cDNA Clone GSK3 beta

• Synonyms: GSK3B
• Plasmid: pEBG-2T-GSK3 wt
• Parent Plasmid: pEBG2T
• Expressed: GST-GSK3 wt
• Genbank: L33801
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTGCCACCATGGAGTTCATGCCCATGGAGTCAGGGCGGCCCAGAAC
CACCTCCTTTGCGGAGAGCTGCAAGCCGGTGCAGCAGCCTTCAGCTTTTG
GCAGCATGAAAGTTAGCAGAGACAAGGACGGCAGCAAGGTGACAACAGTG
GTGGCAACTCCTGGGCAGGGTCCAGACAGGCCACAAGAAGTCAGCTATAC
AGACACTAAAGTGATTGGAAATGGATCATTTGGTGTGGTATATCAAGCCA
AACTTTGTGATTCAGGAGAACTGGTCGCCATCAAGAAAGTATTGCAGGAC
AAGAGATTTAAGAATCGAGAGCTCCAGATCATGAGAAAGCTAGATCACTG
TAACATAGTCCGATTGCGTTATTTCTTCTACTCCAGTGGTGAGAAGAAAG
ATGAGGTCTATCTTAATCTGGTGCTGGACTATGTTCCGGAAACAGTATAC
AGAGTTGCCAGACACTATAGTCGAGCCAAACAGACGCTCCCTGTGATTTA
TGTCAAGTTGTATATGTATCAGCTGTTCCGAAGTTTAGCCTATATCCATT
CCTTTGGAATCTGCCATCGGGATATTAAACCGCAGAACCTCTTGTTGGAT
CCTGATACTGCTGTATTAAAACTCTGTGACTTTGGAAGTGCAAAGCAGCT
GGTCCGAGGAGAACCCAATGTTTCGTATATCTGTTCTCGGTACTATAGGG
CACCAGAGTTGATCTTTGGAGCCACTGATTATACCTCTAGTATAGATGTA
TGGTCTGCTGGCTGTGTGTTGGCTGAGCTGTTACTAGGACAACCAATATT
TCCAGGGGATAGTGGTGTGGATCAGTTGGTAGAAATAATCAAGGTCCTGG
GAACTCCAACAAGGGAGCAAATCAGAGAAATGAACCCAAACTACACAGAA
TTTAAATTCCCTCAAATTAAGGCACATCCTTGGACTAAGGTCTTCCGACC
CCGAACTCCACCGGAGGCAATTGCACTGTGTAGCCGTCTGCTGGAGTATA
CACCAACTGCCCGACTAACACCACTGGAAGCTTGTGCACATTCATTTTTT
GATGAATTACGGGACCCAAATGTCAAACTACCAAATGGGCGAGACACACC
TGCACTCTTCAACTTCACCACTCAAGAACTGTCAAGTAATCCACCTCTGG
CTACCATCCTTATTCCTCCTCATGCTCGGATTCAAGCAGCTGCTTCAACC
CCCACAAATGCCACAGCAGCGTCAGATGCTAATACTGGAGACCGTGGACA
GACCAATAATGCTGCTTCTGCATCAGCTTCCAACTCCACCTGAACAGTCC
CGAGCAGCCAGCTGCACAGGAAAAACCACCAGTTACTTGAGTGTCACTCA
GCAACACTGGTCACGTTTGGAAAGAATATTAAAAAAAAAAAAAAAAGGAA
TTCGATATCAAGCTTATCGATGCGGCCGCG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSATMEFMPMESGRPRTTSFAESCKP
VQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGS
FGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFF
YSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLF
RSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSY
ICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQL
VEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIAL
CSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQE
LSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASA
SNST*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU517
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/EE tag for mammalian expression