cDNA Clone PRAS40

• Plasmid: pCMV5-HA PRAS40
• Parent Plasmid: pCMV5
• Expressed: HA-PRAS40
• Genbank: BC007416
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGCGTCGGG
GCGCCCCGAGGAGCTGTGGGAGGCCGTGGTGGGGGCCGCTGAGCGCTTCC
GGGCCCGGACTGGCACGGAGCTGGTGCTGCTGACCGCGGCCCCGCCGCCA
CCACCCCGCCCGGGCCCCTGTGCCTATCCTGCCCATGGTCGAGGAGCCCT
GGCGGAGGCAGCGCGCCGTTGCCTCCACGACATCGCACTGGCCCACAGGG
CTGCCACTGCTGCTCGGCCTCCTGCGCCCCCACCAGCACCACAGCCACCC
AGTCCCACACCCAGCCCACCCCGGCCTACCCTGGCCAGAGAGGACAACGA
GGAGGACGAGGATGAGCCCACAGAGACAGAGACCTCCGGGGAGCAGCTGG
GCATTAGTGATAATGGAGGGCTCTTTGTGATGGATGAGGACGCCACCCTC
CAGGACCTTCCCCCCTTCTGTGAGTCAGACCCCGAGAGTACAGATGATGG
CAGCCTGAGCGAGGAGACCCCCGCCGGCCCCCCCACCTGCTCAGTGCCCC
CAGCCTCAGCCCTACCCACACAGCAGTACGCCAAGTCCCTGCCTGTGTCT
GTGCCCGTCTGGGGCTTCAAGGAGAAGAGGACAGAGGCGCGGTCATCAGA
TGAGGAGAATGGGCCGCCCTCTTCGCCCGACCTGGACCGCATCGCGGCGA
GCATGCGCGCGCTGGTGCTGCGAGAGGCCGAGGACACCCAGGTCTTCGGG
GACCTGCCACGGCCGCGGCTTAACACCAGCGACTTCCAGAAGCTGAAGCG
GAAATATTGAGGATCC

Amino Acid Sequence

MYPYDVPDYAASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRP
GPCAYPAHGRGALAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTP
SPPRPTLAREDNEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLP
PFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVW
GFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPR
PRLNTSDFQKLKRKY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU747
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA tag for mammalian expression. n.b. due to GC content sequence data is not as yet 100% verified.