cDNA Clone Cyclin D1

• Plasmid: pGEX-Cyclin D1
• Parent Plasmid: pGEX6P1
• Expressed: GST-Cyclin D1
• Genbank: NM_053056
• Tag: GST
• Species: Human

Sequence of Insert

ggatccGAACACCAGCTCCTGTGCTGCGAAGTGGAAACCATCCGCCGCGCGTACCCCGATGCCAACCTCCTCAACGACCGGGTGCTGCGGGCCATGCTGAAGGCGGAGGAGACCTGCGCGCCCTCGGTGTCCTACTTCAAATGTGTGCAGAAG GAGGTCCTGCCGTCCATGCGGAAGATCGTCGCCACCTGGATGCTGGAGGT CTGCGAGGAACAGAAGTGCGAGGAGGAGGTCTTCCCGCTGGCCATGAACT ACCTGGACCGCTTCCTGTCGCTGGAGCCCGTGAAAAAGAGCCGCCTGCAG CTGCTGGGGGCCACTTGCATGTTCGTGGCCTCTAAGATGAAGGAGACCAT CCCCCTGACGGCCGAGAAGCTGTGCATCTACACCGACAACTCCATCCGGC CCGAGGAGCTGCTGCAAATGGAGCTGCTCCTGGTGAACAAGCTCAAGTGG AACCTGGCCGCAATGACCCCGCACGATTTCATTGAACACTTCCTCTCCAA AATGCCAGAGGCGGAGGAGAACAAACAGATCATCCGCAAACACGCGCAGA CCTTCGTTGCCCTCTGTGCCACAGATGTGAAGTTCATTTCCAATCCGCCC TCCATGGTGGCAGCGGGGAGCGTGGTGGCCGCAGTGCAAGGCCTGAACCT GAGGAGCCCCAACAACTTCCTGTTCTACTACCGCCTCACACGCTTCCTCT CCAGAGTGATCAAGTGTGACCCAGACTGCCTCCGGGCCTGCCAGGAGCAG ATCGAAGCCCTGCTGGAGTCAAGCCTGCGCCAGGCCCAGCAGAACATGGA CCCCAAGGCCGCCGAGGAGGAGGAAGAGGAGGAGGAGGAGGTGGACCTGG CTTGCACACCCACCGACGTGCGGGACGTGGACATCTGAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEHQLLCCEVETIRRAYPDA NLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEE QKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLT AEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPE AEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSP NNFLFYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKA AEEEEEEEEEVDLACTPTDVRDVDI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU274
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence difference compared with NM_053056 CDS: c674t (S225F). Estimated MW of expressed protein : 60442 Daltons