cDNA Clone MEKK2

• Synonyms: MAP3K2
• Plasmid: pGEX-MEKK2 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-MEKK2 6His
• Genbank: AAD28547
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGATGATCAGCAAGCTTTGAACTCAATCATGCAAGATTTGGCTGT
CCTTCATAAGGCCAGTCGACCAGCATTATCCTTGCAGGAAACCAGAAAAG
CAAAATCTTCATCACCAAAAAAACAGAATGATGTCCGAGTCAAATTTGAA
CATAGAGGAGAAAAAAGAATCCTTCAGTTCCCCAGACCAGTTAAACTGGA
AGATCTGAGATCTAAAGCTAAAATTGCCTTTGGACAATCTATGGATCTAC
ATTATACCAATAACGAGTTGGTAATTCCATTAACTACTCAAGATGACTTG
GACAAAGCTGTGGAACTGCTGGATCGTAGTATTCATATGAAGAGCCTCAA
GATATTACTTGTAATAAATGGAAGTACACAGGCTACTAATTTAGAACCAT
TGCCATCACTAGAAGATTTGGATAATACAGTATTTGGAGCAGAGAGGAAA
AAACGGCTATCTATAATAGGTCCTACTAGTAGAGATAGAAGTTCTCCTCC
CCCAGGTTACATTCCAGATGAATTACACCAGGTTGCCCGGAATGGGTCAT
TCACTAGTATCAACAGTGAAGGAGAGTTCATTCCAGAGAGCATGGACCAA
ATGCTGGATCCATTATCTTTAAGCAGCCCTGAAAATTCTGGCTCAGGAAG
TTGTCCATCACTTGATAGTCCTTTGGATGGAGAGAGCTATCCAAAATCAC
GAATGCCTAGGGCTCAGAGCTACCCAGATAATCATCAGGAATTTTCAGAC
TATGATAACCCTATCTTTGAGAAATTTGGAAAAGGAGGAACATATCCAAG
AAGGTATCATGTTTCATATCATCATCAAGAGTATAATGATGGTCGTAAAA
CTTTTCCAAGAGCCAGAAGGACCCAGGGGACCAGCTTACGGTCTCCTGTG
AGTTTCAGTCCTACTGATCATTCCTTAAGCACTAGTAGTGGAAGCAGTAT
CTTTACCCCAGAGTATGATGATAGTCGAATAAGAAGAAGGGGAAGTGACA
TAGACAATCCTACTTTGACCGTAATGGACATCAGCCCACCCAGCCGTTCA
CCTCGAGCTCCGACCAACTGGAGATTGGGCAAACTGCTTGGCCAAGGAGC
CTTTGGAAGGGTCTACCTCTGTTATGATGTTGATACAGGAAGAGAATTGG
CTGTTAAGCAAGTTCAATTTGACCCCGATAGTCCTGAGACCAGCAAGGAA
GTAAATGCACTTGAGTGTGAAATTCAGTTGCTGAAAAACTTGCTACATGA
GCGAATTGTTCAGTATTATGGCTGTTTGAGGGATCCCCAGGAAAAAACAC
TTTCCATATTTATGGAATATATGCCAGGGGGTTCAATTAAGGACCAATTA
AAAGCATATGGCGCTCTTACTGAGAATGTGACTAGGAAATACACCCGTCA
GATTCTGGAGGGTGTCCATTATTTGCACAGTAATATGATTGTCCATAGAG
ATATCAAAGGCGCAAATATCCTGCGAGATTCAACAGGCAACGTCAAACTA
GGAGATTTTGGGGCCAGCAAACGGCTTCAGACCATCTGTCTCTCAGGGAC
AGGAATGAAGTCTGTCACGGGCACACCATACTGGATGAGCCCTGAAGTCA
TCAGTGGAGAAGGCTATGGAAGAAAAGCAGACATCTGGAGTGTTGCATGT
ACTGTGGTAGAAATGCTAACTGAAAAGCCGCCTTGGGCTGAATTTGAAGC
AATGGCTGCCATCTTTAAAATCGCCACTCAGCCAACAAACCCAAAGCTGC
CACCTCATGTCTCAGACTATACTCGAGATTTCCTCAAACGGATTTTTGTA
GAGGCCAAACTGAGACCTTCAGCTGATGAACTCTTAAGGCACATGTTTGT
GCATTATCACCACCATCACCATCACCATGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFDDQQALNSIMQDLAVL
HKASRPALSLQETRKAKSSSPKKQNDVRVKFEHRGEKRILQFPRPVKLED
LRSKAKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKI
LLVINGSTQATNLEPLPSLEDLDNTVFGAERKKRLSIIGPTSRDRSSPPP
GYIPDELHQVARNGSFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSC
PSLDSPLDGESYPKSRMPRAQSYPDNHQEFSDYDNPIFEKFGKGGTYPRR
YHVSYHHQEYNDGRKTFPRARRTQGTSLRSPVSFSPTDHSLSTSSGSSIF
TPEYDDSRIRRRGSDIDNPTLTVMDISPPSRSPRAPTNWRLGKLLGQGAF
GRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLKNLLHER
IVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQI
LEGVHYLHSNMIVHRDIKGANILRDSTGNVKLGDFGASKRLQTICLSGTG
MKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAM
AAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSADELLRHMFVH
YHHHHHHAAAS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU585
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST and C terminal 6His tags. MEKK2 was RT from HELA extract. Differs to the noted Genbank sequence L105V, E200D, G227D, K277Q, D278E, 279+Y, N294T, Q295S, T297R, F414L, G460V, L481V, Q537E