cDNA Clone ERK2

• Synonyms: MAPK1, MAPK2
• Plasmid: pGEX6P-3-ERK2 D168A
• Parent Plasmid: pGEX6P1
• Expressed: GST-ERK2 D168A
• Genbank: NM_002745
• Tag: GST

Sequence of Insert

gcgtcgacgcggcggcggcggcggcgggcgcgggcccggagatggtccgc
gggcaggtgttcgacgtggggccgcgctacaccaacctctcgtacatcgg
cgagggcgcctacggcatggtgtgctctgcttatgataatgtcaacaaag
ttcgagtagctatcaagaaaatcagcccctttgagcaccagacctactgc
cagagaaccctgagggagataaaaatcttactgcgcttcagacatgagaa
catcattggaatcaatgacattattcgagcaccaaccatcgagcaaatga
aagatgtatatatagtacaggacctcatggaaacagatctttacaagctc
ttgaagacacaacacctcagcaatgaccatatctgctattttctctacca
gatcctcagagggttaaaatatatccattcagctaacgttctgcaccgtg
acctcaagccttccaacctgctgctcaacaccacctgtgatctcaagatc
tgtgcctttggcctggcccgtgttgcagatccagaccatgatcacacagg
gttcctgacagaatatgtggccacacgttggtacagggctccagaaatta
tgttgaattccaagggctacaccaagtccattgatatttggtctgtaggc
tgcattctggcagaaatgctttctaacaggcccatctttccagggaagca
ttatcttgaccagctgaaacacattttgggtattcttggatccccatcac
aagaagacctgaattgtataataaatttaaaagctaggaactatttgctt
tctcttccacacaaaaataaggtgccatggaacaggctgttcccaaatgc
tgactccaaagctctggacttattggacaaaatgttgacattcaacccac
acaagaggattgaagtagaacaggctctggcccacccatatctggagcag
tattacgacccgagtgacgagcccatcgccgaagcaccattcaagttcga
catggaattggatgacttgcctaaggaaaagctcaaagaactaatttttg
aagagactgctagattccagccaggatacagatcttaagcggccgcgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQPLGSPNSRVDAAAAAAGAGPEMVR
GQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYC
QRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKL
LKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKI
CAFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVG
CILAEMLSNRPIFPGKHYLDQLKHILGILGSPSQEDLNCIINLKARNYLL
SLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQ
YYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU663
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag