cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pGEX6P-1-COT
• Parent Plasmid: pGEX6P1
• Expressed: GST-TPL2
• Genbank: P41279
• Tag: GST
• Species: Human

Sequence of Insert

atggagtacatgagcactggaagtgacaataaagaagagattgatttatt
aattaaacatttaaatgtgtctgatgtaatagacattatggaaaatcttt
atgcaagtgaagagccagcagtttatgaacccagtctaatgaccatgtgt
caagacagtaatcaaaacgatgagcgttctaagtctctgctgcttagtgg
ccaagaggtaccatggttgtcatcagtcagatatggaactgtggaggatt
tgcttgcttttgcaaaccatatatccaacactgcaaagcatttttatgga
caacgaccacaggaatctggaattttattaaacatggtcatcactcccca
aaatggacgttaccaaatagattccgatgttctcctgatcccctggaagc
tgacttacaggaatattggttctgattttattcctcggggcgcctttgga
aaggtatacttggctcaagatataaagacgaagaaaagaatggcgtgtaa
actgatcccagtagatcaatttaagccatctgatgtggaaattcaggctt
gcttccggcacgagaacatcgcagagctgtatggcgcagtcctgtggggt
gaaactgtccatctctttatggaagcaggcgagggagggtctgttctgga
gaaactggagagctgtggaccaatgagagaatttgaaattatttgggtga
caaagcatgttctcaagggacttgattttctacactcaaagaaagtgatc
catcatgatattaaacctagcaacattgttttcatgtccacaaaagctgt
tttggtggattttggcctaagtgttcaaatgaccgaagatgtctattttc
ctaaggacctccgaggaacagagatttacatgagcccagaggtcatcctg
tgcaggggccattcaaccaaagcagacatctacagcctgggggccacgct
catccacatgcagacgggcaccccaccctgggtgaagcgctaccctcgct
cagcctatccctcctacctgtacataatccacaagcaagcacctccactg
gaagacattgcagatgactgcagtccagggatgagagagctgatagaagc
ttccctggagagaaaccccaatcaccgcccaagagccgcagacctactaa
aacatgaggccctgaacccgcccagagaggatcagccacgctgtacgagt
ctggactctgccctcttggagcgcaagaggctgctgagtaggaaggagct
ggaacttcctgagaacattgctgattcttcgtgcacaggaagcaccgagg
aatctgagatgctcaagaggcaacgctctctctacatcgacctcggcgct
ctggctggctacttcaatcttgttcggggaccaccaacgcttgaatatgg
ctga

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEYMSTGSDNKEEIDLLIKH
LNVSDVIDIMENLYASEEPAVYEPSLMTMCQDSNQNDERSKSLLLSGQEV
PWLSSVRYGTVEDLLAFANHISNTAKHFYGQRPQESGILLNMVITPQNGR
YQIDSDVLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRMACKLIP
VDQFKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGSVLEKLE
SCGPMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVD
FGLSVQMTEDVYFPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHM
QTGTPPWVKRYPRSAYPSYLYIIHKQAPPLEDIADDCSPGMRELIEASLE
RNPNHRPRAADLLKHEALNPPREDQPRCTSLDSALLERKRLLSRKELELP
ENIADSSCTGSTEESEMLKRQRSLYIDLGALAGYFNLVRGPPTLEYG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU647
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag