cDNA Clone PDE3A

• Plasmid: pGEX6P-1-PDE3a frag
• Parent Plasmid: pGEX6P1
• Expressed: GST-PDE3a frag
• Genbank: M91667
• Tag: GST
• Species: Human

Sequence of Insert

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMYPYDVPDYAGVLMIAL
TSAVRTVSLISLERFKVAWRPYLAYLAGVLGILLARYVEQILPQSAEAAP
REHLGSQLIAGTKEDIPVFKRRRRSSSVVSAEMSGCSSKSHRRTSLPCIP
REQLMGHSEWDHKRGPRGSQSSGTSITVDIAVMGEATASLPTSWQTLLFH
QTCATSLRAVSNLLSTQLTFQAIHKPRVNPVTSLSENYTCSDSEESSEKD
KLAIPKRLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSTSIK
LQEAPSSSPDSWNNPVMMTLT*

Amino Acid Sequence

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGGCGTCCT
CATGATCGCCTTGACTAGCGCGGTCAGGACCGTGTCCCTCATTTCCTTAG
AGAGGTTCAAGGTCGCCTGGAGACCTTACCTGGCGTACCTGGCCGGCGTG
CTGGGGATCCTCTTGGCCAGGTACGTGGAACAAATCTTGCCGCAGTCCGC
GGAGGCGGCTCCAAGGGAGCATTTGGGGTCCCAGCTGATTGCTGGGACCA
AGGAAGATATCCCGGTGTTTAAGAGGAGGAGGCGGTCCAGCTCCGTCGTG
TCCGCCGAGATGTCCGGCTGCAGCAGCAAGTCCCATCGGAGGACCTCCCT
GCCCTGTATACCGAGGGAACAGCTCATGGGGCATTCAGAATGGGACCACA
AACGAGGGCCAAGAGGATCACAGTCTTCAGGAACCAGTATTACTGTGGAC
ATCGCCGTCATGGGCGAAGCCACGGCCTCATTACCGACCTCCTGGCAGAC
CCTTCTCTTCCACCAAACGTGTGCCACATCCTTGAGAGCCGTGAGCAACT
TGCTCAGCACACAGCTCACCTTCCAGGCCATTCACAAGCCCAGAGTGAAT
CCCGTTACTTCGCTCAGTGAAAACTATACCTGTTCTGACTCTGAAGAGAG
CTCTGAAAAAGACAAGCTTGCTATTCCAAAGCGCCTGAGAAGGAGTTTGC
CTCCTGGCTTGTTGAGACGAGTTTCTTCCACTTGGACCACCACCACCTCG
GCCACAGGTCTACCCACCTTGGAGCCTGCACCAGTACGGAGAGACCGCAG
CACCAGCATCAAACTGCAGGAAGCACCTTCATCCAGTCCTGATTCTTGGA
ATAATCCAGTGATGATGACCCTCACCA

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU829
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/HA tag for bacterial expression