cDNA Clone EGFR

• Plasmid: pGEX6P-1-EGFR CAT
• Parent Plasmid: pGEX6P1
• Expressed: GST-EGFR CAT (aa695-1022)
• Genbank: NM_005228
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGA
AACTGAATTCAAAAAGATCAAAGTGCTGGGCTCCGGTGCGTTCGGCACGG
TGTATAAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTC
GCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAAT
CCTCGATGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCC
GCCTGCTGGGCATCTGCCTCACCTCCACCGTGCAGCTCATCACGCAGCTC
ATGCCCTTCGGCTGCCTCCTGGACTATGTCCGGGAACACAAAGACAATAT
TGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGGCATGA
ACTACTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAAC
GTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGC
CAAACTGCTGGGTGCGGAAGAGAAAGAATACCATGCAGAAGGAGGCAAAG
TGCCTATCAAGTGGATGGCATTGGAATCAATTTTACACAGAATCTATACC
CACCAGAGTGATGTCTGGAGCTACGGGGTGACCGTTTGGGAGTTGATGAC
CTTTGGATCCAAGCCATATGACGGAATCCCTGCCAGCGAGATCTCCTCCA
TCCTGGAGAAAGGAGAACGCCTCCCTCAGCCACCCATATGTACCATCGAT
GTCTACATGATCATGGTCAAGTGCTGGATGATAGACGCAGATAGTCGCCC
AAAGTTCCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCC
AGCGCTACCTTGTCATTCAGGGGGATGAAAGAATGCATTTGCCAAGCCCT
ACAGACTCCAACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGA
CGTGGTGGATGCCGACGAGTACCTCATCCCACAGCAGGGCTAAGCGGCCG
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDSGEAPNQALLRIL
KETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANK
EILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKD
NIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFG
LAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWEL
MTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADS
RPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDM
DDVVDADEYLIPQQG

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU605
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion