cDNA Clone JNK2

• Synonyms: MAPK9, SAPK1a
• Plasmid: pGEX6P-1-JNK2
• Parent Plasmid: pGEX6P1
• Expressed: GST-JNK2 6His
• Genbank: L31951
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCAGCGACAGTAAATGTGACAGTCAGTTTTATAGTGTGCAAGTGGC
AGACTCAACCTTCACTGTCCTAAAACGTTACCAGCAGCTGAAACCAATTG
GCTCTGGGGCCCAAGGGATTGTTTGTGCTGCATTTGATACAGTTCTTGGG
ATAAGTGTTGCAGTCAAGAAACTAAGCCGTCCTTTTCAGAACCAAACTCA
TGCAAAGAGAGCTTATCGTGAACTTGTCCTCTTAAAATGTGTCAATCATA
AAAATATAATTAGTTTGTTAAATGTGTTTACACCACAAAAAACTCTAGAA
GAATTTCAAGATGTGTATTTGGTTATGGAATTAATGGATGCTAACTTATG
TCAGGTTATTCACATGGAGCTGGATCATGAAAGAATGTCCTACCTTCTTT
ACCAGATGCTTTGTGGTATTAAACATCTGCATTCAGCTGGTATAATTCAT
AGAGATTTGAAGCCTAGCAACATTGTTGTGAAATCAGACTGCACCCTGAA
GATCCTTGACTTTGGCCTGGCCCGGACAGCGTGCACTAACTTCATGATGA
CCCCTTACGTGGTGACACGGTACTACCGGGCGCCCGAAGTCATCCTGGGT
ATGGGCTACAAAGAGAACGTTGATATCTGGTCAGTGGGTTGCATCATGGG
AGAGCTGGTGAAAGGTTGTGTGATATTCCAAGGCACTGACCATATTGATC
AGTGGAATAAAGTTATTGAGCAGCTGGGAACACCATCAGCAGAGTTCATG
AAGAAACTTCAGCCAACTGTGAGGAATTATGTCGAAAACAGACCAAAGTA
TCCTGGAATCAAATTTGAAGAACTCTTTCCAGATTGGATATTCCCATCAG
AATCTGAGCGAGACAAAATAAAAACAAGTCAAGCCAGAGATCTGTTATCA
AAAATGTTAGTGATTGATCCTGACAAGCGGATCTCTGTAGACGAAGCTCT
GCGTCACCCATACATCACTGTTTGGTATGACCCCGCCGAAGCAGAAGCCC
CACCACCTCAAATTTATGATGCCCAGTTGGAAGAAAGAGAACATGCAATT
GAAGAATGGAAAGAGCTAATTTACAAAGAAGTCATGGATTGGGAAGAAAG
AAGCAAGAATGGTGTTGTAAAAGATCAGCCTTCAGATGCAGCAGTAAGTA
GCAACGCCACTCCTTCTCAGTCTTCATCGATCAATGACATTTCATCCATG
TCCACTGAGCAGACGCTGGCCTCAGACACAGACAGCAGTCTTGATGCCTC
GACGGGACCCCTTGAAGGCTGTCGACATCATCACCATCACCATTGAGTCG
ACGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSDSKCDSQFYSVQVADSTF
TVLKRYQQLKPIGSGAQGIVCAAFDTVLGISVAVKKLSRPFQNQTHAKRA
YRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIH
MELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDF
GLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGELVK
GCVIFQGTDHIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIK
FEELFPDWIFPSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPY
ITVWYDPAEAEAPPPQIYDAQLEEREHAIEEWKELIYKEVMDWEERSKNG
VVKDQPSDAAVSSNATPSQSSSINDISSMSTEQTLASDTDSSLDASTGPL
EGCRHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU923
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST and C terminal 6His tags