cDNA Clone PI3K alpha

• Plasmid: pFB PI3K alpha
• Parent Plasmid: pFastbac HTc
• Expressed: 6His-PI3K alpha
• Genbank: U79143
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCACTCGTCACGGCCGCCAGTGTGCTGGAATTCGCCCTTGATCAGA
ACAATGCCTCCAAGACCATCATCAGGTGAACTGTGGGGCATCCACTTGAT
GCCCCCAAGAATCCTAGTAGAATGTTTACTACCAAATGGAATGATAGTGA
CTTTAGAATGCCTCCGTGAGGCTACATTAATAACCATAAAGCATGAACTA
TTTAAAGAAGCAAGAAAATACCCCCTCCATCAACTTCTTCAAGATGAATC
TTCTTACATTTTCGTAAGTGTTACCCAAGAAGCAGAAAGGGAAGAATTTT
TTGATGAAACAAGACGACTTTGTGACCTTCGGCTTTTTCAACCCTTTTTA
AAAGTAATTGAACCAGTAGGCAACCGTGAAGAAAAGATCCTCAATCGAGA
AATTGGTTTTGCTATCGGCATGCCAGTGTGTGAATTTGATATGGTTAAAG
ATCCAGAAGTACAGGACTTCCGAAGAAATATTCTGAACGTTTGTAAAGAA
GCTGTGGATCTTAGGGACCTCAATTCACCTCATAGTAGAGCAATGTATGT
CTATCCTCCAAATGTAGAATCTTCACCAGAATTGCCAAAGCACATATATA
ATAAATTAGATAAAGGGCAAATAATAGTGGTGATCTGGGTAATAGTTTCT
CCAAATAATGACAAGCAGAAGTATACTCTGAAAATCAACCATGACTGTGT
ACCAGAACAAGTAATTGCTGAAGCAATCAGGAAAAAAACTCGAAGTATGT
TGCTATCCTCTGAACAACTAAAACTCTGTGTTTTAGAATATCAGGGCAAG
TATATTTTAAAAGTGTGTGGATGTGATGAATACTTCCTAGAAAAATATCC
TCTGAGTCAGTATAAGTATATAAGAAGCTGTATAATGCTTGGGAGGATGC
CCAATTTGATGTTGATGGCTAAAGAAAGCCTCTATTCTCAACTGCCAATG
GACTGTTTTACAATGCCATCTTATTCCAGACGCATTTCCACAGCTACACC
ATATATGAATGGAGAAACATCTACAAAATCCCTTTGGGTTATAAATAGTG
CACTCAGAATAAAAATTCTTTGTGCAACCTACGTGAATGTAAATATTCGA
GACATTGATAAGATCTATGTTCGAACAGGTATCTACCATGGAGGAGAACC
CTTATGTGACAATGTGAACACTCAAAGAGTACCTTGTTCCAATCCCAGGT
GGAATGAATGGCTGAATTATGATATATACATTCCTGATCTTCCTCGTGCT
GCTCGACTTTGCCTTTCCATTTGCTCTGTTAAAGGCCGAAAGGGTGCTAA
AGAGGAACACTGTCCATTGGCATGGGGAAATATAAACTTGTTTGATTACA
CAGACACTCTAGTATCTGGAAAAATGGCTTTGAATCTTTGGCCAGTACCT
CATGGACTAGAAGATTTGCTGAACCCTATTGGTGTTACTGGATCAAATCC
AAATAAAGAAACTCCATGCTTAGAGTTGGAGTTTGACTGGTTCAGCAGTG
TGGTAAAGTTCCCAGATATGTCAGTGATTGAAGAGCATGCCAATTGGTCT
GTATCCCGAGAAGCAGGATTTAGCTATTCCCACGCAGGACTGAGTAACAG
ACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGAACAGCTCAAAG
CAATTTCTACACGAGATCCTCTCTCTGAAATCACTGAGCAGGAGAAAGAT
TTTCTATGGAGTCACAGACACTATTGTGTAACTATCCCCGAAATTCTACC
CAAATTGCTTCTGTCTGTTAAATGGAATTCTAGAGATGAAGTAGCCCAGA
TGTATTGCTTGGTAAAAGATTGGCCTCCAATCAAACCTGAACAGGCTATG
GAACTTCTGGACTGTAATTACCCAGATCCTATGGTTCGAGGTTTTGCTGT
TCGGTGCTTGGAAAAATATTTAACAGATGACAAACTTTCTCAGTATTTAA
TTCAGCTAGTACAGGTCCTAAAATATGAACAATATTTGGATAACTTGCTT
GTGAGATTTTTACTGAAGAAAGCATTGACTAATCAAAGGATTGGGCACTT
TTTCTTTTGGCATTTAAAATCTGAGATGCACAATAAAACAGTTAGCCAGA
GGTTTGGCCTGCTTTTGGAGTCCTATTGTCGTGCATGTGGGATGTATTTG
AAGCACCTGAATAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAAC
TGACATTCTCAAACAGGAGAAGAAGGATGAAACACAAAAGGTACAGATGA
