cDNA Clone SGK3

• Plasmid: pETBlue 6His SGK3
• Parent Plasmid: pETBlue2 original
• Expressed: 6His SGK3
• Genbank: BC015326
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccccaccatcaccatcaccatctggaagttctgttccaggggcccc
aaagagatcacaccatggactacaaggaaagctgcccaagtgtaagcatt
cccagctccgatgaacacagagagaaaaagaagaggtttactgtttataa
agttctggtttcagtgggaagaagtgaatggtttgtcttcaggagatatg
cagagtttgataaactttataacactttaaaaaaacagtttcctgctatg
gccctgaagattcctgccaagagaatatttggtgataattttgatccaga
ttttattaaacaaagacgagcaggactaaacgaattcattcagaacctag
ttaggtatccagaactttataaccatccagatgtcagagcattccttcaa
atggacagtccaaaacaccagtcagatccatctgaagatgaggatgaaag
aagttctcagaagctacactctacctcacagaacatcaacctgggaccgt
ctggaaatcctcatgccaaaccaactgactttgatttcttaaaagttatt
ggaaaaggcagctttggcaaggttcttcttgcaaaacggaaactggatgg
aaaattttatgctgtcaaagtgttacagaaaaaaatagttctcaacagaa
aagagcaaaaacatattatggctgaacgtaatgtgctcttgaaaaatgtg
aaacatccgtttttggttggattgcattattccttccaaacaactgaaaa
gctttattttgttctggattttgttaatggaggggagctttttttccact
tacaaagagaacggtcctttcctgagcacagagctaggttttacgctgct
gaaattgctagtgcattgggttacttacattccatcaaaatagtatacag
agacttgaaaccagaaaatattcttttggattcagtaggacatgttgtct
taacagattttgggctttgtaaagaaggaattgctatttctgacaccact
accacattttgtgggacaccagagtatcttgcacctgaagtaattagaaa
acagccctatgacaatactgtagattggtggtgccttggggctgttctgt
atgaaatgctgtatggattgcctcctttttattgccgagatgttgctgaa
atgtatgacaatatccttcacaaacccctaagtttgaggccaggagtgag
tcttacagcctggtccattctggaagaactcctagaaaaagacaggcaaa
atcgacttggtgccaaggaagactttcttgaaattcagaatcatcctttt
tttgaatcactcagctgggctgaccttgtacaaaagaagattccaccacc
atttaatcctaatgtggctggaccagatgatatcagaaactttgacacag
catttacagaagaaacagttccatattctgtgtgtgtatcttctgactat
tctatagtgaatgccagtgtattggaggcagatgatgcattcgttggttt
ctcttatgcacctccttcagaagacttatttttgtgaggatcc

Amino Acid Sequence

HHHHHHLEVLFQGPQRDHTMDYKESCPSVSIPSSDEHREKKKRFTVYKVL
VSVGRSEWFVFRRYAEFDKLYNTLKKQFPAMALKIPAKRIFGDNFDPDFI
KQRRAGLNEFIQNLVRYPELYNHPDVRAFLQMDSPKHQSDPSEDEDERSS
QKLHSTSQNINLGPSGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKF
YAVKVLQKKIVLNRKEQKHIMAERNVLLKNVKHPFLVGLHYSFQTTEKLY
FVLDFVNGGELFFHLQRERSFPEHRARFYAAEIASALGYLHSIKIVYRDL
KPENILLDSVGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQP
YDNTVDWWCLGAVLYEMLYGLPPFYCRDVAEMYDNILHKPLSLRPGVSLT
AWSILEELLEKDRQNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFN
PNVAGPDDIRNFDTAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSY
APPSEDLFL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU451
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express N terminal fusion.