cDNA Clone SAP97 (rat)

• Plasmid: pGEX-2T SAP97
• Parent Plasmid: pGEX2T
• Expressed: GST-SAP97
• Genbank: U14950
• Tag: GST
• Species: Rat

Sequence of Insert

GGATCCATGCCGGTCCGGAAGCAAGATACCCAGAGAGCATTGCATCTGTT
GGAAGAATATCGGTCGAAACTAAACCAAACCGAAGACAGACAGCTCAGGA
GTTCCATAGAGCGGGTTATTAGCATATTTCAGAGCAACCTCTTTCAGGCT
TTAATAGACATTCAAGAATTTTATGAAGTGACCTTACTTGATAATCCAAA
ATGTGTGGATCATTCAAAGCAGTGTGAACCGGTCCAACCCGGGAATCCTT
GGGAGAGTGGCAGCCTTTCAAGTGCTGCTGTGACTTCAGAGAGCCTGCCC
GGCGGCCTTAGCCCTCCAGTAGAGAAATACCGGTATCAGGACGAAGAGGT
GCTGCCTTCAGAGCGGATTTCCCCGCAAGTCCCAAATGAGGTGCTGGGTC
CGGAGCTGGTTCACGTCTCGGAGAAGAGCCTGTCAGAGATTGAGAATGTC
CACGGGTTTGTCTCTCACTCTCATATCTCACCCATAAAGGCAAATCCTCC
TCCTGTGCTGGTCAACACAGACAGCTTGGAGACACCAACTTATGTTAATG
GTACTGATGCAGATTATGAATATGAGGAAATCACACTTGAAAGGGGAAAT
TCAGGCCTTGGTTTCAGCATTGCAGGAGGTACAGACAACCCACACATTGG
AGATGACTCAAGTATTTTCATCACCAAAATTATCACGGGAGGAGCAGCTG
CCCAGGATGGAAGATTGCGGGTAAATGACTGTATACTGAGAGTAAATGAA
GCAGATGTTCGTGATGTAACCCACAGCAAAGCAGTTGAAGCATTGAAAGA
AGCTGGATCGATAGTGCGATTATATGTGAAAAGGCGGAAGCCAGCCTCAG
AAAAAATCATGGAAATAAAACTTATTAAAGGCCCTAAAGGTCTTGGGTTC
AGCATTGCTGGAGGTGTTGGAAACCAGCACATTCCTGGGGATAACAGCAT
CTATGTAACCAAAATAATTGAAGGAGGTGCAGCACACAAGGACGGCAAAC
TTCAGATTGGAGACAAGCTTCTAGCAGTGAACAGTGTCTGTTTAGAAGAA
GTTACTCATGAAGAAGCAGTGACTGCCTTAAAGAATACATCTGATTTTGT
TTATTTGAAAGTGGCAAAACCAACAAGTATGTATATAAATGATGGCTATG
CACCACCCGACATCACTAATTCTTCTTCTCAGTCTGTTGACAACCATGTT
AGCCCGTCTTCATACTTGGGCCAGACTCCAGCGTCACCAGCCAGATACTC
ACCCATTTCTAAAGCAGTGCTTGGAGATGATGAGATCACTAGGGAACCTA
GAAAAGTTGTTCTTCATCGTGGCTCAACGGGACTTGGTTTCAACATTGTG
GGAGGCGAAGATGGAGAAGGGATTTTTATCTCTTTCATCCTTGCTGGGGG
ACCTGCTGACCTAAGCGGAGAGCTCAGAAAAGGAGATCGCATTATATCGG
TAAACAGTGTTGACCTCAGAGCTGCAAGTCATGAACAAGCAGCTGCCGCA
TTAAAAAACGCAGGCCAAGCTGTCACTATTGTCGCACAGTATCGGCCTGA
AGAGTACAGTCGTTTTGAAGCTAAAATTCACGACTTGCGGGAGCAGATGA
TGAACAGCAGTGTCAGCTCAGGGTCAGGGTCTCTCCGAACCAGCCAAAAG
CGGTCCCTCTATGTCAGAGCCCTTTTTGATTACGACAAGACTAAAGACAG
TGGCCTTCCCAGTCAAGGGTTGAACTTCAAATTCGGAGATATTCTCCATG
TTATCAATGCTTCTGATGACGAGTGGTGGCAGGCCAGACAGGTGACCCCA
GATGGTGAGAGTGACGAAGTTGGAGTAATTCCTAGTAAACGCAGAGTTGA
GAAGAAAGAACGAGCCCGATTAAAAACAGTCAAATTCAATTCTAAAACAA
GAGGAGATAAAGGGTCATTCAATGACAAGCGTAAAAAGAACCTCTTTTCC
CGAAAATTTCCCTTCTACAAGAACAAGGACCAGAGTGAGCAGGAAACAAG
TGATGCTGACCAGCACGTAACTTCTAATGCCAGCGATAGCGAAAGTAGTT
ACCGTGGTCAAGAAGAATATGTTTTATCGTATGAGCCGGTGAATCAGCAA
GAAGTTAATTATACCCGACCAGTCATCATATTAGGACCTATGAAAGACAG
AGTAAATGATGACTTAATCTCAGAATTTCCTGACAAATTTGGATCCTGTG
TCCCTCATACAACTAGACCAAAGCGTGACTATGAGGTGGATGGACGAGAT
TACCATTTTGTGACTTCAAGAGAACAGATGGAGAAAGATATCCAGGAGCA
TAAATTCATTGAAGCTGGCCAGTATAACAACCATCTATACGGAACGAGTG
TCCAATCTGTGCGAGCAGTAGCAGAGAAGGGCAAGCATTGTATCCTTGAT
GTGTCTGGAAATGCCATCAAGAGGTTGCAGATTGCACAGCTCTATCCAAT
ATCTACTTTTATTAAACCCAAATCCATGGAAAATATCATGGAAATGAATA
AACGCCTAACAGACGAACAGGCCAGAAAAACGTTTGAGAGAGCAGTGAGA
CTGGAGCAGGAGTTCACTGAGCATTTCACAGCTATTGTCCAGGGAGACAC
GCTGGAGGACATTTACAATCAAGTGAAGCAGATCATAGAAGAGCAGTCTG
GTCCTTACATCTGGGTCCCAGCAAAAGAAAAATTATGAAGATCT

