cDNA Clone EF2 Kinase

• Plasmid: pGEX-FLAG EF2kinase
• Parent Plasmid: pGEX4T1
• Expressed: GST-FLAG EF2kinase
• Genbank: BC032665
• Tag: GST
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGGCAGACGAAGA
CCTCATCTTCCGCCTGGAAGGTGTTGATGGCGGCCAGTCCCCCCGAGCTG
GCCATGATGGTGATTCTGATGGGGACAGCGACGATGAGGAAGGTTACTTC
ATCTGCCCCATCACGGATGACCCAAGCTCGAACCAGAATGTCAATTCCAA
GGTTAATAAGTACTACAGCAACCTAACAAAAAGTGAGCGGTATAGCTCCA
GCGGGTCCCCGGCAAACTCCTTCCACTTCAAGGAAGCCTGGAAGCACGCA
ATCCAGAAGGCCAAGCACATGCCCGACCCCTGGGCTGAGTTCCACCTGGA
AGATATTGCCACCGAACGTGCTACTCGACACAGGTACAACGCCGTCACCG
GGGAATGGCTGGATGATGAAGTTCTGATCAAGATGGCATCTCAGCCCTTC
GGCCGAGGAGCAATGAGGGAGTGCTTCCGGACGAAGAAGCTCTCCAACTT
CTTGCATGCCCAGCAGTGGAAGGGCGCCTCCAACTACGTGGCGAAGCGCT
ACATCGAGCCCGTAGACCGGGATGTGTACTTTGAGGACGTGCGTCTACAG
ATGGAGGCCAAGCTCTGGGGGGAGGAGTATAATCGGCACAAGCCCCCCAA
GCAGGTGGACATCATGCAGATGTGCATCATCGAGCTGAAGGACAGACCGG
GCAAGCCCCTCTTCCACCTGGAGCACTACATCGAGGGCAAGTACATCAAG
TACAACTCCAACTCTGGCTTTGTCCGTGATGACAACATCCGACTGACGCC
GCAGGCCTTCAGCCACTTCACTTTTGAGCGTTCCGGCCATCAGCTGATAG
TGGTGGACATCCAGGGAGTTGGGGATCTCTACACTGACCCACAGATCCAC
ACGGAGACGGGCACTGACTTTGGAGACGGCAACCTAGGTGTCCGCGGGAT
GGCGCTCTTCTTCTACTCTCATGCCTGCAACCGGATTTGCGAGAGCATGG
GCCTTGCTCCCTTTGACCTCTCGCCCCGGGAGAGGGATGCAGTGAATCAG
AACACCAAGCTGCTGCAATCAGCCAAGACCATCTTGAGAGGAACAGAGGA
AAAATGTGGGAGCCCCCGAGTAAGGACCCTCTCTGGGAGCCGGCCACCCC
TGCTCCGTCCCCTTTCAGAGAACTCTGGAGACGAGAACATGAGCGACGTG
ACCTTCGACTCTCTCCCTTCTTCCCCATCTTCGGCCACACCACACAGCCA
GAAGCTAGACCACCTCCATTGGCCAGTGTTCAGTGACCTCGATAACATGG
CATCCAGAGACCATGATCATCTAGACAACCACCGGGAGTCTGAGAATAGT
GGGGACAGCGGATACCCCAGTGAGAAGCGGGGTGAGCTGGATGACCCTGA
GCCCCGAGAACATGGCCACTCATACAGTAATCGGAAGTACGAGTCTGACG
AAGACAGCCTGGGCAGCTCTGGACGGGTATGTGTAGAGAAGTGGAATCTC
CTCAACTCCTCCCGCCTCCACCTGCCGAGGGCTTCGGCCGTGGCCCTGGA
AGTGCAAAGGCTTAATGCTCTGGACCTCGAAAAGAAAATCGGGAAGTCCA
TTTTGGGGAAGGTCCATCTGGCCATGGTGCGCTACCACGAGGGTGGGCGC
TTCTGCGAGAAGGGCGAGGAGTGGGACCAGGAGTCGGCTGTCTTCCACCT
GGAGCACGCAGCCAACCTGGGCGAGCTGGAGGCCATCGTGGGCCTGGGAC
TCATGTACTCGCAGTTGCCTCATCACATCCTAGCCGATGTCTCTCTGAAG
GAGACAGAAGAGAACAAAACCAAAGGATTTGATTACTTACTAAAGGCCGC
TGAAGCTGGCGACAGGCAGTCCATGATCCTAGTGGCGCGAGCTTTTGACT
CTGGCCAGAACCTCAGCCCGGACAGGTGCCAAGACTGGCTAGAGGCCCTG
CACTGGTACAACACTGCCCTGGAGATGACGGACTGTGATGAGGGCGGTGA
GTACGACGGAATGCAGGACGAGCCCCGGTACATGATGCTGGCCAGGGAGG
CAGAGATGCTGTTCACAGGAGGCTACGGGCTGGAGAAGGACCCGCAGAGA
TCAGGGGACTTGTATACCCAGGCAGCAGAGGCAGCGATGGAAGCCATGAA
GGGCCGACTGGCCAACCAGTACTACCAAAAGGCTGAAGAGGCCTGGGCCC
AGATGGAGGAATAAGGTACCAAGGGCGAATTCAGATCTGGTACCACGCGT
ATCGATAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCAGGAAT
TC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSATMDYKDDDDKADEDLIFRLEGVD
GGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLT
KSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATR
HRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGA
SNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCI
IELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFE
RSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHAC
NRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPRVRT
LSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPV
FSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYS
NRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDL
EKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGEL
EAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMI
LVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPR
YMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQ
KAEEAWAQMEE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU596
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for Human EF2Kinase with N terminal GST and FLAG tags