AGTTTTTAGTTGAGCAAATGAGGCGACCAGATTTCATGGATGCTCTACAG
GGCTTTCTGTCTCCTCTAAACCCTGCTCATCAACTAGGAAACCTCAGGCT
TGAAGAGTGTCGAATTATGTCCTCTGCAAAAAGGCCACTGTGGTTGAATT
GGGAGAACCCAGACATCATGTCAGAGTTACTGTTTCAGAACAATGAGATC
ATCTTTAAAAATGGGGATGATTTACGGCAAGATATGCTAACACTTCAAAT
TATTCGTATTATGGAAAATATCTGGCAAAATCAAGGTCTTGATCTTCGAA
TGTTACCTTATGGTTGTCTGTCAATCGGTGACTGTGTGGGACTTATTGAG
GTGGTGCGAAATTCTCACACTATTATGCAAATTCAGTGCAAAGGCGGCTT
GAAAGGTGCACTGCAGTTCAACAGCCACACACTACATCAGTGGCTCAAAG
ACAAGAACAAAGGAGAAATATATGATGCAGCCATTGACCTGTTTACACGT
TCATGTGCTGGATACTGTGTAGCTACCTTCATTTTGGGAATTGGAGATCG
TCACAATAGTAACATCATGGTGAAAGACGATGGACAGCTGTTTCATATAG
ATTTTGGACACTTTTTGGATCACAAGAAGAAAAAATTTGGTTATAAACGA
GAACGTGTGCCATTTGTTTTGACACAGGATTTCTTAATAGTGATTAGTAA
AGGAGCCCAAGAATGCACAAAGACAAGAGAATTTGAGAGGTTTCAGGAGA
TGTGTTACAAGGCTTATCTAGCTATTCGACAGCATGCCAATCTCTTCATA
AATCTTTTCTCAATGATGCTTGGCTCTGGAATGCCAGAACTACAATCTTT
TGATGACATTGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGC
AAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATCATGGT
GGCTGGACAACAAAAATGGATTGGATCTTCCACACAATTAAACAGCATGC
ATTGAACTGAAAGATAACTGAGAAGGGCGAATTCTGCAGATATCCATCAC
ACTGGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIHSSRPPVCWNSPLIRTMPPRPS
SGELWGIHLMPPRILVECLLPNGMIVTLECLREATLITIKHELFKEARKY
PLHQLLQDESSYIFVSVTQEAEREEFFDETRRLCDLRLFQPFLKVIEPVG
NREEKILNREIGFAIGMPVCEFDMVKDPEVQDFRRNILNVCKEAVDLRDL
NSPHSRAMYVYPPNVESSPELPKHIYNKLDKGQIIVVIWVIVSPNNDKQK
YTLKINHDCVPEQVIAEAIRKKTRSMLLSSEQLKLCVLEYQGKYILKVCG
CDEYFLEKYPLSQYKYIRSCIMLGRMPNLMLMAKESLYSQLPMDCFTMPS
YSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYV
RTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSI
CSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLL
NPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGF
SYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDFLWSHRH
YCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAMELLDCNY
PDPMVRGFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNLLVRFLLKK
ALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLNRQV
EAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDFMDALQGFLSPLN
PAHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDD
LRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRNSHT
IMQIQCKGGLKGALQFNSHTLHQWLKDKNKGEIYDAAIDLFTRSCAGYCV
ATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVL
TQDFLIVISKGAQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMML
GSGMPELQSFDDIAYIRKTLALDKTEQEALEYFMKQMNDAHHGGWTTKMD
WIFHTIKQHALN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU800
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag