cDNA Clone PPP4R2

• Plasmid: pGFP PPP4R2-1
• Parent Plasmid: pEGFPC1
• Expressed: pGFP PPP4R2-1
• Genbank: NM_019853
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACTACAAGGACGACGATGACAAGTGCCAGGCGCC
ATGTTGGAGGGCTGGTGGTAGCGGCTTGGGGAGGTGCTCACTCTGTCGGT
CTTGCTCTCTCGCACGCTTCCCCCGGCTCCCTTCGTTTCCCCCCCCCGGT
CGCCTGCGTGCCGGAGTGTGTGCGAGGGAGGGGGAGGGCGTCGGGGGGGT
GGGGGGAGGCGTTCCGGTCCCCAAAAGACCCGCGGAGGGAGGCGGAGGCT
GTGAGGGACTCCGGGAAGCCATGGACGTCGAGAGGCTCCAGGAGGCGCTG
AAAGATTTTGAGAAGAGGGGGAAAAAGGAAGTTTGTCCTGTCCTGGATCA
GTTTCTTTGTCATGTAGCCAAGACTGGAGAAACAATGATTCAGTGGTCCC
AATTTAAAGGCTATTTTATTTTCAAACTGGAGAAAGTGATGGATGATTTC
AGAACTTCAGCTCCTGAGCCAAGAGGTCCTCCCAACCCTAATGTCGAATA
TATTCCCTTTGATGAAATGAAGGAAAGAATACTGAAAATTGTCACTGGAT
TTAATGGTATCCCTTTTACTATTCAGCGACTATGTGAATTGTTAACAGAT
CCAAGGAGAAACTATACAGGAACAGACAAATTTCTCAGAGGAGTAGAAAA
GAACGTGATGGTTGTTAGCTGTGTTTATCCTTCTTCAGAGAGAAACAATT
CCAATAGTTTAAATCGAATGAATGGTGTGATGTTTCCTGGAAATGCACCA
AGCTATACTGAGAGGTCTAATATAAATGGGCCTGGGACACCCAGGCCACG
TAATCGACCAAAGGTTTCTCTGTCAGCCCCCATGACAACAAATGGGTGGC
CTGAGAGCACAGACAGCAAAGAGGCAAATTTGCAGCAAAATGAGGAGAAA
ACTCACAGTGACTCTTCGACATCTGAATCAGAAGTTTCCTCAGTGAGCCC
TTTGAGAAATAAACATCCAGATGAAGATGCTGTGGAAGCTGAGGGGCATG
AGGTAAAAAGACTCAGGTTTGACAAAGAAGGTGAAGTCAGAGAAACAGCC
AGTCAAACGACTTCCAGCGAAATTTCTTCAGTTATGGTAGGAGAAACAGA
AGCATCATCTTCATCTCAGGATAAAGACAAAGATAGCCGTTGTACCCGGC
AGCACTGTACAGAAGAGGATGAAGAAGAGGATGAAGAGGAAGAAGAAGAG
TCTTTTATGACATCAAGAGAAATGATCCCAGAAAGAAAAAATCAAGAAAA
AGAATCTGATGATGCCTTAACTGTGAATGAAGAGACTTCTGAAGAAAATA
ATCAAATGGAGGAATCTGATGTGTCTCAAGCTGAGAAAGATTTGCTACAT
TCTGAAGGTAGTGAAAACGAAGGCCCTGAAAGTAAGTGGTTCTTCTGAGT
CGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSATMDYK
DDDDKCQAPCWRAGGSGLGRCSLCRSCSLARFPRLPSFPPPGRLRAGVCA
REGEGVGGVGGGVPVPKRPAEGGGGCEGLREAMDVERLQEALKDFEKRGK
KEVCPVLDQFLCHVAKTGETMIQWSQFKGYFIFKLEKVMDDFRTSAPEPR
GPPNPNVEYIPFDEMKERILKIVTGFNGIPFTIQRLCELLTDPRRNYTGT
DKFLRGVEKNVMVVSCVYPSSERNNSNSLNRMNGVMFPGNAPSYTERSNI
NGPGTPRPRNRPKVSLSAPMTTNGWPESTDSKEANLQQNEEKTHSDSSTS
ESEVSSVSPLRNKHPDEDAVEAEGHEVKRLRFDKEGEVRETASQTTSSEI
SSVMVGETEASSSSQDKDKDSRCTRQHCTEEDEEEDEEEEEESFMTSREM
IPERKNQEKESDDALTVNEETSEENNQMEESDVSQAEKDLLHSEGSENEG
PESKWFF*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU258
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/FLAG for mammalian expression; Calc Mol weight 79008 Da