cDNA Clone GSK3 beta

• Synonyms: GSK3B
• Plasmid: pFBHTbGSK3beta
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-GSK3beta
• Genbank: CAG38748
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGGATCTGCCACCATGGAGTTCA
TGCCCATGGAGTCAGGGCGGCCCAGAACCACCTCCTTTGCGGAGAGCTGC
AAGCCGGTGCAGCAGCCTTCAGCTTTTGGCAGCATGAAAGTTAGCAGAGA
CAAGGACGGCAGCAAGGTGACAACAGTGGTGGCAACTCCTGGGCAGGGTC
CAGACAGGCCACAAGAAGTCAGCTATACAGACACTAAAGTGATTGGAAAT
GGATCATTTGGTGTGGTATATCAAGCCAAACTTTGTGATTCAGGAGAACT
GGTCGCCATCAAGAAAGTATTGCAGGACAAGAGATTTAAGAATCGAGAGC
TCCAGATCATGAGAAAGCTAGATCACTGTAACATAGTCCGATTGCGTTAT
TTCTTCTACTCCAGTGGTGAGAAGAAAGATGAGGTCTATCTTAATCTGGT
GCTGGACTATGTTCCGGAAACAGTATACAGAGTTGCCAGACACTATAGTC
GAGCCAAACAGACGCTCCCTGTGATTTATGTCAAGTTGTATATGTATCAG
CTGTTCCGAAGTTTAGCCTATATCCATTCCTTTGGAATCTGCCATCGGGA
TATTAAACCGCAGAACCTCTTGTTGGATCCTGATACTGCTGTATTAAAAC
TCTGTGACTTTGGAAGTGCAAAGCAGCTGGTCCGAGGAGAACCCAATGTT
TCGTATATCTGTTCTCGGTACTATAGGGCACCAGAGTTGATCTTTGGAGC
CACTGATTATACCTCTAGTATAGATGTATGGTCTGCTGGCTGTGTGTTGG
CTGAGCTGTTACTAGGACAACCAATATTTCCAGGGGATAGTGGTGTGGAT
CAGTTGGTAGAAATAATCAAGGTCCTGGGAACTCCAACAAGGGAGCAAAT
CAGAGAAATGAACCCAAACTACACAGAATTTAAATTCCCTCAAATTAAGG
CACATCCTTGGACTAAGGTCTTCCGACCCCGAACTCCACCGGAGGCAATT
GCACTGTGTAGCCGTCTGCTGGAGTATACACCAACTGCCCGACTAACACC
ACTGGAAGCTTGTGCACATTCATTTTTTGATGAATTACGGGACCCAAATG
TCAAACTACCAAATGGGCGAGACACACCTGCACTCTTCAACTTCACCACT
CAAGAACTGTCAAGTAATCCACCTCTGGCTACCATCCTTATTCCTCCTCA
TGCTCGGATTCAAGCAGCTGCTTCAACCCCCACAAATGCCACAGCAGCGT
CAGATGCTAATACTGGAGACCGTGGACAGACCAATAATGCTGCTTCTGCA
TCAGCTTCCAACTCCACCTGA

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSATMEFMPMESGRPRTTSFAESC
KPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGN
GSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRY
FFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQ
LFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNV
SYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVD
QLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAI
ALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTT
QELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASA
SASNST*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU899
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag/ TEV protease site and EE tag