cDNA Clone ERK2

• Synonyms: MAPK1, MAPK2
• Plasmid: pGEX6P-3-ERK2
• Parent Plasmid: pGEX6P1
• Expressed: GST-ERK2
• Genbank: NM_002745
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCCCGGGTCGACGCGGCGGCGGCGGCGGCGGGCGCGGGCCCGGAGA
TGGTCCGCGGGCAGGTGTTCGACGTGGGGCCGCGCTACACCAACCTCTCG
TACATCGGCGAGGGCGCCTACGGCATGGTGTGCTCTGCTTATGATAATGT
CAACAAAGTTCGAGTAGCTATCAAGAAAATCAGCCCCTTTGAGCACCAGA
CCTACTGCCAGAGAACCCTGAGGGAGATAAAAATCTTACTGCGCTTCAGA
CATGAGAACATCATTGGAATCAATGACATTATTCGAGCACCAACCATCGA
GCAAATGAAAGATGTATATATAGTACAGGACCTCATGGAAACAGATCTTT
ACAAGCTCTTGAAGACACAACACCTCAGCAATGACCATATCTGCTATTTT
CTCTACCAGATCCTCAGAGGGTTAAAATATATCCATTCAGCTAACGTTCT
GCACCGTGACCTCAAGCCTTCCAACCTGCTGCTCAACACCACCTGTGATC
TCAAGATCTGTGACTTTGGCCTGGCCCGTGTTGCAGATCCAGACCATGAT
CACACAGGGTTCCTGACAGAATATGTGGCCACACGTTGGTACAGGGCTCC
AGAAATTATGTTGAATTCCAAGGGCTACACCAAGTCCATTGATATTTGGT
CTGTAGGCTGCATTCTGGCAGAAATGCTTTCTAACAGGCCCATCTTTCCA
GGGAAGCATTATCTTGACCAGCTGAACCACATTTTGGGTATTCTTGGATC
CCCATCACAAGAAGACCTGAATTGTATAATAAATTTAAAAGCTAGGAACT
ATTTGCTTTCTCTTCCACACAAAAATAAGGTGCCATGGAACAGGCTGTTC
CCAAATGCTGACTCCAAAGCTCTGGACTTATTGGACAAAATGTTGACATT
CAACCCACACAAGAGGATTGAAGTAGAACAGGCTCTGGCCCACCCATATC
TGGAGCAGTATTACGACCCGAGTGACGAGCCCATCGCCGAAGCACCATTC
AAGTTCGACATGGAATTGGATGACTTGCCTAAGGAAAAGCTCAAAGAACT
AATTTTTGAAGAGACTGCTAGATTCCAGCCAGGATACAGATCTTAAGCGG
CCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDAAAAAAGAGPEMV
RGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTY
CQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYK
LLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLK
ICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSV
GCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYL
LSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLE
QYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-3-ERK2140.39 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU650
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag; Calc Mol Weight 68707 Daltons
• Low Temp: No