cDNA Clone CHK1

• Synonyms: CHEK1
• Plasmid: pGEX6P-1-CHK1 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-CHK1 6His
• Genbank: BC004202
• Tag: GST
• Species: Human

Sequence of Insert

GCGAATTCATGGCAGTGCCCTTTGTGGAAGACTGGGACTTGGTGCAAACC
CTGGGAGAAGGTGCCTATGGAGAAGTTCAACTTGCTGTGAATAGAGTAAC
TGAAGAAGCAGTCGCAGTGAAGATTGTAGATATGAAGCGTGCCGTAGACT
GTCCAGAAAATATTAAGAAAGAGATCTGTATCAATAAAATGCTAAATCAT
GAAAATGTAGTAAAATTCTATGGTCACAGGAGAGAAGGCAATATCCAATA
TTTATTTCTGGAGTACTGTAGTGGAGGAGAGCTTTTTGACAGAATAGAGC
CAGACATAGGCATGCCTGAACCAGATGCTCAGAGATTCTTCCATCAACTC
ATGGCAGGGGTGGTTTATCTGCATGGTATTGGAATAACTCACAGGGATAT
TAAACCAGAAAATCTTCTGTTGGATGAAAGGGATAACCTCAAAATCTCAG
ACTTTGGCTTGGCAACAGTATTTCGGTATAATAATCGTGAGCGTTTGTTG
AACAAGATGTGTGGTACTTTACCATATGTTGCTCCAGAACTTCTGAAGAG
AAGAGAATTTCATGCAGAACCAGTTGATGTTTGGTCCTGTGGAATAGTAC
TTACTGCAATGCTCGCTGGAGAATTGCCATGGGACCAACCCAGTGACAGC
TGTCAGGAGTATTCTGACTGGAAAGAAAAAAAAACATACCTCAACCCTTG
GAAAAAAATCGATTCTGCTCCTCTAGCTCTGCTGCATAAAATCTTAGTTG
AGAATCCATCAGCAAGAATTACCATTCCAGACATCAAAAAAGATAGATGG
TACAACAAACCCCTCAAGAAAGGGGCAAAAAGGCCCCGAGTCACTTCAGG
TGGTGTGTCAGAGTCTCCCAGTGGATTTTCTAAGCACATTCAATCCAATT
TGGACTTCTCTCCAGTAAACAGTGCTTCTAGTGAAGAAAATGTGAAGTAC
TCCAGTTCTCAGCCAGAACCCCGCACAGGTCTTTCCTTATGGGATACCAG
CCCCTCATACATTGATAAATTGGTACAAGGGATCAGCTTTTCCCAGCCCA
CATGTCCTGATCATATGCTTTTGAATAGTCAGTTACTTGGCACCCCAGGA
TCCTCACAGAACCCCTGGCAGCGGTTGGTCAAAAGAATGACACGATTCTT
TACCAAATTGGATGCAGACAAATCTTATCAATGCCTGAAAGAGACTTGTG
AGAAGTTGGGCTATCAATGGAAGAAAAGTTGTATGAATCAGGTTACTATA
TCAACAACTGATAGGAGAAACAATAAACTCATTTTCAAAGTGAATTTGTT
AGAAATGGATGATAAAATATTGGTTGACTTCCGGCTTTCTAAGGGTGATG
GATTGGAGTTCAAGAGACACTTCCTGAAGATTAAAGGGAAGCTGATTGAT
ATTGTGAGCAGCCAGAAGGTTTGGCTTCCTGCCACACATCATCACCATCA
CCATTGAGCGGCCGCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMAVPFVEDWDLVQTLG
EGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHEN
VVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMA
GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNK
MCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQ
EYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYN
KPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSS
SQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSS
QNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTIST
TDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKIKGKLIDIV
SSQKVWLPATHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU904
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for Bacterial expression of N terminal GST tag C terminal 6His tag