cDNA Clone Cardiac PFK2

• Plasmid: pCMV-HA PFK2
• Parent Plasmid: pCMV5
• Expressed: HA-PFK2
• Genbank: CAA06605
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCTCTGGGGC
ATCTTCCTCAGAACAGAACAACAACAGCTATGAAACCAAAACCCCAAATC
TTCGAACAGAGAAGAAATCATGGGCCTCCTACATGACCAACTCCCCGACT
CTGATCGTTATGATTGGTTTGCCAGCCCGGGGTAAAACCTACGTGTCCAA
GAAACTAACACGCTACCTCAACTGGATTGGAGTCCCCACCAAAGTGTTTA
ATCTTGGGGTGTATCGGCGTGAAGCAGTCAAGTCCTATAAGTCCTACGAC
TTCTTTCGGCATGACAATGAGGAGGCCATGAAGATCCGCAAACAGTGTGC
TCTGGTGGCGCTGGAAGATGTTAAGGCGTATCTCACTGAGGAGAATGGTC
AGATTGCGGTGTTTGATGCCACCAATACAACCCGGGAGAGGAGGGACATG
ATTTTGAACTTTGCTGAACAGAATTCCTTCAAGGTATTCTTTGTGGAATC
CGTCTGTGATGATCCTGATGTCATTGCTGCCAATATTCTGGAGGTTAAGG
TATCAAGCCCTGACTATCCTGAAAGGAACAGAGAGAACGTGATGGAGGAC
TTCCTGAAGAGAATTGAATGCTACAAAGTTACCTACCGACCTCTTGACCC
AGACAACTATGACAAGGATCTTTCTTTCATCAAGGTGATAAACGTGGGCC
AGCGATTTTTAGTCAACAGAGTCCAGGACTACATCCAGAGCAAGATAGTC
TACTACCTCATGAATATCCACGTCCAGCCTCGCACCATTTACCTTTGCCG
GCATGGAGAAAGCGAGTTCAATCTCTTGGGGAAGATTGGGGGTGACTCTG
GCCTCTCGGTGCGGGGAAAGCAGTTTGCCCAAGCTCTAAGGAAATTTCTG
GAGGAACAGGAAATAACAGACCTCAAAGTGTGGACAAGCCAGTTGAAGAG
GACCATACAGACTGCTGAATCTCTCGGGGTGCCCTATGAGCAGTGGAAGA
TTCTGAATGAGATTGATGCTGGTGTGTGTGAAGAGATGACCTATGCAGAG
ATTGAGAAACGGTACCCAGAAGAGTTTGCACTTCGAGATCAAGAGAAGTA
TCTGTATCGATATCCTGGTGGGGAGTCATACCAGGACCTGGTGCAGCGGC
TGGAGCCTGTCATCATGGAGCTGGAACGTCAGGGCAATGTCCTCGTCATC
TCCCACCAGGCTGTCATGCGCTGCCTCCTGGCCTACTTCTTGGATAAGGG
CGCAGATGAGCTACCATACTTGAGATGCCCTCTCCATACCATCTTCAAAC
TTACTCCTGTGGCCTATGGGTGCAAAGTGGAAACAATTAAACTTAACGTG
GAGGCTGTGAACACGCACCGTGACAAGCCAACTAACAACTTCCCCAAGAA
CCAAACCCCTGTAAGGATGAGAAGGAACAGCTTTACGCCTCTGTCCAGTT
CGAATACAATAAGGCGTCCAAGAAATTACAGTGTTGGGAGCCGGCCCCTC
AAGCCCCTCAGCCCTCTCCGTGCCCAGGACATGCAAGAAGGGGCCGACTA
GGGATCC

Amino Acid Sequence

MYPYDVPDYASGASSSEQNNNSYETKTPNLRTEKKSWASYMTNSPTLIVM
IGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYKSYDFFRH
DNEEAMKIRKQCALVALEDVKAYLTEENGQIAVFDATNTTRERRDMILNF
AEQNSFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFLKR
IECYKVTYRPLDPDNYDKDLSFIKVINVGQRFLVNRVQDYIQSKIVYYLM
NIHVQPRTIYLCRHGESEFNLLGKIGGDSGLSVRGKQFAQALRKFLEEQE
ITDLKVWTSQLKRTIQTAESLGVPYEQWKILNEIDAGVCEEMTYAEIEKR
YPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNVLVISHQA
VMRCLLAYFLDKGADELPYLRCPLHTIFKLTPVAYGCKVETIKLNVEAVN
THRDKPTNNFPKNQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSRPLKPLS
PLRAQDMQEGAD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU284
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian construct to express HA N terminal fusion. N.B. has ΔM23