cDNA Clone ERK5

• Synonyms: MAPK7
• Plasmid: pGEX6P-1-ERK5
• Parent Plasmid: pGEX6P1
• Expressed: GST-ERK5
• Genbank: Q13164
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCGAGCCTCTGAAGGAGGAAGACGGCGAGGACGGCTCTGCGGA
GCCCCCCGGGCCCGTGAAGGCCGAACCCGCCCACACCGCTGCCTCTGTAG
CGGCCAAGAACCTGGCCCTGCTTAAAGCCCGCTCCTTCGATGTGACCTTT
GACGTGGGCGACGAGTACGAGATCATCGAGACCATAGGCAACGGGGCCTA
TGGAGTGGTGTCCTCCGCCCGCCGCCGCCTCACCGGCCAGCAGGTGGCCA
TCAAGAAGATCCCTAATGCTTTCGATGTGGTGACCAATGCCAAGCGGACC
CTCAGGGAGCTGAAGATCCTCAAGCACTTTAAACACGACAACATCATCGC
CATCAAGGACATCCTGAGGCCCACCGTGCCCTATGGCGAATTCAAATCTG
TCTACGTGGTCCTGGACCTGATGGAAAGCGACCTGCACCAGATCATCCAC
TCCTCACAGCCCCTCACACTGGAACACGTGCGCTACTTCCTGTACCAACT
GCTGCGGGGCCTGAAGTACATGCACTCGGCTCAGGTCATCCACCGTGACC
TGAAGCCCTCCAACCTATTGGTGAATGAGAACTGTGAGCTCAAGATTGGT
GACTTTGGTATGGCTCGTGGCCTGTGCACCTCGCCCGCTGAACATCAGTA
CTTCATGACTGAGTATGTGGCCACGCGCTGGTACCGTGCGCCCGAGCTCA
TGCTCTCTTTGCATGAGTATACACAGGCTATTGACCTCTGGTCTGTGGGC
TGCATCTTTGGTGAGATGCTGGCCCGGCGCCAGCTCTTCCCAGGCAAAAA
CTATGTACACCAGCTACAGCTCATCATGATGGTGCTGGGTACCCCATCAC
CAGCCGTGATTCAGGCTGTGGGGGCTGAGAGGGTGCGGGCCTATATCCAG
AGCTTGCCACCACGCCAGCCTGTGCCCTGGGAGACAGTGTACCCAGGTGC
CGACCGCCAGGCCCTATCACTGCTGGGTCGCATGCTGCGTTTTGAGCCCA
GCGCTCGCATCTCAGCAGCTGCTGCCCTTCGCCACCCTTTCCTGGCCAAG
TACCATGATCCTGATGATGAGCCTGACTGTGCCCCGCCCTTTGACTTTGC
CTTTGACCGCGAAGCCCTCACTCGGGAGCGCATTAAGGAGGCCATTGTGG
CTGAAATTGAGGACTTCCATGCAAGGCGTGAGGGCATCCGCCAACAGATC
CGCTTCCAGCCTTCTCTACAGCCTGTGGCTAGTGAGCCTGGCTGTCCAGA
TGTTGAAATGCCCAGTCCCTGGGCTCCCAGTGGGGACTGTGCCATGGAGT
CTCCACCACCAGCCCCGCCACCATGCCCCGGCCCTGCACCTGACACCATT
GATCTGACCCTGCAGCCACCTCCACCAGTCAGTGAGCCTGCCCCACCAAA
GAAAGATGGTGCCATCTCAGACAATACTAAGGCTGCCCTTAAAGCTGCCC
TGCTCAAGTCTTTGAGGAGCCGGCTCAGAGATGGCCCCAGCGCACCCCTG
GAGGCTCCTGAGCCTCGGAAGCCGGTGACAGCCCAGGAGCGCCAGCGGGA
GCGGGAGGAGAAGCGGCGGAGGCGGCAAGAACGAGCCAAGGAGCGGGAGA
AACGGCGGCAGGAGCGGGAGCGAAAGGAACGGGGGGCTGGGGCCTCTGGG
GGCCCCTCCACTGACCCCTTGGCTGGACTAGTGCTCAGTGACAATGACAG
AAGCCTGTTGGAACGCTGGACTCGAATGGCCCGGCCCGCAGCCCCAGCCC
TCACCTCTGTGCCGGCCCCTGCCCCAGCGCCAACGCCAACCCCAACCCCA
GTCCAACCTACCAGTCCTCCTCCTGGCCCTGTAGCCCAGCCCACTGGCCC
GCAACCACAATCTGCGGGCTCTACCTCTGGCCCTGTACCCCAGCCTGCCT
GCCCACCCCCTGGCCCTGCACCCCACCCCACTGGCCCTCCTGGGCCCATC
CCTGTCCCCGCGCCACCCCAGATTGCCACCTCCACCAGCCTCCTGGCTGC
CCAGTCACTTGTGCCACCCCCTGGGCTGCCTGGCTCCAGCACCCCAGGAG
TTTTGCCTTACTTCCCACCTGGCCTGCCGCCCCCAGACGCCGGGGGAGCC
CCTCAGTCTTCCATGTCAGAGTCACCTGATGTCAACCTTGTGACCCAGCA
GCTATCTAAGTCACAGGTGGAGGACCCCCTGCCCCCTGTGTTCTCAGGCA
CACCAAAGGGCAGTGGGGCTGGCTACGGTGTTGGCTTTGACCTGGAGGAA
TTCTTAAACCAGTCTTTCGACATGGGCGTGGCTGATGGGCCACAGGATGG
CCAGGCAGATTCAGCCTCTCTCTCAGCCTCCCTGCTTGCTGACTGGCTCG
AAGGCCATGGCATGAACCCTGCCGATATTGAGTCCCTGCAGCGTGAGATC
CAGATGGACTCCCCAATGCTGCTGGCTGACCTGCCTGACCTCCAGGACCC
CTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAEPLKEEDGEDGSAEPPAR
EGRTRPHRCLCSAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVSS
ARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDIL
RPTVPYGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLK
YMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTSPAEHQYFMTEY
VATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQL
QLIMMVLGTPSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQAL
SLLGRMLRFEPSARISAAAALRHPFLAKYHDPDDEPDCAPPFDFAFDREA
LTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPGCPDVEMPS
PWAPSGDCAMESPPPAPPPCPGPAPDTIDLTLQPPPPVSEPAPPKKDGAI
SDNTKAALKAALLKSLRSRLRDGPSAPLEAPEPRKPVTAQERQREREEKR
RRRQERAKEREKRRQERERKERGAGASGGPSTDPLAGLVLSDNDRSLLER
WTRMARPAAPALTSVPAPAPAPTPTPTPVQPTSPPPGPLAQPTGPQPQSA
GSTSGPVPQPACPPPGPAPHPTGPPGPIPVPAPPQIATSTSLLAAQSLVP
PPGLPGSSTPGVLPYFPPGLPPPDAGGAPQSSMSESPDVNLVTQQLSKSQ
VEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSA
SLSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU288
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion