cDNA Clone GSK3 beta

• Synonyms: GSK3B
• Plasmid: pFBHTb GSK3b S9A
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-GSK3b S9A
• Genbank: NP_002084
• Tag: 6His
• Species: Human

Sequence of Insert

GCCACCATGGAGTTCATGCCCATGGAGTCAGGGCGGCCCAGAACCACCGC
CTTTGCGGAGAGCTGCAAGCCGGTGCAGCAGCCTTCAGCTTTTGGCAGCA
TGAAAGTTAGCAGAGACAAGGACGGCAGCAAGGTGACAACAGTGGTGGCA
ACTCCTGGGCAGGGTCCAGACAGGCCACAAGAAGTCAGCTATACAGACAC
TAAAGTGATTGGAAATGGATCATTTGGTGTGGTATATCAAGCCAAACTTT
GTGATTCAGGAGAACTGGTCGCCATCAAGAAAGTATTGCAGGACAAGAGA
TTTAAGAATCGAGAGCTCCAGATCATGAGAAAGCTAGATCACTGTAACAT
AGTCCGATTGCGTTATTTCTTCTACTCCAGTGGTGAGAAGAAAGATGAGG
TCTATCTTAATCTGGTGCTGGACTATGTTCCGGAAACAGTATACAGAGTT
GCCAGACACTATAGTCGAGCCAAACAGACGCTCCCTGTGATTTATGTCAA
GTTGTATATGTATCAGCTGTTCCGAAGTTTAGCCTATATCCATTCCTTTG
GAATCTGCCATCGGGATATTAAACCGCAGAACCTCTTGTTGGATCCTGAT
ACTGCTGTATTAAAACTCTGTGACTTTGGAAGTGCAAAGCAGCTGGTCCG
AGGAGAACCCAATGTTTCGTATATCTGTTCTCGGTACTATAGGGCACCAG
AGTTGATCTTTGGAGCCACTGATTATACCTCTAGTATAGATGTATGGTCT
GCTGGCTGTGTGTTGGCTGAGCTGTTACTAGGACAACCAATATTTCCAGG
GGATAGTGGTGTGGATCAGTTGGTAGAAATAATCAAGGTCCTGGGAACTC
CAACAAGGGAGCAAATCAGAGAAATGAACCCAAACTACACAGAATTTAAA
TTCCCTCAAATTAAGGCACATCCTTGGACTAAGGTCTTCCGACCCCGAAC
TCCACCGGAGGCAATTGCACTGTGTAGCCGTCTGCTGGAGTATACACCAA
CTGCCCGACTAACACCACTGGAAGCTTGTGCACATTCATTTTTTGATGAA
TTACGGGACCCAAATGTCAAACTACCAAATGGGCGAGACACACCTGCACT
CTTCAACTTCACCACTCAAGAACTGTCAAGTAATCCACCTCTGGCTACCA
TCCTTATTCCTCCTCATGCTCGGATTCAAGCAGCTGCTTCAACCCCCACA
AATGCCACAGCAGCGTCAGATGCTAATACTGGAGACCGTGGACAGACCAA
TAATGCTGCTTCTGCATCAGCTTCCAACTCCACCTGA

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSATMEFMPMESGRPRTTAFAESC
KPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGN
GSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRY
FFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQ
LFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNV
SYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVD
QLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAI
ALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTT
QELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASA
SASNST*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU909
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N terminal 6His and EE tag