCTCAAGAGGCAACGCTCTCTCTACATCGACCTCGGC
GCTCTGGCTGGCTACTTCAATCTTGTTCGGGGACCACCAACGCTTGAATA
TGGCCATCATCACCATCACCATTGAGAATTCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFEYMSTGSDNKEEIDLL
IKHLNVSDVIDIMENLYASEEPAVYEPSLMTMCQDSNQNDERSKSLLLSG
QEVPWLSSVRYGTVEDLLAFANHISNTAKHFYGQRPQESGILLNMVITPQ
NGRYQIDSDVLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRMACK
LIPVDQFKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGSVLE
KLESCGPMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMSTKAV
LVDFGLSVQMTEDVYFPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATL
IHMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPLEDIADDCSPGMRELIEA
SLERNPNHRPRAADLLKHEALNPPREDQPRCTSLDSALLERKRLLSRKEL
ELPENIADSSCTGSTEESEMLKRQRSLYIDLGALAGYFNLVRGPPTLEYG
HHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU924
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST and C terminal 6His tags