cDNA Clone EMAPL

• Plasmid: pGEX6P-1-EMAPL
• Parent Plasmid: pGEX6P1
• Expressed: GST-EMAPL
• Genbank: AK093146
• Tag: GST
• Species: Human

Sequence of Insert

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFEFATMYPYDVPDYAEL
VKAALAEALRLLRLQVPPSSLQGSGTPAPPGDSSLAAPPGLPPTCTPSLV
SRGTQTETEVELKSSPGPPGLSNGPPAPQGASEEPSGTQSEGGGSSSSGA
GSPGPPGILRPLQPPQRADTPRRNSSSSSSPSERPRQKLSRKAISSANLL
VRSGSTESRGGKDPLSSPGGPGSRRSNYNLEGISVKMFLRGRPITMYIPS
GIRSLEELPSGPPPETLSLDWVYGYRGRDSRSNLFVLRSGEVVYFIACVV
VLYRPGGGPGGPGGGGQRHYRGHTDCVRCLAVHPDGVRVASGQTAGVDKD
GKPLQPVVHIWDSETLLKLQEIGLGAFERGVGALAFSAADQGAFLCVVDN
SNEHMLSVWDCSRGMKLAEIKSTNDSVLAVGFNPRDSSCIVTSGKSHVHF
WNWSGGVGVPGNGTLTRKQGVFGKYKKPKFIPCFVFLPDGDILTGDSEGN
ILTWGRSPSDSKTPGRGGAKETYGIVAQAHAHEGSIFALCLRRDGTVLSG
GGRDRRLVQWGPGLVALQEAEIPEHFGAVRAIAEGLGSELLVGTTKNALL
RGDLAQGFSPVIQGHTDELWGLCTHPSQNRFLTCGHDRQLCLWDGESHAL
AWSIDLKETGLCADFHPSGAVVAVGLNTGRWLVLDTETREIVSDVIDGNE
QLSVVRYSPDGLYLAIGSHDNVIYIYSVSSDGAKSSRFGRCMGHSSFITH
LDWSKDGNFIMSNSGDYEILYWDVAGGCKQLKNRYESRDREWATYTCVLA
FTCTASGRTAPMGPTSTPCAAPTTSAWWRWPTTSAKGFHVYVPVRSCQGA
EPHVRGPRQPRDQRPIHARRLAPRLAGRQGRQHLPVASAGRWGRGAGARH
ALSNPLPVPRLLPRRLIAAWRDRLARRRGPAPPCPSLAQSPTTRGRLFPG
LTSRHSRSRIFLEGANGAPAHTV*

