cDNA Clone CK1 alpha

• Synonyms: CSNK1A1
• Plasmid: pFastbac HTb CK1 alpha Y17N L45V
• Parent Plasmid: pFastBac HTb
• Expressed: 6His TEV CK1 alpha Y17N L45V
• Genbank: P48729 NM_001892.6 → NP_001883.4
• Tag: 6His
• Species: Human

Sequence of Insert

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSASSSGSKAEFIVGGKNKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKSKSNMKGF*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFastbac HTb CK1 alpha Y17N L45V363.05 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU894
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N terminal 6His tag; *Contains mutation at Y17N Compared to NCBI ref and several silent mutations*; use more recent clone as source
• Low Temp: No