cDNA Clone ERK5

• Synonyms: MAPK7
• Plasmid: pEBG-2T-ERK5 KD D200A
• Parent Plasmid: pEBG2T
• Expressed: GST-2T ERK5 KD D200A
• Genbank: Q13164
• Tag: GST
• Species: Human

Sequence of Insert

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSAEPLKEEDGEDGSAEPPGPVKAEP
AHTAASVAAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVSSARRR
LTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILRPTV
PYGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHS
AQVIHRDLKPSNLLVNENCELKIGAFGMARGLCTSPAEHQYFMTEYVATR
WYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIM
MVLGTPSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLG
RMLRFEPSARISAAAALRHPFLAKYHDPDDEPDCAPPFDFAFDREALTRE
RIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPGCPDVEMPSPWAP
SGDCAMESPPPAPPPCPGPAPDTIDLTLQPPPPVSEPAPPKKDGAISDNT
KAALKAALLKSLRSRLRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQ
ERAKEREKRRQERERKERGAGASGGPSTDPLAGLVLSDNDRSLLERWTRM
ARPAAPALTSVPAPAPAPTPTPTPVQPTSPPPGPVAQPTGPQPQSAGSTS
GPVPQPACPPPGPAPHPTGPPGPIPVPAPPQIATSTSLLAAQSLVPPPGL
PGSSTPGVLPYFPPGLPPPDAGGAPQSSMSESPDVNLVTQQLSKSQVEDP
LPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSASLSA
SLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDP*

Amino Acid Sequence

GGATCCATGGCCGAGCCTCTGAAGGAGGAAGACGGCGAGGACGGCTCTGC
GGAGCCCCCCGGGCCCGTGAAGGCCGAACCCGCCCACACCGCTGCCTCTG
TAGCGGCCAAGAACCTGGCCCTGCTTAAAGCCCGCTCCTTCGATGTGACC
TTTGACGTGGGCGACGAGTACGAGATCATCGAGACCATAGGCAACGGGGC
CTATGGAGTGGTGTCCTCCGCCCGCCGCCGCCTCACCGGCCAGCAGGTGG
CCATCAAGAAGATCCCTAATGCTTTCGATGTGGTGACCAATGCCAAGCGG
ACCCTCAGGGAGCTGAAGATCCTCAAGCACTTTAAACACGACAACATCAT
CGCCATCAAGGACATCCTGAGGCCCACCGTGCCCTATGGCGAATTCAAAT
CTGTCTACGTGGTCCTGGACCTGATGGAAAGCGACCTGCACCAGATCATC
CACTCCTCACAGCCCCTCACACTGGAACACGTGCGCTACTTCCTGTACCA
ACTGCTGCGGGGCCTGAAGTACATGCACTCGGCTCAGGTCATCCACCGTG
ACCTGAAGCCCTCCAACCTATTGGTGAATGAGAACTGTGAGCTCAAGATT
GGTGCCTTTGGTATGGCTCGTGGCCTGTGCACCTCGCCCGCTGAACATCA
GTACTTCATGACTGAGTATGTGGCCACGCGCTGGTACCGTGCGCCCGAGC
TCATGCTCTCTTTGCATGAGTATACACAGGCTATTGACCTCTGGTCTGTG
GGCTGCATCTTTGGTGAGATGCTGGCCCGGCGCCAGCTCTTCCCAGGCAA
AAACTATGTACACCAGCTACAGCTCATCATGATGGTGCTGGGTACCCCAT
CACCAGCCGTGATTCAGGCTGTGGGGGCTGAGAGGGTGCGGGCCTATATC
CAGAGCTTGCCACCACGCCAGCCTGTGCCCTGGGAGACAGTGTACCCAGG
TGCCGACCGCCAGGCCCTATCACTGCTGGGTCGCATGCTGCGTTTTGAGC
CCAGCGCTCGCATCTCAGCAGCTGCTGCCCTTCGCCACCCTTTCCTGGCC
AAGTACCATGATCCTGATGATGAGCCTGACTGTGCCCCGCCCTTTGACTT
TGCCTTTGACCGCGAAGCCCTCACTCGGGAGCGCATTAAGGAGGCCATTG
TGGCTGAAATTGAGGACTTCCATGCAAGGCGTGAGGGCATCCGCCAACAG
ATCCGCTTCCAGCCTTCTCTACAGCCTGTGGCTAGTGAGCCTGGCTGTCC
AGATGTTGAAATGCCCAGTCCCTGGGCTCCCAGTGGGGACTGTGCCATGG
AGTCTCCACCACCAGCCCCGCCACCATGCCCCGGCCCTGCACCTGACACC
ATTGATCTGACCCTGCAGCCACCTCCACCAGTCAGTGAGCCTGCCCCACC
AAAGAAAGATGGTGCCATCTCAGACAATACTAAGGCTGCCCTTAAAGCTG
CCCTGCTCAAGTCTTTGAGGAGCCGGCTCAGAGATGGCCCCAGCGCACCC
CTGGAGGCTCCTGAGCCTCGGAAGCCGGTGACAGCCCAGGAGCGCCAGCG
GGAGCGGGAGGAGAAGCGGCGGAGGCGGCAAGAACGAGCCAAGGAGCGGG
AGAAACGGCGGCAGGAGCGGGAGCGAAAGGAACGGGGGGCTGGGGCCTCT
GGGGGCCCCTCCACTGACCCCTTGGCTGGACTAGTGCTCAGTGACAATGA
CAGAAGCCTGTTGGAACGCTGGACTCGAATGGCCCGGCCCGCAGCCCCAG
CCCTCACCTCTGTGCCGGCCCCTGCCCCAGCGCCAACGCCAACCCCAACC
CCAGTCCAACCTACCAGTCCTCCTCCTGGCCCTGTAGCCCAGCCCACTGG
CCCGCAACCACAATCTGCGGGCTCTACCTCTGGCCCTGTACCCCAGCCTG
CCTGCCCACCCCCTGGCCCTGCACCCCACCCCACTGGCCCTCCTGGGCCC
ATCCCTGTCCCCGCGCCACCCCAGATTGCCACCTCCACCAGCCTCCTGGC
TGCCCAGTCACTTGTGCCACCCCCTGGGCTGCCTGGCTCCAGCACCCCAG
GAGTTTTGCCTTACTTCCCACCTGGCCTGCCGCCCCCAGACGCCGGGGGA
GCCCCTCAGTCTTCCATGTCAGAGTCACCTGACGTCAACCTTGTGACCCA
GCAGCTATCTAAGTCACAGGTGGAGGACCCCCTGCCCCCTGTGTTCTCAG
GCACACCAAAGGGCAGTGGGGCTGGCTACGGTGTTGGCTTTGACCTGGAG
GAATTCTTAAACCAGTCTTTCGACATGGGCGTGGCTGATGGGCCACAGGA
TGGCCAGGCAGATTCAGCCTCTCTCTCAGCCTCCCTGCTTGCTGACTGGC
TCGAAGGCCATGGCATGAACCCTGCCGATATTGAGTCCCTGCAGCGTGAG
ATCCAGATGGACTCCCCAATGCTGCTGGCTGACCTGCCTGACCTCCAGGA
CCCCTGAGCGGCCGC

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU845
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian construct to express GST N terminal fusion. Kinase dead mutation.