Amino Acid Sequence

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAACTGGT
AAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCC
CTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGCAGT
CTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAG
CCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGAC
CCCCTGGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAG
CCTAGCGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGTGGTGCTGG
CTCCCCTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCCACAGCGTG
CTGACACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCTCAGAGCGG
CCTCGGCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAACCTGTTAGT
GCGGTCCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCTCTCCAGCC
CTGGGGGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAGGCATCTCA
GTGAAGATGTTCCTTCGAGGGCGCCCCATTACCATGTACATCCCGTCTGG
CATCCGCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGAGACCCTCA
GCCTTGACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCTCTAATCTG
TTTGTGTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGTGTGGTGGT
GCTGTACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGAGGTGGCGGCCAGA
GACATTACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTGTTCACCCT
GATGGCGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGATAAGGATGG
AAAGCCCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGACGCTGTTGA
AACTGCAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTGGGGCCCTG
GCCTTTTCAGCTGCGGATCAGGGTGCCTTTCTTTGTGTGGTGGATAATTC
CAATGAGCACATGCTGTCGGTGTGGGACTGCAGCCGGGGAATGAAGCTGG
CTGAGATCAAGAGTACAAATGACTCAGTCCTGGCCGTTGGCTTCAACCCT
CGTGACAGCAGCTGCATCGTCACCAGTGGGAAATCTCACGTCCACTTCTG
GAATTGGAGTGGTGGAGTAGGGGTTCCTGGGAATGGGACCCTTACCCGGA
AACAGGGTGTCTTTGGGAAATACAAGAAACCCAAGTTTATCCCTTGCTTT
GTGTTCCTTCCGGATGGAGACATTCTCACTGGAGACTCAGAGGGGAACAT
TCTCACCTGGGGGCGGAGCCCTTCAGATTCCAAGACCCCAGGCAGGGGTG
GCGCCAAAGAGACCTATGGGATTGTGGCCCAGGCTCACGCTCATGAAGGT
TCTATCTTCGCCTTGTGTCTCCGGAGGGACGGGACAGTGCTGAGTGGTGG
CGGGCGGGACCGCCGGCTGGTACAGTGGGGGCCCGGGTTGGTGGCCCTCC
AGGAGGCTGAGATTCCCGAGCACTTCGGGGCCGTGCGAGCCATTGCTGAA
GGGCTTGGCTCTGAGCTGCTGGTGGGAACCACGAAGAATGCATTGCTGAG
GGGAGACCTGGCCCAGGGCTTCTCCCCTGTAATCCAGGGCCACACTGATG
AGCTCTGGGGGCTCTGCACACACCCCTCCCAGAACCGCTTCCTCACCTGC
GGCCACGACCGGCAGCTCTGCCTGTGGGATGGGGAGAGCCATGCACTGGC
CTGGAGCATCGACCTCAAGGAGACTGGTCTCTGTGCTGACTTCCACCCGA
GTGGGGCAGTTGTGGCCGTAGGACTGAACACGGGGAGGTGGTTGGTTTTG
GACACAGAGACCAGAGAGATCGTGTCTGATGTCATTGATGGCAATGAGCA
GCTCTCAGTGGTCCGGTACAGCCCAGATGGGTTGTACCTGGCCATTGGTT
CCCATGACAACGTGATCTACATCTATAGTGTTTCCAGTGATGGTGCCAAA
TCCAGCCGCTTTGGCCGCTGTATGGGTCACTCCAGCTTCATCACTCATCT
TGACTGGTCCAAGGATGGGAATTTCATCATGTCCAATTCTGGGGACTATG
AGATTCTTTACTGGGACGTGGCTGGAGGCTGCAAGCAGCTGAAGAATCGC
TATGAGAGCCGAGACCGGGAATGGGCTACCTACACCTGTGTGCTGGGCTT
TCACGTCTACGGCGTCTGGCCGGACGGCTCCGATGGGACCGACATCAACT
CCCTGTGCCGCTCCCACAACGAGCGCGTGGTGGCGGTG*CCGACGACTTC
TGCAAAGTGCATCTCTTCCAGTACCCGTGCGCTCGTGCCAAGGCGCCGAG
CCGCATGTACGGGGGCCACGGCAGCCACGTGACCAGCGTCCGATTCACGC
ACGACGACTCGCACCTCGTCTCGCTGGGCGGCAAGGACGCCAGCATCTTC
CAGTGGCGAGTGCTGGGCGCTGGGGGCGCGGGGCCGGCGCCCGCCACGCC
CTCTCGAACCCCCTCCCTGTCCCCCGCCTCCTCCCTCGACGTTTGATCGC
TGCCTGGCGGGACCGACTGGCCCGGCGGCGTGGCCCCGCCCCGCCCTGCC
CTTCCCTGGCCCAATCCCCCACGACTAGGGGCCGACTCTTTCCTGGACTG
ACTTCGAGACATTCCCGATCGCGCATTTTCCTGGAGGGCGCGAACGGCGC
CCCTGCACACACTGTTTAGGTCGAC

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU838
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/HA tag for bacterial expression. As genbank except insert of ; AFTCTASGRTAPMGPTSTPCAAPTTSAWWRWPTTSAK; after amino acid 752.