cDNA Clone RSK1

• Synonyms: MAPKAP-K1a, RPS6KA1
• Plasmid: pMT RSK-1 574A
• Parent Plasmid: pFastBac HTb
• Expressed: HA-RSK-1 574A
• Genbank: EDL80690
• Tag: HA
• Species: Rat

Sequence of Insert

GAATTCGCGGCCGCCACCATGTACCCATACGATGTTCCAGATTACGCTCT
CGAGATGCCGCTCGCCCAGCTCAAGGAACCCTGGCCGCTCATGGAGCTGG
TGCCGCTGGACCCGGAGAATGGACAGGCTTCAGGGGAAGAAGCTGGACTT
CAGCCATCCAAGGATGAGGGCATCCTCAAGGAGATCTCTATCACACACCA
CGTCAAGGCTGGCTCTGAGAAGGCTGATCCATCCCATTTTGAGCTCCTCA
AGGTTCTGGGCCAAGGATCCTTTGGCAAAGTCTTCCTGGTACGCAAGGTC
ACCCGGCCTGACAATGGGCACTTGTATGCCATGAAAGTATTAAAGAAGGC
CACGCTGAAAGTGCGTGACCGTGTTCGGACCAAGATGGAGAGAGACATCC
TAGCTGACGTGAACCACCCCTTCGTAGTGAAACTGCACTATGCCTTCCAG
ACCGAGGGCAAGCTCTATCTTATTCTGGACTTTCTGCGTGGTGGAGACCT
GTTCACACGACTCTCAAAGGAGCAGGTTATGTTTACAGAGGAGGATGTGA
AGTTTTACCTGGCTGAGCTGGCACTGGGCCTGGACCACCTGCACAGCTTG
GGCATCATTTACAGAGACCTCAAGCCTGAGAATATCCTTTTGGATGAGGA
GGGCCACATCAAACTCACTGACTTTGGCCTGAGCAAGGAGGCCATTGACC
ACGAAAAGAAGGCCTATTCCTTCTGCGGGACGGTGGAGTACATGGCCCCC
GAGGTTGTCAACCGCCAGGGCCACACCCACAGTGCAGATTGGTGGTCCTA
TGGGGTGTTGATGTTTGAGATGCTGACGGGCTCCCTGCCCTTCCAGGGGA
AGGACCGGAAGGAGACCATGACCTTGATTTTGAAGGCAAAGCTAGGCATG
CCCCAGTTTCTGAGCACGGAAGCCCAGAGCCTCCTGCGGGCCCTGTTCAA
GAGGAATCCTGCCAACCGGCTTGGCTCAGGCCCCGATGGGGCTGAGGAAA
TTAAGAGACATATCTTCTACTCTACCATTGACTGGAATAAGCTCTACCGC
CGTGAGATCAAGCCACCTTTCAAGCCCGCTGTGGCCCAGCCGGATGACAC
CTTCTACTTTGATACCGAGTTCACGTCACGCACACCCAGGGATTCGCCGG
GCATCCCCCCCAGTGCTGGTGCCCATCAGCTCTTCCGTGGCTTCAGCTTC
GTGGCCACCGGTCTGATGGAGGATGACAGCAAGCCTCGGGCCACCCAGGC
TCCGCTGCACTCGGTGGTACAGCAACTCCACGGGAAGAACTTGGTTTTCA
GCGATGGCTACATAGTAAAGGAGACGATCGGCGTGGGCTCCTACTCTGTG
TGTAAGCGCTGTGTCCACAAGGCCACCAACATGGAGTACGCAGTCAAAGT
CATCGACAAAAGCAAAAGAGATCCCTCCGAAGAGATCGAGATTCTTCTGC
GGTATGGACAGCACCCCAACATCATCACCCTGAAAGATGTGTATGACGAC
AGTAAGCACGTATACCTGGTGACAGAGCTGATGAGGGGCGGGGAGCTGCT
GGATAAGATCCTACGGCAGAAATTCTTCTCAGAGCGGGAGGCCAGCTTCG
TCCTGTACACCATCAGCAAGACTGTGGAATACTTGCACTCCCAAGGGGTC
GTCCACAGGGACCTCAAACCCAGTAACATCCTGTATGTGGATGAGTCTGG
GAACCCCGGATGCCTACGAATATGCGACTTTGGCTTTGCCAAGCAGCTAC
GGGCTGAGAACGGGCTTCTCATGGCTCCTTGCTACACAGCCAACTTTGTG
GCACCTGAGGTGCTGAAGCGTCAGGGCTACGATGAAGGCTGTGACATATG
GAGCCTGGGCGTTCTGCTGTACACGATGCTGGCAGGATACACTCCATTTG
CCAATGGGCCCAGTGATACCCCAGAGGAGATCCTCACCCGGATCAGCAGT
GGGAAGTTCACCCTCAACGGGGGAAACTGGAACACGGTTTCAGAGACAGC
CAAGGACTTAGTATCTAAGATGCTGCATGTGGACCCCCACCAGCGCCTCA
CAGCCAAACAGGTTCTGCAGCACCCGTGGATCACCCAGAAAGACAAGCTC
CCCCAGAGCCAGTTGTCCCACCAAGACCTGCAGCTTGTGAAGGGGGCCAT
GGCAGCTACATATTCTGCACTCAGTAGCTCCAAACCCACCCCCCAGCTCA
AGCCAATCGAGTCGTCCATCCTGGCCCAGCGGCGGGTGAGGAAGCTGCCA
TCCACCACCCTGTGAACCGCAGCGCAGACAAACTCCTTGGAGGCAGAGTC
CTTCCCAGAGGGAGCAAGCCTGAGTCACAGGACCAAGTGGAATGGAGTCC
TAAAGGAAGCAACTAGCCCAGCTCACCCGTGCGGGTGTGAAGTGCCTTCC
TCCCCAGGACGGGCTCTTCTGGGCTCAGGCTCCATTGTGTGAAATCCACT
CACTGTACAAACTATTTTTTAAGAAAGGAAAAGAAAAATGACATCATTTA
CCATGGATTTTTTTTTTACAAGATCCATTTGCCTTTTTGGCCATTGCAGT
CCCAGGAGGAACACCCAGTCCCATGTGTGGCCAAGACTCCCGTGATAGCT
TTGGGACTCCGCCCCTCTGTTGGTCAAGGAGCCATCTGCACCCGCCTCCG
AGCAGTTCGGCGTTGCCTCTCAGAGTTGTCGACTGGCTCCTCAGCAGAAC
TTGGTGTCCCCAGCCATCTCTTTTTCCATTCTGTTCTGGGGTTCTCGAAC
CACTTTCTGCTAAGAGCCCGGGACCCCACCCTGTCAGCTCTTGGCTCCAG
GCACCAGCATCCACAGCGCCCATGCGCAGTGGGCCCCTGCAGTCAGAACG
GGCAGCCCCGTGGAGAGGAGACGGAGAGCACTTTTTGGGAGACTTCCTGT
CTGCCACTGGACAGAGTTCACAGGAGACCAGGGAGGTAGTCCACGGGGGA
TGAGGGCTTTTCCCTTTCCTCCTCAGCTGGTAACTCAGGTTCATCTGTCC
AAGGCCTTTCTAATAAACCTACAGTCCAGCCCGGGAATTCAAAGGCCTAC
GTCGACGAGCTCAACTAGT