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMPVRKQDTQRALHLLEEYRSKLNQ
TEDRQLRSSIERVISIFQSNLFQALIDIQEFYEVTLLDNPKCVDHSKQCE
PVQPGNPWESGSLSSAAVTSESLPGGLSPPVEKYRYQDEEVLPSERISPQ
VPNEVLGPELVHVSEKSLSEIENVHGFVSHSHISPIKANPPPVLVNTDSL
ETPTYVNGTDADYEYEEITLERGNSGLGFSIAGGTDNPHIGDDSSIFITK
IITGGAAAQDGRLRVNDCILRVNEADVRDVTHSKAVEALKEAGSIVRLYV
KRRKPASEKIMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGG
AAHKDGKLQIGDKLLAVNSVCLEEVTHEEAVTALKNTSDFVYLKVAKPTS
MYINDGYAPPDITNSSSQSVDNHVSPSSYLGQTPASPARYSPISKAVLGD
DEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELR
KGDRIISVNSVDLRAASHEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKI
HDLREQMMNSSVSSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNF
KFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKT
VKFNSKTRGDKGSFNDKRKKNLFSRKFPFYKNKDQSEQETSDADQHVTSN
ASDSESSYRGQEEYVLSYEPVNQQEVNYTRPVIILGPMKDRVNDDLISEF
PDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYN
NHLYGTSVQSVRAVAEKGKHCILDVSGNAIKRLQIAQLYPISTFIKPKSM
ENIMEMNKRLTDEQARKTFERAVRLEQEFTEHFTAIVQGDTLEDIYNQVK
QIIEEQSGPYIWVPAKEKL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU391
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expression. n.b. The current genbank reference differs greatly to the expressed protein