cDNA Clone MAPKAPK2

• Plasmid: pGEX6P-1-delta 1-45 MAPKAPK2 Myc
• Parent Plasmid: pGEX6P1
• Expressed: GST-delta 1-45 MAPKAPK2 Myc
• Genbank: NM_032960
• Tag: GST
• Species: Human

Sequence of Insert

TTCCACGTCAAGTCCGGCCTGCAGATCAAGAAGAACGCCATCATCGATGA
CTACAAGGTCACCAGCCAGGTCCTGGGGCTGGGCATCAACGGCAAAGTTT
TGCAGATCTTCAACAAGAGGACCCAGGAGAAATTCGCCCTCAAAATGCTT
CAGGACTGCCCCAAGGCCCGCAGGGAGGTGGAGCTGCACTGGCGGGCCTC
CCAGTGCCCCGACATCGTACGGATCGTGGATGTGTACGAGAATCTGTACG
CAGGGAGGAAGTGCCTGCTGATTGTCATGGAATGTTTGGACGGTGGAGAA
CTCTTTAGCCGAATCCAGGATCGAGGAGACCAGGCATTCACAGAAAGAGA
AGCATCCGAAATCATGAAGAGCATCGGTGAGGCCATCCAGTATCTGCATT
CAATCAACATTGCCCATCGGGATGTCAAGCCTGAGAATCTCTTATACACC
TCCAAAAGGCCCAACGCCATCCTGAAACTCACTGACTTTGGCTTTGCCAA
GGAAACCACCAGCCACAACTCTTTGACCACTCCTTGTTATACACCGTACT
ATGTGGCTCCAGAAGTGCTGGGTCCAGAGAAGTATGACAAGTCCTGTGAC
ATGTGGTCCCTGGGTGTCATCATGTACATTCTGCTGTGTGGGTATCCCCC
CTTCTACTCCAACCACGGCCTTGCCATCTCTCCGGGCATGAAGACTCGCA
TCCGAATGGGCCAGTATGAATTTCCCAACCCAGAATGGTCAGAAGTATCA
GAGGAAGTGAAGATGCTCATTCGGAATCTGCTGAAAACAGAGCCCACCCA
GAGAATGACCATCACCGAGTTTATGAACCACCCTTGGATCATGCAATCAA
CAAAGGTCCCTCAAACCCCACTGCACACCAGCCGGGTCCTGAAGGAGGAC
AAGGAGCGGTGGGAGGATGTCAAGGAGGAGATGACCAGTGCCTTGGCCAC
AATGCGCGTTGACTACGAGCAGATCAAGATAAAAAAGATTGAAGATGCAT
CCAACCCTCTGCTGCTGAAGAGGCGGAAGAAAGCTCGGGCCCTGGAGGCT
GCGGCTCTGGGCCACATGGAGCAGAAGCTGATCAGCGAGGAGGACCTGAA
G

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSFPQFHVKSGLQIKKNAIID
DYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRA
SQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFXRIQDRGDQAFTER
EASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFA
KETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYP
PFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPT
QRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALA
TMRVDYEQIKIKKIEDASNPLLLKRRKKARALRAAALGHMEQKLISEEDL
K

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU649
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag