cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pFBHTb TPL2
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-TPL2
• Genbank: P41279
• Tag: 6His
• Species: Human

Sequence of Insert

atggagtacatgagcactggaagtgacaataaagaagagattgatttatt
aattaaacatttaaatgtgtctgatgtaatagacattatggaaaatcttt
atgcaagtgaagagccagcagtttatgaacccagtctaatgaccatgtgt
caagacagtaatcaaaacgatgagcgttctaagtctctgctgcttagtgg
ccaagaggtaccatggttgtcatcagtcagatatggaactgtggaggatt
tgcttgcttttgcaaaccatatatccaacactgcaaagcatttttatgga
caacgaccacaggaatctggaattttattaaacatggtcatcactcccca
aaatggacgttaccaaatagattccgatgttctcctgatcccctggaagc
tgacttacaggaatattggttctgattttattcctcggggcgcctttgga
aaggtatacttggctcaagatataaagacgaagaaaagaatggcgtgtaa
actgatcccagtagatcaatttaagccatctgatgtggaaattcaggctt
gcttccggcacgagaacatcgcagagctgtatggcgcagtcctgtggggt
gaaactgtccatctctttatggaagcaggcgagggagggtctgttctgga
gaaactggagagctgtggaccaatgagagaatttgaaattatttgggtga
caaagcatgttctcaagggacttgattttctacactcaaagaaagtgatc
catcatgatattaaacctagcaacattgttttcatgtccacaaaagctgt
tttggtggattttggcctaagtgttcaaatgaccgaagatgtctattttc
ctaaggacctccgaggaacagagatttacatgagcccagaggtcatcctg
tgcaggggccattcaaccaaagcagacatctacagcctgggggccacgct
catccacatgcagacgggcaccccaccctgggtgaagcgctaccctcgct
cagcctatccctcctacctgtacataatccacaagcaagcacctccactg
gaagacattgcagatgactgcagtccagggatgagagagctgatagaagc
ttccctggagagaaaccccaatcaccgcccaagagccgcagacctactaa
aacatgaggccctgaacccgcccagagaggatcagccacgctgtacgagt
ctggactctgccctcttggagcgcaagaggctgctgagtaggaaggagct
ggaacttcctgagaacattgctgattcttcgtgcacaggaagcaccgagg
aatctgagatgctcaagaggcaacgctctctctacatcgacctcggcgct
ctggctggctacttcaatcttgttcggggaccaccaacgcttgaatatgg
ctga

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSEYMSTGSDNKEEIDLLIKHLNV
SDVIDIMENLYASEEPAVYEPSLMTMCQDSNQNDERSKSLLLSGQEVPWL
SSVRYGTVEDLLAFANHISNTAKHFYGQRPQESGILLNMVITPQNGRYQI
DSDVLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRMACKLIPVDQ
FKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGSVLEKLESCG
PMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGL
SVQMTEDVYFPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTG
TPPWVKRYPRSAYPSYLYIIHKQAPPLEDIADDCSPGMRELIEASLERNP
NHRPRAADLLKHEALNPPREDQPRCTSLDSALLERKRLLSRKELELPENI
ADSSCTGSTEESEMLKRQRSLYIDLGALAGYFNLVRGPPTLEYG*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU648
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag