cDNA Clone PNGase (mouse)

• Plasmid: pCMV-FLAG PNGase
• Parent Plasmid: pCMV5
• Expressed: FLAG-PNGase
• Genbank: AF250927
• Tag: FLAG
• Species: Mouse

Sequence of Insert

GCGAATTCGCCACCATGGACTACAAGGACGACGATGACAAGGCGTCGGCC
ACACTGGGCAGCTCGTCGAGCTCTGCGTCCCCGGCCGTGGCCGAGCTATG
CCAGAACACCCCGGAGACCTTTCTGGAGGCCTCCAAGCTGCTGCTCACCT
ACGCCGACAACATCCTGAGAAACCCCAGTGATGAAAAATACAGATCCATC
CGTATTGGGAACACAGCGTTTTCTACTAGACTCTTGCCTGTCAGAGGAGC
TGTTGAGTGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACGCATCTTA
TCTTTCCTAAAAAAGCTTCAGTGGAACAGCTACAGAAAATCCGTGATCTG
ATTGCCATAGAGAGAAGCAGCAGACTGGACGGATCCAGTAAGAAGGTGCA
ATTCTCTCAGCACCCGGCTGCTGCCAAGCTTCCTCTGGAGCAGTCTGAAG
ATCCTGCTGGGTTAATACGGCACTCAGGAAACCAAACAGGGCAGCTGCCA
AGCCTACCCTCTGCTCCAATGGTTGTTGGTGATTCAACCATCTTAAAAGT
TCTTCAGTCCAACATTCAGCATGTGCAGCTCTATGAAAACCCTGTCCTCC
AGGAGAAAGCATTGACTTGTATCCCAGTCAGTGAACTAAAAAGAAAAGCT
CAAGAAAAGTTATTCAGAGCTAGAAAGTTAGATAAAGGTACTAATGTAAG
TGATGAGGACTTCCTTCTACTGGAGCTCCTACACTGGTTTAAAGAGGAGT
TTTTTCGCTGGGTGAATAACATTGTGTGCAGCAAATGTGGTGGAGAGACT
AGATCTAGAGATGAGGCATTGCTGCCCAATGATGATGAACTGAAGTGGGG
TGCAAAGAATGTGGAGAATCATTACTGTGATGCCTGCCAGTTGAGCAACC
GCTTCCCAAGATATAACAACCCCGAGAAACTTTTGGAAACAAGATGTGGA
CGCTGTGGTGAATGGGCCAATTGTTTTACTCTGTGCTGCCGTGCATTAGG
GTTTGAAGCTCGATATGTGTGGGATTACACAGACCATGTTTGGACAGAAG
TCTATTCTCCATCTCAGCAGCGGTGGCTACACTGTGATGCATGTGAGGAT
GTCTGTGACAAACCCCTCCTTTATGAAATAGGATGGGGCAAGAAGCTTTC
CTATATAATAGCATTCTCTAAAGATGAGGTAGTTGATGTCACCTGGCGAT
ACTCTTGTAAACATGATGAGGTGATGTCCAGGAGGACCAAGGTTAAAGAA
GAGTTACTTCGAGAAACTATTAATGGGCTTAATAAGCAGAGACAGCTATC
ATTATCAGAAAGCAGAAGGAAAGAACTTCTCCAGAGGATAATTGTGGAGC
TTGTTGAATTTATATCTCCTAAAACCCCAAGACCTGGAGAACTTGGTGGA
AGGGTATCCGGGTCATTGGCTTGGAGAGTGGCCCGAGGTGAGACGGGTCT
AGAGAGAAAGGAGATTCTGTTCATCCCTTCTGAAAATGAAAAGATTTCTA
AGCAGTTTCACCTCCGTTATGATATTGTGAGAGACCGTTACATTCGTGTC
TCAGACAACAACACAAACATTTCTGGATGGGAGAATGGTGTGTGGAAAAT
GGAATCCATATTCAGAAAAGTCGAGAAAGACTGGAACATGGTTTATTTGG
CCCGAAAAGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTTTGAATGT
GGGTCAGCTGGTCTAAAAGTAGATACTGTTTCCATCCGCACAAGTAGCCA
AAGCTTTGAGTCTGGATCAGTGAGGTGGAAACTGCGATCTGAGACAGCTC
AAGTCAACCTGCTGGGAGATAAAAATCTACGTTCCTATAATGATTTTTCT
GGTGCCACAGAGGTTACATTAGAAGCAGAGTTAAGCAGAGGAGATGGAGA
CGTTGCTTGGCAACATACCCAACTGTTTAGACAAAGCTTAAATGACAGTG
GAGAAAATGGCTTGGAAATAATTATAACGTTCAATGACCTCTGAGTCGAC

Amino Acid Sequence

MDYKDDDDKASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNI
LRNPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKK
ASVEQLQKIRDLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPAGL
IRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHVQLYENPVLQEKAL
TCIPVSELKRKAQEKLFRARKLDKGTNVSDEDFLLLELLHWFKEEFFRWV
NNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYCDACQLSNRFPRY
NNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPS
QQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKH
DEVMSRRTKVKEELLRETINGLNKQRQLSLSESRRKELLQRIIVELVEFI
SPKTPRPGELGGRVSGSLAWRVARGETGLERKEILFIPSENEKISKQFHL
RYDIVRDRYIRVSDNNTNISGWENGVWKMESIFRKVEKDWNMVYLARKEG
SSFAYISWKFECGSAGLKVDTVSIRTSSQSFESGSVRWKLRSETAQVNLL
GDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDSGENGL
EIIITFNDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU577
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian construct to express FLAG N terminal fusion