cDNA Clone MEKK1 (mouse)

• Synonyms: Map3k1
• Plasmid: pGEX-MEKK1 6His
• Parent Plasmid: pGEX6P1
• Expressed: GST-MEKK1 6His
• Genbank: L13103
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCGTGACCGCAGTGCCCGCTGTGTTTTCCAAGCTGGTAACCATGCT
TAATGCTTCTGGCTCCACCCACTTCACCAGGATGCGCCGGCGTCTGATGG
CTATCGCGGATGAGGTAGAAATTGCCGAGGTCATCCAGCTGGGTGTGGAG
GACACTGTGGATGGGCATCAGGACAGCTTACAGGCCGTGGCCCCCACCAG
CTGTCTAGAAAACAGCTCCCTTGAGCACACAGTCCATAGAGAGAAAACTG
GAAAAGGACTAAGTGCTACGAGACTGAGTGCCAGCTCGGAGGACATTTCT
GACAGACTGGCCGGCGTCTCTGTAGGACTTCCCAGCTCAACAACAACAGA
ACAACCAAAGCCAGCGGTTCAAACAAAAGGCAGACCCCACAGTCAGTGTT
TGAACTCCTCCCCTTTGTCTCATGCTCAATTAATGTTCCCAGCACCATCA
GCCCCTTGTTCCTCTGCCCCGTCTGTCCCAGATATTTCTAAGCACAGACC
CCAGGCATTTGTTCCCTGCAAAATACCTTCCGCATCTCCTCAGACACAGC
GCAAGTTCTCTCTACAATTCCAGAGGAACTGCTCTGAACACCGAGACTCA
GACCAGCTCTCCCCAGTCTTCACTCAGTCAAGACCCCCACCCTCCAGTAA
CATACACAGGCCAAAGCCATCCCGACCCGTTCCGGGCAGTACAAGCAAAC
TAGGGGACGCCACAAAAAGTAGCATGACACTTGATCTGGGCAGTGCTTCC
AGGTGTGACGACAGCTTTGGCGGCGGCGGCAACAGTGGCAACGCCGTCAT
ACCCAGCGACGAGACAGTGTTCACGCCGGTGGAGGACAAGTGCAGGTTAG
ATGTGAACACCGAGCTCAACTCCAGCATCGAGGACCTTCTTGAAGCATCC
ATGCCTTCAAGTGACACGACAGTCACTTTCAAGTCCGAAGTCGCCGTCCT
CTCTCCGGAAAAGGCCGAAAATGACGACACCTACAAAGACGACGTCAATC
ATAATCAAAAGTGCAAAGAAAAGATGGAAGCTGAAGAGGAGGAGGCTTTA
GCGATCGCCATGGCGATGTCAGCGTCTCAGGATGCCCTCCCCATCGTCCC
TCAGCTGCAGGTGGAAAATGGAGAAGATATTATCATCATTCAGCAGGACA
CACCAGAAACTCTTCCAGGACATACCAAAGCGAAACAGCCTTACAGAGAA
GACGCTGAGTGGCTGAAAGGCCAGCAGATAGGCCTCGGAGCATTTTCTTC
CTGTTACCAAGCACAGGATGTGGGGACTGGGACTTTAATGGCTGTGAAAC
AGGTGACGTACGTCAGAAACACATCCTCCGAGCAGGAGGAGGTGGTGGAA
GCGTTGAGGGAAGAGATCCGGATGATGGGTCACCTCAACCATCCAAACAT
CATCCGGATGCTGGGGGCCACGTGCGAGAAGAGCAACTACAACCTCTTCA
TTGAGTGGATGGCGGGAGGATCTGTGGCTCACCTCTTGAGTAAATACGGA
GCTTTCAAGGAGTCAGTCGTCATTAACTACACTGAGCAGTTACTGCGTGG
CCTTTCCTATCTCCACGAGAACCAGATCATTCACAGAGACGTCAAAGGTG
CCAACCTGCTCATTGACAGCACCGGTCAGAGGCTGAGAATTGCAGACTTT
GGAGCTGCTGCCAGGTTGGCATCAAAAGGAACCGGTGCAGGAGAGTTCCA
GGGACAGTTACTGGGGACAATTGCATTCATGGCGCCTGAGGTCCTAAGAG
GTCAGCAGTATGGTAGGAGCTGTGATGTATGGAGTGTTGGCTGCGCCATT
ATAGAAATGGCTTGTGCAAAACCACCTTGGAATGCAGAAAAACACTCCAA
TCATCTCGCCTTGATATTTAAGATTGCTAGCGCAACTACTGCACCGTCCA
TCCCGTCACACCTGTCCCCGGGTCTGCGCGACGTGGCCGTGCGCTGCTTA
GAACTTCAGCCTCAGGACCGGCCTCCGTCCAGAGAGCTGCTGAAACATCC
GGTCTTCCGTACCACGCACCATCACCATCACCATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSVTAVPAVFSKLVTMLNASG
STHFTRMRRRLMAIADEVEIAEVIQLGVEDTVDGHQDSLQAVAPTSCLEN
SSLEHTVHREKTGKGLSATRLSASSEDISDRLAGVSVGLPSSTTTEQPKP
AVQTKGRPHSQCLNSSPLSHAQLMFPAPSAPCSSAPSVPDISKHRPQAFV
PCKIPSASPQTQRKFSLQFQRNCSEHRDSDQLSPVFTQSRPPPSSNIHRP
KPSRPVPGSTSKLGDATKSSMTLDLGSASRCDDSFGGGGNSGNAVIPSDE
TVFTPVEDKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEK
AENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQV
ENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWLKGQQIGLGAFSSCYQA
QDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRML
GATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYL
HENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLL
GTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLAL
IFKIASATTAPSIPSHLSPGLRDVAVRCLELQPQDRPPSRELLKHPVFRT
THHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU480
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal and 6His C terminal fusion