cDNA Clone GSK3 beta

• Synonyms: GSK3B
• Plasmid: pGEX6P-1-GSK3b S9A
• Parent Plasmid: pGEX6P1
• Expressed: GST-GSK3b S9A
• Genbank: NP_002084
• Tag: GST
• Species: Human

Sequence of Insert

GCCACCATGGAGTTCATGCCCATGGAGTCAGGGCGGCCCAGAACCACCGC
CTTTGCGGAGAGCTGCAAGCCGGTGCAGCAGCCTTCAGCTTTTGGCAGCA
TGAAAGTTAGCAGAGACAAGGACGGCAGCAAGGTGACAACAGTGGTGGCA
ACTCCTGGGCAGGGTCCAGACAGGCCACAAGAAGTCAGCTATACAGACAC
TAAAGTGATTGGAAATGGATCATTTGGTGTGGTATATCAAGCCAAACTTT
GTGATTCAGGAGAACTGGTCGCCATCAAGAAAGTATTGCAGGACAAGAGA
TTTAAGAATCGAGAGCTCCAGATCATGAGAAAGCTAGATCACTGTAACAT
AGTCCGATTGCGTTATTTCTTCTACTCCAGTGGTGAGAAGAAAGATGAGG
TCTATCTTAATCTGGTGCTGGACTATGTTCCGGAAACAGTATACAGAGTT
GCCAGACACTATAGTCGAGCCAAACAGACGCTCCCTGTGATTTATGTCAA
GTTGTATATGTATCAGCTGTTCCGAAGTTTAGCCTATATCCATTCCTTTG
GAATCTGCCATCGGGATATTAAACCGCAGAACCTCTTGTTGGATCCTGAT
ACTGCTGTATTAAAACTCTGTGACTTTGGAAGTGCAAAGCAGCTGGTCCG
AGGAGAACCCAATGTTTCGTATATCTGTTCTCGGTACTATAGGGCACCAG
AGTTGATCTTTGGAGCCACTGATTATACCTCTAGTATAGATGTATGGTCT
GCTGGCTGTGTGTTGGCTGAGCTGTTACTAGGACAACCAATATTTCCAGG
GGATAGTGGTGTGGATCAGTTGGTAGAAATAATCAAGGTCCTGGGAACTC
CAACAAGGGAGCAAATCAGAGAAATGAACCCAAACTACACAGAATTTAAA
TTCCCTCAAATTAAGGCACATCCTTGGACTAAGGTCTTCCGACCCCGAAC
TCCACCGGAGGCAATTGCACTGTGTAGCCGTCTGCTGGAGTATACACCAA
CTGCCCGACTAACACCACTGGAAGCTTGTGCACATTCATTTTTTGATGAA
TTACGGGACCCAAATGTCAAACTACCAAATGGGCGAGACACACCTGCACT
CTTCAACTTCACCACTCAAGAACTGTCAAGTAATCCACCTCTGGCTACCA
TCCTTATTCCTCCTCATGCTCGGATTCAAGCAGCTGCTTCAACCCCCACA
AATGCCACAGCAGCGTCAGATGCTAATACTGGAGACCGTGGACAGACCAA
TAATGCTGCTTCTGCATCAGCTTCCAACTCCACCTGA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMEFMPMESGRPRTTAFA
ESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKV
IGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVR
LRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLY
MYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGE
PNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDS
GVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPP
EAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFN
FTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNA
ASASASNST*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU908
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST and EE tag