cDNA Clone ERK8

• Synonyms: MAPK15
• Plasmid: pFBHTb ERK8
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-ERK8
• Genbank: AY065978
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACTCAG
GCGGCAGCTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACC
GGAGGACTGGTGAGGTCGTGGCCATCAAGAAAATCTTTGATGCTTTTAGG
GATAAGACAGATGCCCAGAGAACATTCCGGGAAATCACGCTCCTCCAGGA
GTTTGGGGACCATCCCAACATCATCAGCCTCCTTGACGTGATCCGGGCAG
AGAACGACAGGGACATTTACCTGGTGTTTGAGTTTATGGACACTGACCTG
AACGCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTC
CATCTTCTACCAGCTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACG
TTGTGCACCGGGACCAGAAGCCGTCCAATGTGCTCCTGGATGCCAACTGC
ACAGTGAAGCTGTGTGACTTTGGCCTGGCCCGCTCCCTGGGCGACCTCCC
TGAGGGGCCTGAGGACCAGGCCGTGACAGAGTACGTGGCCACACGCTGGT
ACCGAGCACCGGAGGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTG
GACATGTGGAGTCTGGGCTGTATCCTGGGGGAGATGCTGCGGGGGAGACC
CCTGTTCCCCGGCACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGA
CCATCCCACCGCCATCTGAGGAGGACCTCCTGGCTCTCGGCTCAGGCTGC
CGTGCCTCTGTGCTGCACCAGCTGGGGTCCCGGCCACGACAGACGCTGGA
TGCCCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTCCTTAGGC
GACTCCTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTG
CAGCACCCCTACGTGCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACG
AGAGGCAGATGTGCGGCCCCGGGCACACGAAGGGGTCCAGCTCTCTGTGC
CTGAGTACCGCAGCCGCGTCTATCAGATGATCCTGGAGTGTGGAGGCAGC
AGCGGCACCTCGAGAGAGAAGGGCCCGGAGGGTGTCTCCCCAAGCCAGGC
ACACCTGCACAAACCCAGAGCCGACCCTCAGCTGCCTTCTAGGACACCTG
TGCAGGGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGACCCTGCC
GAGCACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGC
TCCCCTGCTCCAAACTGCTCTCCTAGGGAATGGGGAAAGGCCCCCTGGGG
CGAAGGAAGCGCCCCCCTTGACACTCTCGCTGGTGAAGCCAAGCGGGAGG
GGAGCTGCGCCCTCCCTGACCTCCCAGGCTGCGGCTCAGGTGGCCAACCA
GGCCCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGGGTGGCCA
GCGTACAACAGGTCCCTCCCCGGCTTCCTCCGGAGGCCCGGCCCGGCCGG
AGGATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGGGGGTGCCAGGGC
TTTGCTTGGAGGCTACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCAC
TGGGCCACCTGCCCCTGCTGGAGGGGCACCATGTGTGAGAATTC

Amino Acid Sequence

HHHHHHDYDIPTTENLYFQGAMGSCTVVDPRIVRRYLLRRQLGQGAYGIV
WKAVDRRTGEVVAIKKIFDAFRDKTDAQRTFREITLLQEFGDHPNIISLL
DVIRAENDRDIYLVFEFMDTDLNAVIRKGGLLQDVHVRSIFYQLLRATRF
LHSGHVVHRDQKPSNVLLDANCTVKLCDFGLARSLGDLPEGPEDQAVTEY
VATRWYRAPEVLLSSHRYTLGVDMWSLGCILGEMLRGRPLFPGTSTLHQL
ELILETIPPPSEEDLLALGSGCRASVLHQLGSRPRQTLDALLPPDTSPEA
LDLLRRLLVFAPDKRLSATQALQHPYVQRFHCPSDEWAREADVRPRAHEG
VQLSVPEYRSRVYQMILECGGSSGTSREKGPEGVSPSQAHLHKPRADPQL
PSRTPVQGPRPRPQSSPGHDPAEHESPRAAKNVPRQNSAPLLQTALLGNG
ERPPGAKEAPPLTLSLVKPSGRGAAPSLTSQAAAQVANQALIRGDWNRGG
GVRVASVQQVPPRLPPEARPGRRMFSTSALQGAQGGARALLGGYSQAYGT
VCHSALGHLPLLEGHHV*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU662
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express 6His N terminal fusion