cDNA Clone HSP27

• Plasmid: pGEX6P-1-HSP27
• Parent Plasmid: pGEX6P1
• Expressed: GST-HSP27
• Genbank: NM_001540
• Tag: GST
• Species: Human

Sequence of Insert

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSTERRVPFSLLRGPSWDPFR
DWYPHSRLFDQAFGLPRLPEEWSQWLGGSSWPGYVRPLPPAAIESPAVAA
PAYSRALSRQLSSGVSEIRHTADRWRVSLDVNHFAPDELTVKTKDGVVEI
TGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPEGTLTVEAPMP
KLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK*

Amino Acid Sequence

GGATCCACCGAGCGCCGCGTCCCCTTCTCGCTCCTGCGGGGCCCCAGCTG
GGACCCCTTCCGCGACTGGTACCCGCATAGCCGCCTCTTCGACCAGGCCT
TCGGGCTGCCCCGGCTGCCGGAGGAGTGGTCGCAGTGGTTAGGCGGCAGC
AGCTGGCCAGGCTACGTGCGCCCCCTGCCCCCCGCCGCCATCGAGAGCCC
CGCAGTGGCCGCGCCCGCCTACAGCCGCGCGCTCAGCCGGCAACTCAGCA
GCGGGGTCTCGGAGATCCGGCACACTGCGGACCGCTGGCGCGTGTCCCTG
GATGTCAACCACTTCGCCCCGGACGAGCTGACGGTCAAGACCAAGGATGG
CGTGGTGGAGATCACCGGCAAGCACGAGGAGCGGCAGGACGAGCATGGCT
ACATCTCCCGGTGCTTCACGCGGAAATACACGCTGCCCCCCGGTGTGGAC
CCCACCCAAGTTTCCTCCTCCCTGTCCCCTGAGGGCACACTGACCGTGGA
GGCCCCCATGCCCAAGCTAGCCACGCAGTCCAACGAGATCACCATCCCAG
TCACCTTCGAGTCGCGGGCCCAGCTTGGGGGCCCAGAAGCTGCAAAATCC
GATGAGACTGCCGCCAAGTAAGAATTC

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU808
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express N terminal GST fusion.