cDNA Clone PRAS40

• Plasmid: pGFP HA PRAS40
• Parent Plasmid: pEGFPC1
• Expressed: GFP HA PRAS40
• Genbank: BC007416
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGCGTCGGG
GCGCCCCGAGGAGCTGTGGGAGGCCGTGGTGGGGGCCGCTGAGCGCTTCC
GGGCCCGGACTGGCACGGAGCTGGTGCTGCTGACCGCGGCCCCGCCGCCA
CCACCCCGCCCGGGCCCCTGTGCCTATCCTGCCCATGGTCGAGGAGCCCT
GGCGGAGGCAGCGCGCCGTTGCCTCCACGACATCGCACTGGCCCACAGGG
CTGCCACTGCTGCTCGGCCTCCTGCGCCCCCACCAGCACCACAGCCACCC
AGTCCCACACCCAGCCCACCCCGGCCTACCCTGGCCAGAGAGGACAACGA
GGAGGACGAGGATGAGCCCACAGAGACAGAGACCTCCGGGGAGCAGCTGG
GCATTAGTGATAATGGAGGGCTCTTTGTGATGGATGAGGACGCCACCCTC
CAGGACCTTCCCCCCTTCTGTGAGTCAGACCCCGAGAGTACAGATGATGG
CAGCCTGAGCGAGGAGACCCCCGCCGGCCCCCCCACCTGCTCAGTGCCCC
CAGCCTCAGCCCTACCCACACAGCAGTACGCCAAGTCCCTGCCTGTGTCT
GTGCCCGTCTGGGGCTTCAAGGAGAAGAGGACAGAGGCGCGGTCATCAGA
TGAGGAGAATGGGCCGCCCTCTTCGCCCGACCTGGACCGCATCGCGGCGA
GCATGCGCGCGCTGGTGCTGCGAGAGGCCGAGGACACCCAGGTCTTCGGG
GACCTGCCACGGCCGCGGCTTAACACCAGCGACTTCCAGAAGCTGAAGCG
GAAATATTGAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSTGSATMYPYDVPDYAASGRPEELWEAVVGAAERFRARTG
TELVLLTAAPPPPPRPGPCAYPAHGRGALAEAARRCLHDIALAHRAATAA
RPPAPPPAPQPPSPTPSPPRPTLAREDNEEDEDEPTETETSGEQLGISDN
GGLFVMDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSVPPASAL
PTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRAL
VLREAEDTQVFGDLPRPRLNTSDFQKLKRKY*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU748
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/HA tag for mammalian expression. n.b. due to GC content sequence data is not as yet 100% verified.