cDNA Clone Axin

• Plasmid: pGEX-Axin 489-862
• Parent Plasmid: pGEX6P1
• Expressed: GST-Axin 489-862
• Genbank: O15169
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCTGGCCATCGCTCCCCGGACAGTGGGCACGTGGCCAAGATGC
CAGTGGCACTGGGGGGTGCCGCCTCGGGGCACGGGAAGCACGTACCCAAG
TCAGGGGCGAAGCTGGACGCGGCCGGCCTGCACCACCACCGACACGTCCA
CCACCACGTCCACCACAGCACAGCCCGGCCCAAGGAGCAGGTGGAGGCCG
AGGCCACCCGCAGGGCCCAGAGCAGCTTCGCCTGGGGCCTGGAACCACAC
AGCCATGGGGCAAGGTCCCGAGGCTACTCAGAGAGTGTTGGCGCTGCCCC
CAACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTTGCGTGCA
AAAGAAATGCCAAGAAGGCTGAGTCGGGGAAGAGCGCCAGCACCGAGGTG
CCAGGTGCCTCGGAGGATGCGGAGAAGAACCAGAAAATCATGCAGTGGAT
CATTGAGGGGGAAAAGGAGATCAGCAGGCACCGCAGGACCGGCCACGGGT
CTTCGGGGACGAGGAAGCCACAGCCCCATGAGAACTCCAGACCCTTGTCC
CTTGAGCACCCCTGGGCCGGCCCTCAGCTCCGGACCTCCGTGCAGCCCTC
CCACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCTCCCAACC
CCCTAACCCAGCTGGAGGAGGCGCGCCGACGTCTGGAGGAGGAAGAAAAG
AGAGCCAGCCGAGCACCCTCCAAGCAGAGGTATGTGCAGGAGGTTATGCG
GCGGGGACGCGCCTGCGTCAGGCCAGCGTGCGCGCCGGTGCTGCACGTGG
TACCAGCCGTGTCGGACATGGAGCTCTCCGAGACAGAGACAAGATCGCAG
AGGAAGGTGGGCGGCGGGAGTGCCCAGCCGTGTGACAGCATCGTTGTGGC
GTACTACTTCTGCGGGGAACCCATCCCCTACCGCACCCTGGTGAGGGGCC
GCGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTGACCAAAAAGGGCAGC
TACAGATACTACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGGGTGGT
GTTTGAGGAGGTTCGAGAGGACGAGGCCGTCCTGCCCGTCTTTGAGGAGA
AGATCATCGGCAAAGTGGAGAAGGTGGACTGAGAATTC

Amino Acid Sequence

GPGHRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQSSFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVRPACAPVLHVVPAVSDMELSETETRSQRKVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU330
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion