cDNA Clone MEKK2

• Synonyms: MAP3K2
• Plasmid: pGEX-MEKK2
• Parent Plasmid: pGEX6P1
• Expressed: GST-MEKK2
• Genbank: AAD28547
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGATGATCAGCAAGCTTTGAACTCAATCATGCAAGATTTGGCTGTCCTTCATAAGGCCAGTCGACCAGCATTATCCTTGCAGGAAACCAGAAAAGCAAAATCTTCATCACCAAAAAAACAGAATGATGTCCGAGTCAAATTTGAACATAGAGGAGAAAAAAGAATCCTTCAGTTCCCCAGACCAGTTAAACTGGAAGATCTGAGATCTAAAGCTAAAATTGCCTTTGGACAATCTATGGATCTACATTATACCAATAACGAGTTGGTAATTCCATTAACTACTCAAGATGACTTGGACAAAGCTGTGGAACTGCTGGATCGTAGTATTCATATGAAGAGCCTCAAGATATTACTTGTAATAAATGGAAGTACACAGGCTACTAATTTAGAACCATTGCCATCACTAGAAGATTTGGATAATACAGTATTTGGAGCAGAGAGGAAAAAACGGCTATCTATAATAGGTCCTACTAGTAGAGATAGAAGTTCTCCTCCCCCAGGTTACATTCCAGATGAATTACACCAGGTTGCCCGGAATGGGTCATTCACTAGTATCAACAGTGAAGGAGAGTTCATTCCAGAGAGCATGGACCAAATGCTGGATCCATTATCTTTAAGCAGCCCTGAAAATTCTGGCTCAGGAAGTTGTCCATCACTTGATAGTCCTTTGGATGGAGAGAGCTATCCAAAATCACGAATGCCTAGGGCTCAGAGCTACCCAGATAATCATCAGGAATTTTCAGACTATGATAACCCTATCTTTGAGAAATTTGGAAAAGGAGGAACATATCCAAGAAGGTATCATGTTTCATATCATCATCAAGAGTATAATGATGGTCGTAAAACTTTTCCAAGAGCCAGAAGGACCCAGGGGACCAGCTTACGGTCTCCTGTGAGTTTCAGTCCTACTGATCATTCCTTAAGCACTAGTAGTGGAAGCAGTATCTTTACCCCAGAGTATGATGATAGTCGAATAAGAAGAAGGGGAAGTGACATAGACAATCCTACTTTGACCGTAATGGACATCAGCCCACCCAGCCGTTCACCTCGAGCTCCGACCAACTGGAGATTGGGCAAACTGCTTGGCCAAGGAGCCTTTGGAAGGGTCTACCTCTGTTATGATGTTGATACAGGAAGAGAATTGGCTGTTAAGCAAGTTCAATTTGACCCCGATAGTCCTGAGACCAGCAAGGAAGTAAATGCACTTGAGTGTGAAATTCAGTTGCTGAAAAACTTGCTACATGAGCGAATTGTTCAGTATTATGGCTGTTTGAGGGATCCCCAGGAAAAAACACTTTCCATATTTATGGAATATATGCCAGGGGGTTCAATTAAGGACCAATTAAAAGCATATGGCGCTCTTACTGAGAATGTGACTAGGAAATACACCCGTCAGATTCTGGAGGGTGTCCATTATTTGCACAGTAATATGATTGTCCATAGAGATATCAAAGGCGCAAATATCCTGCGAGATTCAACAGGCAACGTCAAACTAGGAGATTTTGGGGCCAGCAAACGGCTTCAGACCATCTGTCTCTCAGGGACAGGAATGAAGTCTGTCACGGGCACACCATACTGGATGAGCCCTGAAGTCATCAGTGGAGAAGGCTATGGAAGAAAAGCAGACATCTGGAGTGTTGCATGTACTGTGGTAGAAATGCTAACTGAAAAGCCGCCTTGGGCTGAATTTGAAGCAATGGCTGCCATCTTTAAAATCGCCACTCAGCCAACAAACCCAAAGCTGCCACCTCATGTCTCAGACTATACTCGAGATTTCCTCAAACGGATTTTTGTAGAGGCCAAACTGAGACCTTCAGCTGATGAACTCTTAAGGCACATGTTTGTGCATTATCACTAGGCGGCCGCAT

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFDDQQALNSIMQDLAVL
HKASRPALSLQETRKAKSSSPKKQNDVRVKFEHRGEKRILQFPRPVKLED
LRSKAKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKI
LLVINGSTQATNLEPLPSLEDLDNTVFGAERKKRLSIIGPTSRDRSSPPP
GYIPDELHQVARNGSFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSC
PSLDSPLDGESYPKSRMPRAQSYPDNHQEFSDYDNPIFEKFGKGGTYPRR
YHVSYHHQEYNDGRKTFPRARRTQGTSLRSPVSFSPTDHSLSTSSGSSIF
TPEYDDSRIRRRGSDIDNPTLTVMDISPPSRSPRAPTNWRLGKLLGQGAF
GRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLKNLLHER
IVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQI
LEGVHYLHSNMIVHRDIKGANILRDSTGNVKLGDFGASKRLQTICLSGTG
MKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAM
AAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSADELLRHMFVH
YH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU328
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST tag. MEKK2 was RT from HELA extract. Differs to the noted Genbank sequence L105V, E200D, G227D, K277Q, D278E, 279+Y, N294T, Q295S, T297R, F414L, G460V, L481V, Q537E