cDNA Clone MEKK1 (mouse)

• Synonyms: Map3k1
• Plasmid: pGEX-MEKK1
• Parent Plasmid: pGEX6P1
• Expressed: GST-MEKK1
• Genbank: L13103
• Tag: GST
• Species: Mouse

Sequence of Insert

ggatccgtgaccgcagtgcccgctgtgttttccaagctggtaaccatgcttaatgcttctggctccacccacttcaccaggatgcgccggcgtctgatggctatcgcggatgaggtagaaattgccgaggtcatccagctgggtgtggaggacactgtggatgggcatcaggacagcttacaggccgtggcccccaccagctgtctagaaaacagctcccttgagcacacagtccatagagagaaaactggaaaaggactaagtgctacgagactgagtgccagctcggaggacatttctgacagactggccggcgtctctgtaggacttcccagctcaacaacaacagaacaaccaaagccagcggttcaaacaaaaggcagaccccacagtcagtgtttgaactcctcccctttgtctcatgctcaattaatgttcccagcaccatcagccccttgttcctctgccccgtctgtcccagatatttctaagcacagaccccaggcatttgttccctgcaaaataccttccgcatctcctcagacacagcgcaagttctctctacaattccagaggaactgctctgaacaccgagactcagaccagctctccccagtcttcactcagtcaagacccccaccctccagtaacatacacaggccaaagccatcccgacccgttccgggcagtacaagcaaactaggggacgccacaaaaagtagcatgacacttgatctgggcagtgcttccaggtgtgacgacagctttggcggcggcggcaacagtggcaacgccgtcatacccagcgacgagacagtgttcacgccggtggaggacaagtgcaggttagatgtgaacaccgagctcaactccagcatcgaggaccttcttgaagcatccatgccttcaagtgacacgacagtcactttcaagtccgaagtcgccgtcctctctccggaaaaggccgaaaatgacgacacctacaaagacgacgtcaatcataatcaaaagtgcaaagaaaagatggaagctgaagaggaggaggctttagcgatcgccatggcgatgtcagcgtctcaggatgccctccccatcgtccctcagctgcaggtggaaaatggagaagatattatcatcattcagcaggacacaccagaaactcttccaggacataccaaagcgaaacagccttacagagaagacgctgagtggctgaaaggccagcagataggcctcggagcattttcttcctgttaccaagcacaggatgtggggactgggactttaatggctgtgaaacaggtgacgtacgtcagaaacacatcctccgagcaggaggaggtggtggaagcgttgagggaagagatccggatgatgggtcacctcaaccatccaaacatcatccggatgctgggggccacgtgcgagaagagcaactacaacctcttcattgagtggatggcgggaggatctgtggctcacctcttgagtaaatacggagctttcaaggagtcagtcgtcattaactacactgagcagttactgcgtggcctttcctatctccacgagaaccagatcattcacagagacgtcaaaggtgccaacctgctcattgacagcaccggtcagaggctgagaattgcagactttggagctgctgccaggttggcatcaaaaggaaccggtgcaggagagttccagggacagttactggggacaattgcattcatggcgcctgaggtcctaagaggtcagcagtatggtaggagctgtgatgtatggagtgttggctgcgccattatagaaatggcttgtgcaaaaccaccttggaatgcagaaaaacactccaatcatctcgccttgatatttaagattgctagcgcaactactgcaccgtccatcccgtcacacctgtccccgggtctgcgcgacgtggccgtgcgctgcttagaacttcagcctcaggaccggcctccgtccagagagctgctgaaacatccggtcttccgtaccacggtcgac

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSVTAVPAVFSKLVTMLNASG
STHFTRMRRRLMAIADEVEIAEVIQLGVEDTVDGHQDSLQAVAPTSCLEN
SSLEHTVHREKTGKGLSATRLSASSEDISDRLAGVSVGLPSSTTTEQPKP
AVQTKGRPHSQCLNSSPLSHAQLMFPAPSAPCSSAPSVPDISKHRPQAFV
PCKIPSASPQTQRKFSLQFQRNCSEHRDSDQLSPVFTQSRPPPSSNIHRP
KPSRPVPGSTSKLGDATKSSMTLDLGSASRCDDSFGGGGNSGNAVIPSDE
TVFTPVEDKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEK
AENDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQV
ENGEDIIIIQQDTPETLPGHTKAKQPYREDAEWLKGQQIGLGAFSSCYQA
QDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMGHLNHPNIIRML
GATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYL
HENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLL
GTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLAL
IFKIASATTAPSIPSHLSPGLRDVAVRCLELQPQDRPPSRELLKHPVFRT
TW*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU313
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion