cDNA Clone S6K1

• Plasmid: pFB p70S6K 1-421 T412E
• Parent Plasmid: pFastbac1 mod
• Expressed: 6His-p70S6K 1-421 T412E
• Genbank: AAA42103.1
• Tag: 6His
• Species: Rat

Sequence of Insert

CATATGAGGCGACGAAGGAGGCGGGACGGCTTTTACCCAGCGCCTGACTT
CCGACACAGGGAAGCTGAGGACATGGCAGGAGTGTTTGACATAGACCTGG
ACCAGCCAGAGGATGCAGGCTCTGAGGATGAGCTGGAGGAGGGGGGTCAG
TTAAATGAAAGCATGGACCATGGGGGAGTTGGACCATATGAACTTGGCAT
GGAACATTGTGAGAAATTTGAAATCTCAGAAACTAGTGTGAACAGAGGGC
CAGAAAAAATCAGACCAGAATGTTTTGAGCTACTTCGGGTACTTGGTAAA
GGGGGCTATGGAAAGGTTTTTCAAGTACGAAAAGTAACAGGAGCAAATAC
TGGGAAGATATTTGCCATGAAGGTGCTTAAAAAGGCAATGATAGTAAGAA
ATGCTAAAGATACAGCTCATACAAAAGCAGAGCGGAATATTCTGGAGGAA
GTAAAGCATCCCTTCATTGTGGATTTAATTTATGCCTTTCAGACCGGTGG
AAAACTCTACCTCATCCTTGAGTATCTCAGTGGAGGAGAACTATTTATGC
AGTTAGAAAGAGAGGGGATATTCATGGAAGATACAGCTTGCTTTTACTTG
GCTGAAATCTCCATGGCTTTGGGGCATTTACATCAAAAGGGGATTATCTA
CAGAGACCTGAAGCCGGAGAACATCATGCTTAATCACCAAGGTCACGTGA
AGCTGACAGACTTTGGACTATGCAAAGAATCTATTCATGATGGAACAGTC
ACGCACACATTTTGTGGAACAATAGAATACATGGCCCCTGAAATCTTGAT
GAGAAGCGGCCACAACCGTGCTGTGGATTGGTGGAGTTTGGGAGCATTAA
TGTATGACATGCTGACTGGAGCACCTCCATTCACTGGGGAGAATAGAAAG
AAAACAATTGACAAAATCCTCAAATGTAAACTTAATTTGCCTCCCTACCT
CACACAAGAAGCTCGAGATCTGCTTAAAAAGCTGCTGAAAAGAAATGCTG
CTTCTCGTCTTGGAGCTGGCCCTGGGGATGCTGGAGAAGTCCAAGCGCAT
CCATTTTTTAGACACATTAACTGGGAAGAGCTTTTGGCTCGGAAGGTGGA
GCCCCCCTTTAAGCCTCTGTTGCAATCTGAAGAGGATGTGAGTCAGTTTG
ATTCAAAGTTTACTCGTCAGACACCTGTTGACAGCCCCGATGACTCAACT
CTCAGTGAAAGTGCCAACCAGGTCTTTCTGGGTTTCGAATACGTGGCTCC
ATCTGTACTTGAAAGTTGAGCGGCCGC

Amino Acid Sequence

MHHHHHHMRRRRRRDGFYPAPDFRHREAEDMAGVFDIDLDQPEDAGSEDE
LEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRPECFEL
LRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAE
RNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREGIFMED
TACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKES
IHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLTGAPPF
TGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDA
GEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTRQTPVD
SPDDSTLSESANQVFLGFEYVAPSVLES*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU784
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Insect cell expression with N terminal 6His tag, activating mutation and C terminal deletion.; Calc mol Weight 48583 Da