cDNA Clone MTHFR

• Plasmid: pGFP MTHFR
• Parent Plasmid: pEGFPC1
• Expressed: pGFP MTHFR
• Genbank: U09806
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTGCCACCATGTACCCATACGATGTGCCAGATTTCGCCGTGAACGA
AGCCAGAGGAAACAGCAGCCTCAACCCCTGCTTGGAGGGCAGTGCCAGCA
GTGGCAGTGAGAGCTCCAAAGATAGTTCGAGATGTTCCATCCCGGGCCTG
GACCCCGAGCGGCATGAGAGACTCCGGGAGAAGATGAGGCGGCGATTGGA
ATCTGGTGACAAGTGGTTCTCCCTGGAATTCTTCCCTCCTCGAACTGTTG
AGGGAGCTGTCAATCTCATCTCAAGGTTTGACCGGATGGCAGCAGGTGGC
CCCCTCTACATAGACGTGAGCTGGCACCCAGCAGGTGACCCTGGCTCAGA
CAAGGAGACCTCCTCCATGATGATCGCCAGCACCACCGTGAACTACTGTG
GCCTGGAGACCATCCTGCACATGACCTGCTGCCGTCAGCGCCTGGAGGAG
ATCACGGGCCATCTGCACAAAGCTAAGCAGCTGGGCCTGAAGAACATCAT
GGCGCTGCGGGGAGACCCAATAGGTGACCAGTGGGAAGAGGAGGAGGGAG
GCTTCAACTACGCAGTGGACCTGGTGAAGCACATCCGAAGTGAGTTTGGT
GACTACTTTGACATCTGTGTGGCAGGTTACCCCAAAGGCCACCCCGAAGC
AGGGAGCTTTGAGGCTGACCTGAAGCACTTGAAGGAGAAGGTGTCTGCGG
GAGCCGATTTCATCATCACGCAGCTTTTCTTTGAGGCTGACACATTCTTC
CGCTTTGTGAAGGCATGCACCGACATGGGCATCACTTGCCCCATCGTCCC
CGGGATCTTTCCCATCCAGGGCTACCACTCCCTTCGGCAGCTTGTGAAGC
TGTCCAAGCTGGAGGTGCCACAGGAGATCAAGGACGTGATTGAGCCAATC
AAAGACAACGATGCTGCCATCCGCAACTATGGCATCGAGCTGGCCGTGAG
CCTGTGCCAGGAGCTTCTGGCCAGTGGCTTGGTGCCAGGCCTCCACTTCT
ACACCCTCAACCGCGAGATGGCTACCACAGAGGTGCTGAAGCGCCTGGGG
ATGTGGACTGAGGACCCCAGGCGTCCCCTACCCTGGGCTCTCAGCGCCCA
CCCCAAGCGCCGAGAGGAAGATGTACGTCCCATCTTCTGGGCCTCCAGAC
CAAAGAGTTACATCTACCGTACCCAGGAGTGGGACGAGTTCCCTAACGGC
CGCTGGGGCAATTCCTCTTCCCCTGCCTTTGGGGAGCTGAAGGACTACTA
CCTCTTCTACCTGAAGAGCAAGTCCCCCAAGGAGGAGCTGCTGAAGATGT
GGGGGGAGGAGCTGACCAGTGAAGCAAGTGTCTTTGAAGTCTTTGTTCTT
TACCTCTCGGGAGAACCAAACCGGAATGGTCACAAAGTGACTTGCCTGCC
CTGGAACGATGAGCCCCTGGCGGCTGAGACCAGCCTGCTGAAGGAGGAGC
TGCTGCGGGTGAACCGCCAGGGCATCCTCACCATCAACTCACAGCCCAAC
ATCAACGGGAAGCCGTCCTCCGACCCCATCGTGGGCTGGGGCCCCAGCGG
GGGCTATGTCTTCCAGAAGGCCTACTTAGAGTTTTTCACTTCCCGCGAGA
CAGCGGAAGCACTTCTGCGAGTGCTGAAGAAGTACGAGCTCCGGGTTAAT
TACCACCTTGTCAATGTGAAGGGTGAAAACATCACCAATGCCCCTGAACT
GCAGCCGAATGCTGTCACTTGGGGCATCTTCCCTGGGCGAGAGATCATCC
AGCCCACCGTAGTGGATCCCGTCAGCTTCATGTTCTGGAAGGACGAGGCC
TTTGCCCTGTGGATTGAGCGGTGGGGAAAGCTGTATGAGGAGGAGTCCCC
GTCCCGCACCATCATCCAGTACATCCACGACAACTACTTCCTGGTCAACC
TGGTGGACAATGACTTCCCACTGGACAACTGCCTCTGGCAGGTGGTGGAA
GACACATTGGAGCTTCTCAACAGGCCCACCCAGAATGCGAGAGAAACGGA
GGCTCCATGAAGATCT

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSATMYPYDVPDFAVNEARGNSSLNPCLEGSASSGSESSKD
SSRCSIPGLDPERHERLREKMRRRLESGDKWFSLEFFPPRTVEGAVNLIS
RFDRMAAGGPLYIDVSWHPAGDPGSDKETSSMMIASTTVNYCGLETILHM
TCCRQRLEEITGHLHKAKQLGLKNIMALRGDPIGDQWEEEEGGFNYAVDL
VKHIRSEFGDYFDICVAGYPKGHPEAGSFEADLKHLKEKVSAGADFIITQ
LFFEADTFFRFVKACTDMGITCPIVPGIFPIQGYHSLRQLVKLSKLEVPQ
EIKDVIEPIKDNDAAIRNYGIELAVSLCQELLASGLVPGLHFYTLNREMA
TTEVLKRLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRT
QEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSE
ASVFEVFVLYLSGEPNRNGHKVTCLPWNDEPLAAETSLLKEELLRVNRQG
ILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETAEALLRV
LKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPV
SFMFWKDEAFALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPL
DNCLWQVVEDTLELLNRPTQNARETEAP*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU298
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian construct to express GFP/HA N terminal fusion.