Amino Acid Sequence

MYPYDVPDYALEMPLAQLKEPWPLMELVPLDPENGQASGEEAGLQPSKDE
GILKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDNG
HLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLY
LILDFLRGGDLFTRLSKEQVMFTEEDVKFYLAELALGLDHLHSLGIIYRD
LKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQ
GHTHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLST
EAQSLLRALFKRNPANRLGSGPDGAEEIKRHIFYSTIDWNKLYRREIKPP
FKPAVAQPDDTFYFDTEFTSRTPRDSPGIPPSAGAHQLFRGFSFVATGLM
EDDSKPRATQAPLHSVVQQLHGKNLVFSDGYIVKETIGVGSYSVCKRCVH
KATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDSKHVYL
VTELMRGGELLDKILRQKFFSEREASFVLYTISKTVEYLHSQGVVHRDLK
PSNILYVDESGNPGCLRICDFGFAKQLRAENGLLMAPCYTANFVAPEVLK
RQGYDEGCDIWSLGVLLYTMLAGYTPFANGPSDTPEEILTRISSGKFTLN
GGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWITQKDKLPQSQLS
HQDLQLVKGAMAATYSALSSSKPTPQLKPIESSILAQRRVRKLPSTTL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU679
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression. Differs to Genbank ref. Inserted Q at 223 and E551G