cDNA Clone ERK8

• Synonyms: MAPK15
• Plasmid: pEBG-2T-ERK8
• Parent Plasmid: pEBG2T
• Expressed: GST-ERK8
• Genbank: AY065978
• Tag: GST
• Species: Human

Sequence of Insert

GGATTCTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACTCAG
GCGGCAGCTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACC
GGAGGACTGGTGAGGTCGTGGCCATCAAGAAAATCTTTGATGCTTTTAGG
GATAAGACAGATGCCCAGAGAACATTCCGGGAAATCACGCTCCTCCAGGA
GTTTGGGGACCATCCCAACATCATCAGCCTCCTTGACGTGATCCGGGCAG
AGAACGACAGGGACATTTACCTGGTGTTTGAGTTTATGGACACTGACCTG
AACGCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTC
CATCTTCTACCAGCTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACG
TTGTGCACCGGGACCAGAAGCCGTCCAATGTGCTCCTGGATGCCAACTGC
ACAGTGAAGCTGTGTGACTTTGGCCTGGCCCGCTCCCTGGGCGACCTCCC
TGAGGGGCCTGAGGACCAGGCCGTGACAGAGTACGTGGCCACACGCTGGT
ACCGAGCACCGGAGGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTG
GACATGTGGAGTCTGGGCTGTATCCTGGGGGAGATGCTGCGGGGGAGACC
CCTGTTCCCCGGCACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGA
CCATCCCACCGCCATCTGAGGAGGACCTCCTGGCTCTCGGCTCAGGCTGC
CGTGCCTCTGTGCTGCACCAGCTGGGGTCCCGGCCACGACAGACGCTGGA
TGCCCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTCCTTAGGC
GACTCCTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTG
CAGCACCCCTACGTGCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACG
AGAGGCAGATGTGCGGCCCCGGGCACACGAAGGGGTCCAGCTCTCTGTGC
CTGAGTACCGCAGCCGCGTCTATCAGATGATCCTGGAGTGTGGAGGCAGC
AGCGGCACCTCGAGAGAGAAGGGCCCGGAGGGTGTCTCCCCAAGCCAGGC
ACACCTGCACAAACCCAGAGCCGACCCTCAGCTGCCTTCTAGGACACCTG
TGCAGGGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGACCCTGCC
GAGCACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGC
TCCCCTGCTCCAAACTGCTCTCCTAGGGAATGGGGAAGGGCCCCCTGGGG
CGAAGGAAGCGCCCCCCTTGACACTCTCGCTGGTGAAGCCAGGCGGGAGG
GGAGCTGCGCCCTCCCTGACCTCCCAGGCTGCGGCTCAGGTGGCCAACCA
GGCCCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGGGTGGCCA
GCGTACAACAGGTCCCTCCCCGGCTTCCTCCGGAGGCCCGGCCCGGCCGG
AGGATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGGGGGTGCCAGGGC
TTTGCTTGGAGGCTACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCAC
TGGGCCACCTGCCCCTGCTGGAGGGGCACCATGTGTGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSCTVVDPRIVRRYLLRRQLGQGAYG
IVWKAVDRRTGEVVAIKKIFDAFRDKTDAQRTFREITLLQEFGDHPNIIS
LLDVIRAENDRDIYLVFEFMDTDLNAVIRKGGLLQDVHVRSIFYQLLRAT
RFLHSGHVVHRDQKPSNVLLDANCTVKLCDFGLARSLGDLPEGPEDQAVT
EYVATRWYRAPEVLLSSHRYTLGVDMWSLGCILGEMLRGRPLFPGTSTLH
QLELILETIPPPSEEDLLALGSGCRASVLHQLGSRPRQTLDALLPPDTSP
EALDLLRRLLVFAPDKRLSATQALQHPYVQRFHCPSDEWAREADVRPRAH
EGVQLSVPEYRSRVYQMILECGGSSGTSREKGPEGVSPSQAHLHKPRADP
QLPSRTPVQGPRPRPQSSPGHDPAEHESPRAAKNVPRQNSAPLLQTALLG
NGEGPPGAKEAPPLTLSLVKPGGRGAAPSLTSQAAAQVANQALIRGDWNR
GGGVRVASVQQVPPRLPPEARPGRRMFSTSALQGAQGGARALLGGYSQAY
GTVCHSALGHLPLLEGHHV

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU741
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag.