cDNA Clone GSK3 beta

• Synonyms: GSK3B
• Plasmid: pGEX-GSK3 beta K85A
• Parent Plasmid: pGEX6P1
• Expressed: GST-GSK3 beta K85A
• Genbank: L33801
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTGCCACCATGGAGTTCATGCCCATGGAGTCAGGGCGGCCCAGAAC
CACCTCCTTTGCGGAGAGCTGCAAGCCGGTGCAGCAGCCTTCAGCTTTTG
GCAGCATGAAAGTTAGCAGAGACAAGGACGGCAGCAAGGTGACAACAGTG
GTGGCAACTCCTGGGCAGGGTCCAGACAGGCCACAAGAAGTCAGCTATAC
AGACACTAAAGTGATTGGAAATGGATCATTTGGTGTGGTATATCAAGCCA
AACTTTGTGATTCAGGAGAACTGGTCGCCATCGCGAAAGTATTGCAGGAC
AAGAGATTTAAGAATCGAGAGCTCCAGATCATGAGAAAGCTAGATCACTG
TAACATAGTCCGATTGCGTTATTTCTTCTACTCCAGTGGTGAGAAGAAAG
ATGAGGTCTATCTTAATCTGGTGCTGGACTATGTTCCGGAAACAGTATAC
AGAGTTGCCAGACACTATAGTCGAGCCAAACAGACGCTCCCTGTGATTTA
TGTCAAGTTGTATATGTATCAGCTGTTCCGAAGTTTAGCCTATATCCATT
CCTTTGGAATCTGCCATCGGGATATTAAACCGCAGAACCTCTTGTTGGAT
CCTGATACTGCTGTATTAAAACTCTGTGACTTTGGAAGTGCAAAGCAGCT
GGTCCGAGGAGAACCCAATGTTTCGTATATCTGTTCTCGGTACTATAGGG
CACCAGAGTTGATCTTTGGAGCCACTGATTATACCTCTAGTATAGATGTA
TGGTCTGCTGGCTGTGTGTTGGCTGAGCTGTTACTAGGACAACCAATATT
TCCAGGGGATAGTGGTGTGGATCAGTTGGTAGAAATAATCAAGGTCCTGG
GAACTCCAACAAGGGAGCAAATCAGAGAAATGAACCCAAACTACACAGAA
TTTAAATTCCCTCAAATTAAGGCACATCCTTGGACTAAGGTCTTCCGACC
CCGAACTCCACCGGAGGCAATTGCACTGTGTAGCCGTCTGCTGGAGTATA
CACCAACTGCCCGACTAACACCACTGGAAGCTTGTGCACATTCATTTTTT
GATGAATTACGGGACCCAAATGTCAAACTACCAAATGGGCGAGACACACC
TGCACTCTTCAACTTCACCACTCAAGAACTGTCAAGTAATCCACCTCTGG
CTACCATCCTTATTCCTCCTCATGCTCGGATTCAAGCAGCTGCTTCAACC
CCCACAAATGCCACAGCAGCGTCAGATGCTAATACTGGAGACCGTGGACA
GACCAATAATGCTGCTTCTGCATCAGCTTCCAACTCCACCTGA

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMEFMPMESGRPRTTSFA
ESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKV
IGNGSFGVVYQAKLCDSGELVAIAKVLQDKRFKNRELQIMRKLDHCNIVR
LRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLY
MYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGE
PNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDS
GVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPP
EAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFN
FTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNA
ASASASNST*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU430
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag.