cDNA Clone CDC7

• Plasmid: pFB HTc CDC7
• Parent Plasmid: pFastbac HTc
• Expressed: 6His-HTc CDC7
• Genbank: AB003698
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGGGATCCGGAATTCAAAGGCCT
ACGTCGACGAGGCGTCTTTGGGGATTCAGATGGATGAGCCAATGGCTTTT
TCTCCCCAGCGTGACCGGTTTCAGGCTGAAGGCTCTTTAAAAAAAAACGA
GCAGAATTTTAAACTTGCAGGTGTTAAAAAAGATATTGAGAAGCTTTATG
AAGCTGTACCACAGCTTAGTAATGTGTTTAAGATTGAGGACAAAATTGGA
GAAGGCACTTTCAGCTCTGTTTATTTGGCCACAGCACAGTTACAAGTAGG
ACCTGAAGAGAAAATTGCTCTAAAACACTTGATTCCAACAAGTCATCCTA
TAAGAATTGCAGCTGAACTTCAGTGCCTAACAGTGGCTGGGGGGCAAGAT
AATGTCATGGGAGTTAAATACTGCTTTAGGAAGAATGATCATGTAGTTAT
TGCTATGCCATATCTGGAGCATGAGTCGTTTTTGGACATTCTGAATTCTC
TTTCCTTTCAAGAAGTACGGGAATATATGCTTAATCTGTTCAAAGCTTTG
AAACGCATTCATCAGTTTGGTATTGTTCACCGTGATGTTAAGCCCAGCAA
TTTTTTATATAATAGGCGCCTGAAAAAGTATGCCTTGGTAGACTTTGGTT
TGGCCCAAGGAACCCATGATACGAAAATAGAGCTTCTTAAATTTGTCCAG
TCTGAAGCTCAGCAGGAAAGGTGTTCACAAAACAAATCCCACATAATCAC
AGGAAACAAGATTCCACTGAGTGGCCCAGTACCTAAGGAGCTGGATCAGC
AGTCCACCACAAAAGCTTCTGTTAAAAGACCCTACACAAATGCACAAATT
CAGATTAAACAAGGAAAAGACGGAAAGGAGGGATCTGTAGGCCTTTCTGT
CCAGCGCTCTGTTTTTGGAGAAAGAAATTTCAATATACACAGCTCCATTT
CACATGAGAGCCCTGCAGTGAAACTCATGAAGCAGTCAAAGACTGTGGAT
GTACTGTCTAGAAAGTTAGCAACAAAAAAGAAGGCTATTTCTACGAAAGT
TATGAATAGTGCTGTGATGAGGAAAACTGCCAGTTCTTGCCCAGCTAGCC
TGACCTGTGACTGCTATGCAACAGATAAAGTTTGTAGTATTTGCCTTTCA
AGGCGTCAGCAGGTTGCCCCTAGGGCAGGTACACCAGGATTCAGAGCACC
AGAGGTCTTGACAAAGTGCCCCAATCAAACTACAGCAATTGACATGTGGT
CTGCAGGTGTCATATTTCTTTCTTTGCTTAGTGGACGATATCCATTTTAT
AAAGCAAGTGATGATTTAACTGCTTTGGCCCAAATTATGACAATTAGGGG
ATCCAGAGAAACTATCCAAGCTGCTAAAACTTTTGGGAAATCAATATTAT
GTAGCAAAGAAGTTCCAGCACAAGACTTGAGAAAACTCTGTGAGAGACTC
AGGGGTATGGATTCTAGCACTCCCAAGTTAACAAGTGATATACAAGGGCA
TGCTTCTCATCAACCAGCTATTTCAGAGAAGACTGACCATAAAGCTTCTT
GCCTCGTTCAAACACCTCCAGGACAATACTCAGGGAATTCATTTAAAAAG
GGGGATAGTAATAGCTGTGAGCATTGTTTTGATGAGTATAATACCAATTT
AGAAGGCTGGAATGAGGTACCTGATGAAGCTTATGACCTGCTTGATAAAC
TTCTAGATCTAAATCCAGCTTCAAGAATAACAGCAGAAGAAGCTTTGTTG
CATCCATTTTTTAAAGATATGAGCTTGTGA

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDEASLGIQMDEPMAF
SPQRDRFQAEGSLKKNEQNFKLAGVKKDIEKLYEAVPQLSNVFKIEDKIG
EGTFSSVYLATAQLQVGPEEKIALKHLIPTSHPIRIAAELQCLTVAGGQD
NVMGVKYCFRKNDHVVIAMPYLEHESFLDILNSLSFQEVREYMLNLFKAL
KRIHQFGIVHRDVKPSNFLYNRRLKKYALVDFGLAQGTHDTKIELLKFVQ
SEAQQERCSQNKSHIITGNKIPLSGPVPKELDQQSTTKASVKRPYTNAQI
QIKQGKDGKEGSVGLSVQRSVFGERNFNIHSSISHESPAVKLMKQSKTVD
VLSRKLATKKKAISTKVMNSAVMRKTASSCPASLTCDCYATDKVCSICLS
RRQQVAPRAGTPGFRAPEVLTKCPNQTTAIDMWSAGVIFLSLLSGRYPFY
KASDDLTALAQIMTIRGSRETIQAAKTFGKSILCSKEVPAQDLRKLCERL
RGMDSSTPKLTSDIQGHASHQPAISEKTDHKASCLVQTPPGQYSGNSFKK
GDSNSCEHCFDEYNTNLEGWNEVPDEAYDLLDKLLDLNPASRITAEEALL
HPFFKDMSL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU584
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag